Metallomics and metalloproteomics

Photosubstitution and photoreduction of a diazido platinum(iv) anticancer complex

“…[24][25][26][27] Reverse phase high performance liquid chromatography (RP-HPLC) is a most frequently used separation technique, due to its promising capability for separating a wide range of compounds. 28,29 The combination of HPLC and ICP-MS provides the potential for the accurate analysis of the products from interaction of metallodrugs with small biomolecules, 30 their binding to proteins by (metallo)proteomics, 31,32 as well as the dark stability and metabolism of metallodrugs in human plasma and cells at nanomolar concentrations. 26,[33][34][35] Investigations of classical platinum drugs using HPLC-ICP-MS have been widely reported, including their stability in biofluids, interactions with biomolecules, and adducts within cells, but photoactive diazido Pt(IV) complexes have not yet been fully explored.…”

“…24–27 Reverse phase high performance liquid chromatography (RP-HPLC) is a most frequently used separation technique, due to its promising capability for separating a wide range of compounds. 28,29 The combination of HPLC and ICP-MS provides the potential for the accurate analysis of the products from interaction of metallodrugs with small biomolecules, 30 their binding to proteins by (metallo)-proteomics, 31,32 as well as the dark stability and metabolism of metallodrugs in human plasma and cells at nanomolar concentrations. 26,33–35…”