2020
DOI: 10.1002/rcm.8734
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Metastable decomposition at the peptide C‐terminus: Possible use in protein identification

Abstract: Rationale:The b n−1 ion of a peptide, as well as a [b n−1 + 18] ion, can be observed not only as normal product ions, but also as prominent metastable ions in a reflectronembedded matrix-assisted laser desorption ionization time-of-flight spectrometer.The m/z values for the peaks are slightly shifted compared with the ordinary product ions and appear as relatively broad peaks, which permits them to be discriminated from other ions. Methods:A standard protein mixture and gel-derived proteins digested with LysN … Show more

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Cited by 3 publications
(2 citation statements)
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“…Brown and Wilkins [50] indicate that metastable chlorophyll ions arise from excess energy supplied during desorption. Several parameters can influence MALDI's metastable ion formation, such as energy absorption directly from the laser, collision with matrix molecules, ion acceleration through the source's electrical field, and exothermic reactions in the MALDI plume [48 , 51 , 52] . The metastable ion at m/z 672.9 ( Fig.…”
Section: Methods Validationmentioning
confidence: 99%
“…Brown and Wilkins [50] indicate that metastable chlorophyll ions arise from excess energy supplied during desorption. Several parameters can influence MALDI's metastable ion formation, such as energy absorption directly from the laser, collision with matrix molecules, ion acceleration through the source's electrical field, and exothermic reactions in the MALDI plume [48 , 51 , 52] . The metastable ion at m/z 672.9 ( Fig.…”
Section: Methods Validationmentioning
confidence: 99%
“…Each fraction was spotted onto a stainless steel plate, dried, and mixed with a matrix solution (0.5 µL per spot; 7 mg/mL of α-cyano-4-hydroxycinnamic acid (Sigma) in 50% ACN, 0.1% TFA). MALDI-TOFMS and MS/MS measurements were carried out with a 4700 MALDI-TOF/TOF mass spectrometer (Applied Biosystems) as previously described 57 . All mass spectra were obtained by averaging 2500 laser shots from each sample well in positive-ion mode.…”
Section: Methodsmentioning
confidence: 99%