2016
DOI: 10.1016/j.vph.2016.01.002
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Metformin alleviates vascular calcification induced by vitamin D3 plus nicotine in rats via the AMPK pathway

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Cited by 28 publications
(30 citation statements)
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“…Immunohistochemical staining was performed as previously described . Briefly, tissue sections (5 μm) were rehydrated, and heat‐mediated antigen retrieval was performed using 0.05 mol/L sodium citrate buffer (pH 6.0).…”
Section: Methodsmentioning
confidence: 99%
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“…Immunohistochemical staining was performed as previously described . Briefly, tissue sections (5 μm) were rehydrated, and heat‐mediated antigen retrieval was performed using 0.05 mol/L sodium citrate buffer (pH 6.0).…”
Section: Methodsmentioning
confidence: 99%
“…Western blot analysis was performed as previously described . Primary antibodies against Sp1 (0.1 μg/mL, Millipore), BMP2 (1:1000 dilution), calponin (1:2000 dilution) (Proteintech Group, Chicago, IL), Runx2 (1:1000 dilution), α‐SMA (1:1000 dilution), caspase‐3 (1:1000 dilution), β‐actin (1:1000 dilution), GAPDH (1:1000 dilution) (Cell Signaling Technology), and horseradish peroxidase‐conjugated secondary antibody (Beijing Zhong Shan Golden Bridge Biological Technology, China) were used.…”
Section: Methodsmentioning
confidence: 99%
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“…However, some researchers found that metformin of high dose (2 mM) can obviously suppress osteoblast differentiation when AMPK was invoked . Moreover, metformin has been proved to prevent vascular calcification via AMPK pathway . However, there are no reports on the application of metformin for antiosteogenesis.…”
Section: Introductionmentioning
confidence: 99%