Metformin and Glibenclamide

Analysis of the second generation antidepressant venlafaxine and its main active metabolite O-desmethylvenlafaxine in human plasma by HPLC with spectrofluorimetric detection

“…In fact, the SPE procedures are more feasible, faster and require lower volumes of organic solvents. Extraction yield values also are higher than those reported by other authors [20] and precision values are better than those reported in most other papers [17,18,[20][21][22][23][24]. The proposed method is also advantageous because it has high accuracy and a wide linearity range.…”

“…The fluorimetric detection and the SPE procedure together make the method selective: neither endogenous compound nor any of the central nervous system drugs tested has caused any interference in the therapeutic monitoring of VLX and ODV in depressed patient plasma. The use of SPE poses several advantages with respect to the liquid-liquid extraction procedures used by other authors [17][18][19][20][21][22][23][24][25]. In fact, the SPE procedures are more feasible, faster and require lower volumes of organic solvents.…”

“…Several methods can be found in the literature for the determination of therapeutic levels of VLX and ODV in human plasma or serum [17][18][19][20][21][22][23][24][25]. Most of them use HPLC coupled to spectrofluorimetric [21,23], spectrophotometric [18,22,24], electrochemical (coulometric) [20] or mass spectrometric [17] detection.…”

“…Most of them use HPLC coupled to spectrofluorimetric [21,23], spectrophotometric [18,22,24], electrochemical (coulometric) [20] or mass spectrometric [17] detection. Capillary electrophoresis with UV detection has been used as well [19,24].…”

“…This has the disadvantage of using relatively high volumes of polluting and dangerous organic solvents and of being quite labour intensive and timeconsuming. Furthermore, several methods [18,19,[21][22][23][24] have limits of quantification (LOQ) values in the 5-50 ng mL −1 range. While they can determine therapeutic levels of VLX and ODV, they are not suitable for the TDM of patients receiving low doses or in cases of low compliance.…”