Methacrylamide-modified collagen hydrogel with improved anti-actin-mediated matrix contraction behavior

Yang, Ke; Sun, Jing; Guo, Zhenzhen; Yang, Jun; Wei, Dan; Tan, Yanfei; Guo, Likun; Luo, Hongrong; Fan, Hongsong; Zhang, Xingdong

doi:10.1039/c8tb02314j

Cited by 37 publications

(33 citation statements)

References 58 publications

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“…Different research approaches may exploit different crosslinking mechanisms for collagenbased bioinks. The use of photochemical modifications can be applied to provide increased mechanical performance and stability of the construct and promote chondrogenic differentiation [37]. Another possibility is the use of chemical crosslinking agents [27] which are known to often cause cytotoxicity effects [27] but have been demonstrated to be compatible with cell activity in some cases (use of tannic acid in combination with a cell-laden collagen hydrogel) [38].…”

Section: Discussionmentioning

confidence: 99%

Cartilage Tissue Engineering by Extrusion Bioprinting: Process Analysis, Risk Evaluation, and Mitigation Strategies

et al. 2021

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Extrusion bioprinting is considered promising in cartilage tissue engineering since it allows the fabrication of complex, customized, and living constructs potentially suitable for clinical applications. However, clinical translation is often complicated by the variability and unknown/unsolved issues related to this technology. The aim of this study was to perform a risk analysis on a research process, consisting in the bioprinting of a stem cell-laden collagen bioink to fabricate constructs with cartilage-like properties. The method utilized was the Failure Mode and Effect Analysis/Failure Mode and Effect Criticality Analysis (FMEA/FMECA) which foresees a mapping of the process to proactively identify related risks and the mitigation actions. This proactive risk analysis allowed the identification of forty-seven possible failure modes, deriving from seventy-one potential causes. Twenty-four failure modes displayed a high-risk level according to the selected evaluation criteria and threshold (RPN > 100). The results highlighted that the main process risks are a relatively low fidelity of the fabricated structures, unsuitable parameters/material properties, the death of encapsulated cells due to the shear stress generated along the nozzle by mechanical extrusion, and possible biological contamination phenomena. The main mitigation actions involved personnel training and the implementation of dedicated procedures, system calibration, printing conditions check, and, most importantly, a thorough knowledge of selected biomaterial and cell properties that could be built either through the provided data/scientific literature or their preliminary assessment through dedicated experimental optimization phase. To conclude, highlighting issues in the early research phase and putting in place all the required actions to mitigate risks will make easier to develop a standardized process to be quickly translated to clinical use.

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Section: Discussionmentioning

confidence: 99%

Cartilage Tissue Engineering by Extrusion Bioprinting: Process Analysis, Risk Evaluation, and Mitigation Strategies

et al. 2021

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“…[54] A recent study shows that the bioscaffolds printed with CMA-based hydrogel promote the proliferation and chondrogenic differentiation of mesenchymal stem cells (MSCs) in cartilage regeneration. [55] Gelatin is derived from the hydrolysis of collagen at high temperature. [56,57] Gelatin contains many arginineÀglycineÀaspartic acid (RGD) sequences that favor the adhesion, proliferation, and differentiation of cells.…”

Section: Protein-based Biomaterialsmentioning

confidence: 99%

Advances in Photocrosslinkable Materials for 3D Bioprinting

Zhang

Yan

2021

Adv Eng Mater

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3D bioprinting integrating multidisciplinary advances in engineering, cell biology, and material science is able to fabricate artificial tissues and organ‐like structures with biology or clinic‐relevant size, shape, and structural integrity. Therefore, 3D bioprinting has huge potential in tissue engineering and regenerative medicine. Suitable bioink plays a key role in the modulation of the printing process, the properties, and functions of printed constructs. Herein, the advances in photocrosslinkable biomaterials are focused on, which have been proven as promising types of bioinks for 3D bioprinting. The general strategies of 3D bioprinting and the main mechanisms involved in photocrosslinking are first discussed. Then, the recent advances in photocrosslinkable bioinks including natural protein‐ and polysaccharide‐based materials, synthetic polymers, and decellularized extracellular matrix (dECM)‐based materials are highlighted. Finally, the ongoing challenges in the delicate balance between the printability and biological activities of photocrosslinkable biomaterials are discussed and the outlook for this emerging area is analyzed.

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“…Additionally, a study investigating different types of crosslinking for HA gels, showed that the stiffest formulation at 1.8 kPa, using 4-armed PEG crosslinking, was most optimal for BMSC chondrogenesis (37). Another study exploring hydrogel crosslinking options, showed that 9 kPa photo-crosslinked collagen I gels coincided with enhanced rabbit BMSC chondrogenesis both in vitro and in vivo in a mouse model, compared to softer, physically crosslinked 2 kPa gels (138). This study also highlighted the importance of actin-mediated matrix interactions during chondrogenesis by showing that inhibition of actin polymerisation with cytochalasin D worsened differentiation (138).…”

Section: Stiffnessmentioning

confidence: 99%

ChondroGELesis: Hydrogels to harness the chondrogenic potential of stem cells

Walker

Luo

Pringle

et al. 2021

Materials Science and Engineering: C

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Methacrylamide-modified collagen hydrogel with improved anti-actin-mediated matrix contraction behavior

Abstract: A collagen-based chondro-inductive hydrogel with improved mechanical properties was developed to promote chondrogenesis of BMSCs by resisting actin-mediated matrix contraction.

Cited by 37 publications

References 58 publications

Cartilage Tissue Engineering by Extrusion Bioprinting: Process Analysis, Risk Evaluation, and Mitigation Strategies

Cartilage Tissue Engineering by Extrusion Bioprinting: Process Analysis, Risk Evaluation, and Mitigation Strategies

Advances in Photocrosslinkable Materials for 3D Bioprinting

ChondroGELesis: Hydrogels to harness the chondrogenic potential of stem cells

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