Methacrylate‐based short monolithic columns: Enabling tools for rapid and efficient analyses of biomolecules and nanoparticles

Abstract

This review describes the novel chromatography stationary phase--a porous monolithic methacrylate-based polymer--in terms of the design of the columns and some of the features that make these columns attractive for the purification of large biomolecules. We first start with a brief summary of the characteristics of these large molecules (more precisely large proteins like immunoglobulins G and M, plasmid deoxyribonucleic acid (DNA), and viral particles), and a list of some of the problems that were encountered during the development of efficient purification processes. We then briefly describe the structure of the methacrylate-based monolith and emphasize the features which make them more than suitable for dealing with large entities. The highly efficient structure on a small scale can be transferred to a large scale without the need of making column modifications, and the various approaches of how this is accomplished are briefly presented in this paper. This is followed by presenting some of the examples from the bioprocess development schemes, where the implementation of the methacrylate-based monolithic columns has resulted in a very efficient and productive process. Following this, we move back to the analytical scale and demonstrate the efficiency of the monolithic column--where the mass transfer between the stationary and mobile phase is greatly enhanced--for the in-process and final control of the new therapeutics. The combination of an efficient structure and the appropriate hardware results in separations of proteins with residence time less than 0.1 s.

Possible Association of Altered Serum Lipids in Obese Adult Females with Adenoviral Infection

“…But further research with use of measurements of the serotype-specific neutralizing antibodies to adenovirus or isolation/identification of specific adenovirus like particles in serum and adipose tissue samples using convective interaction media (CIM) monolithic column technology [50] in this group of OW/OB females should be performed.…”

Monoliths in Bioprocess Technology

“…Another main advantage of monoliths is their flow-independent DBC allowing straight-forward scalability [3,[26][27][28]. A direct comparison between monoliths and particle-based resins for the purification of virus-like particles from yeast homogenate has been conducted by Burden et al The authors concluded that the monoliths are three-fold superior in terms of DBC with equivalent recovery in yield (90%) compared to particle-based resins [29].…”

“…Monolithic supports are easy to scale up without the need for column modifications [3,26]. Monolithic disks can be stacked up to four disks in the same housing, increasing efficiency and capacity without compromising resolution [3].…”

The Value of Fungal Protease Inhibitors in Affinity Chromatography

“…Monolith is a name for the chromatographic stationary phase consisting of a single piece of highly porous material (Barut et al, 2008). They are prepared in various dimensions with agglomeration-type or fibrous microstructures.…”