Methane yield and microscopic observation as monitoring biofilm behaviour parameters, during start up phase of anaerobic inverse fluidized bed reactor

Abstract

Anaerobic biofilm behavior on polyethylene and Extendosphere™ supports was evaluated during startup of an inverse fluidized bed reactor using methane yield and microscopic observation as parameter monitoring techniques. Two anaerobic inverse fluidized bed reactors were used, one filled with triturated polyethylene as solid carrier material (diameter = 380 m, density = 926 kg/m 3) and the other with Extendosphere™ (diameter = 147 m, density = 700 kg/m 3). Each support material was used at up to 25% of its working volume (polyethylene = 1.2 l, Extendosphere™ = 1.9 l). Both reactors were started up in sequencing batch mode, applying organic loading rates of 0.5 to 14 g COD/l.d. Both supports exhibited rapid biofilm growth during start-up. Maximum surface colonization was 46% with the polyethylene and 100% with Extendosphere™. Both supports had a methane yield of 0.298 l CH 4 /g COD at 10 and 14 g COD/l.d, respectively. Digital microscopic observation results coincided with methane yield results, confirming each to be viable for parameter monitoring of biofilm growth. Data generated by these two techniques is different and complementary, and in conjunction they constitute a highly effective monitoring method of biofilm growth.

Biomethane Potential From Sugarcane Straw in Veracruz, Mexico: Combined Liquid Hot Water Pretreatment and Enzymatic or Biological Hydrolysis

“…Analysing the BMP tests of the liquid fractions, we found that the BMP of the LHW and enzymatic hydrolysis (EH) pretreatments were close to the theoretical results (395 ml CH 4 /g COD R , according to Houbron et al, 2012) with 316.12 and 342.68 ml CH 4 /g COD R , respectively, Table 2. These results were higher than those previously reported by Rabelo et al, 2011 andJanke et al, 2017 who obtained a maximum BMP of 183-152 ml and 262.5 CH 4 /g COD R , respectively.…”

Tratamiento De Vinazas en Un Reactor De Lecho Fluidizado Inverso Anaerobio

“…Michaud et al (2005) y Houbron et al (2012) indican que el rendimiento de metano, definido como la cantidad de metano producido para una cantidad dada de materia orgánica removida, es el resultado de la actividad del consorcio anaerobio de bacterias. Esto es, el valor del rendimiento de metano es constante cuando el ecosistema anaerobio usa carbono para crecer y mantenerse solamente.…”

“…En relación con esto, muchos de los sistemas anaerobios empleados involucran sistemas de biomasa fija. La biopelícula proporciona interesantes ventajas tales como: ofrece una mayor superficie de contacto entre la materia orgánica y la biomasa, además de la fácil separación del agua tratada de la biomasa y de un diseño de sistema compacto (Houbron et al 2012, Thaiyalnayaki y Sowmeyan 2012.…”

Tratamiento de vinazas provenientes de etanol en un reactor de lecho fluidizado inverso

“…La biopelícula proporciona interesantes ventajas tales como: ofrece una mayor superficie de contacto entre la materia orgánica y la biomasa, la fácil separación del agua tratada de la biomasa y un diseño de sistema compacto (Houbron et al 2012, Thaiyalnayaki et al 2012). …”