Methanogen diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16S rDNA analysis

Diversity analysis of methanogens in rumen of Bubalus bubalis by 16S riboprinting and sequence analysis

“…Recent study carried out on rumen methanogens of Surti buffaloes furthermore supports the fact in which after phylogenetic analysis was done of 23 operational taxonomic units (OTUs) based on unique 16S rDNA sequences, they found 12 OTUs (52.17%) affiliated to Methanomicrobiales order. Results of Surti buffaloes also strengthen our results in Murrah buffaloes that Methanomicrobiales is the dominant order of methanogens present in our climatic and dietary conditions (Singh et al, 2011).…”

16S rDNA analysis of archaea indicates dominance of Methanobacterium and high abundance of Methanomassiliicoccaceae in rumen of Nili-Ravi buffalo

“…The facultative acetoclastic methylotrophs like Methanosarcinales were not detected in the present study. These groups were reported to be present in numerically negligible numbers [7] or none [6] in earlier studies involving other breeds of buffaloes. While reviewing rumen methanogens across the globe, Janssen and Kris [1], concluded that although Methanosarcina spp.…”

“…Several species of methanogens have been isolated from ruminants but major species of rumen methanogens are yet to be identified [1,5]. Only two studies had been reported on diversity of methanogens in buffaloes in India eone on Murrah breed [6] and the other on Surti breed [7]. These studies indicated substantial differences in methanogenic community between the two breeds and substantial differences with that reported in cattle [8].…”

Diversity of gut methanogens in herbivorous animals

“…For example, two studies conducted in India reported that the most abundant methanogens in the rumen of Murrah (Chaudhary and Sirohi, 2009) and Surti (Singh et al, 2012) breeds of water buffalo fed a concentrate : forage diet were 94.4% to 97.1% related to Methanomicrobium mobile, respectively. In contrast, a study conducted in Brazil on water buffaloes of the Mediterranean breed found that Methanobrevibacter-related phylotypes were the most abundant rumen methanogens at 91.5%, regardless of diet (grazing, corn silage and concentrate, or sugar cane and concentrate; Franzolin et al, 2012).…”