Methicillin Resistance in Staphylococcus Aureus: Mechanisms and Modulation

Stapleton, Paul; Taylor, Peter W.

doi:10.3184/003685002783238870

Cited by 340 publications

(259 citation statements)

References 53 publications

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“…Such an opportunistic bacterium is resistant to all beta-lactam antibiotics due to its ability to produce an additional penicillin binding protein (PBP) called PBP2a which has a low affinity for those antibiotics (Lim and Strynadka 2002;Selvey et al 2000;Stapleton and Taylor 2002).…”

Section: Introductionmentioning

confidence: 99%

Effects of passage number on growth and productivity of hybridoma secreting MRSA anti-PBP2a monoclonal antibodies

Corrêa

Senna²,

Sousa³

2015

Cytotechnology

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Monoclonal antibodies (mAb) are high added value glycoproteins recommended for immunotherapy, diagnosis, and also for the treatment of bacterial infections resistant to multiple drugs such as Methicillin Resistant Staphylococcus aureus (MRSA). In addition to environmental conditions related to cell cultures, the intrinsic characteristics of hybridoma cells, like the secretion stability of monoclonal antibodies by the cells through successive subcultures, are relevant for the characterization of cell lines related to the productivity of mAb. The rate of mAb production differs significantly between different cell lines and different passage numbers, and it is an important variable in characterization of cell lines. In order to find a more robust, fastergrowing, and higher-productivity cell line of hybridoma, cultivations in 24-well plates were performed in different subculture periods, or cell passages (P), of hybridoma cells producing MRSA anti-PBP2a monoclonal antibodies ]. The objective of this study was to study the effects of cell growth and production of MRSA-antiPBP2a mAb secreted by murine hybridoma cells grown in different passages as well as determine the which passages the hybridomas can be cultivated without harming their growth and productivity. So, cell growth profiles of hybridomas secreting MRSA-antiPBP2a (mAb) and the production of MRSA-antiPBP2a mAb in different subculture periods or cell passages (P) were studied. Cell growth tests, monoclonal antibody productivity, and metabolite characteristics revealed substantial differences in those cells kept between P10 and P50. Similarities in the secretion of monoclonal antibody, growth, and metabolic profiles, were noted in the MRSA-antiPBP2a mAb producing hybridoma cells kept between P10 and P20. Also, glucose consumption (g/L) and lactate production (g/L) in the latter cell cultures were monitored daily through biochemical analyzer. As of P30, it was observed a 4.4 times reduction in productivity, a 13 % reduction in metabolic yield, and a significant change in cell growth. Secretion of MRSA-antiPBP2a mAb should be obtained through the culture of hybridomas up to P20 in order to keep its stability.

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Section: Introductionmentioning

confidence: 99%

Effects of passage number on growth and productivity of hybridoma secreting MRSA anti-PBP2a monoclonal antibodies

Corrêa

Senna²,

Sousa³

2015

Cytotechnology

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“…EGCg has also been shown to be able to inhibit mammalian multidrug resistance P-glycoprotein (6). Furthermore, EGCg can act in synergy with penicillins and modulate the resistance of methicillin-resistant staphylococci (18).…”

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confidence: 99%

Epigallocatechin-Gallate Enhances the Activity of Tetracycline in Staphylococci by Inhibiting Its Efflux from Bacterial Cells

Roccaro¹,

Blanco²,

Giuliano³

et al. 2004

Antimicrob Agents Chemother

207

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Epigallocatechin-gallate (EGCg), the major catechin present in green tea extracts, has been shown to have several antibacterial activities, limiting bacterial growth and invasion and acting in synergy with ␤-lactam antibiotics. In this article, we report that EGCg at doses half and below its calculated MIC of 100 g/ml, is able to reverse tetracycline resistance in staphylococcal isolates expressing the specific efflux pump Tet(K) and appears to improve the MICs of tetracycline for susceptible staphylococcal isolates as well. The visible effect of EGCg is an increased accumulation of tetracycline inside bacterial cells. This effect is likely due to the inhibition of pump activity, and it is evident not only for Tet(K) pumps but also for efflux pumps of a different class [Tet(B)]. In summary, our data indicate that the observed dramatic enhancement by EGCg of tetracycline activity for resistant staphylococcal isolates is caused by impairment of tetracycline efflux pump activity and increased intracellular retention of the drug, suggesting a possible use of EGCg as an adjuvant in antibacterial therapy.

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“…Production of β -lactamase enzyme in the affected area is the main cause of microbial resistance. Some strain of MRSA are labelled as epidemic strains (EMRSA) [8]. Seventeen variants of EMRSA strain have been explored in the globe [9].…”

Section: Originationmentioning

confidence: 99%

Conquering methicillin resistant Staphylococcus aureus: perspectives and treatment options

Rashid

Qureshi

et al. 2015

Int Arch Med

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Exploring optimal cure of Methicillin Resistant Staphylococcus aureus (MRSA), has been consistently challenged by clinical physicians and microbiologist. The fatal bacterial infection, Methicillin Resistant Staphylococcus aureus (MRSA), shows unique resistance; when selected drugs are tested on human patients. If holistic approach is overlooked, less possibilities are left for medication to curtail this lethal toxicity. If single range of drug treatment is exercised only, the associated complications and consequences of infections may proliferate beyond threshold levels. The research article is an outcome from primary microbial data collection (from urine and blood) in hospital. The focus of experiment is to scrutinize the highest risk vulnerable hospital area including sampled fluid. The preventive and therapeutic measures are discussed along with multiple substitutes of medication with prudent selection to achieve targeted and optimum outcome.

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Methicillin Resistance in Staphylococcus Aureus: Mechanisms and Modulation

Cited by 340 publications

References 53 publications

Effects of passage number on growth and productivity of hybridoma secreting MRSA anti-PBP2a monoclonal antibodies

Effects of passage number on growth and productivity of hybridoma secreting MRSA anti-PBP2a monoclonal antibodies

Epigallocatechin-Gallate Enhances the Activity of Tetracycline in Staphylococci by Inhibiting Its Efflux from Bacterial Cells

Conquering methicillin resistant Staphylococcus aureus: perspectives and treatment options

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