Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan

Yasuda, Ryo; Kawano, Junichi; Onda, Humiaki; Takagi, Michihiro; Shimizu, Akira; Anzai, Toru

doi:10.2460/ajvr.2000.61.1451

Cited by 47 publications

(53 citation statements)

References 30 publications

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“…27 In addition, mecA-harboring, coagulase-negative (CoN) staphylococci have been isolated from several domesticated and healthy animals. 14,32 Potential human-to-animal transmission of MRSA has already been reported. 1 Studies in the United Kingdom concluded that MRSA is present in the general horse population and may represent a reservoir of new or rare MRSA strains that could be transmitted to humans.…”

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“…3 Furthermore, in Japan, mecA-positive CoN staphylococci were cultured from the skin and nares of healthy horses. 32,33 Recently, high prevalence of the CoN staphylococci was found in healthy horses in Europe. 4,28,26 However, no MRSA strains have been isolated.…”

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confidence: 99%

“…b Stock cultures were stored at 270uC in microbank vials c for further analysis. Bacterial DNA was used to detect mecA gene by polymerase chain reaction (PCR) technique 32 and analyzed by pulsed-field gel electrophoresis (PFGE). 5 The epidemiological relationship between isolates from horses and humans was assessed using described criteria.…”

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confidence: 99%

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Methicillin-Resistant Staphylococci Isolated from Healthy Horses and Horse Personnel in Italy

Martino¹,

Lucido

Mallardo

et al. 2010

J VET Diagn Invest

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Abstract. Methicillin-resistant staphylococci (MRS) were isolated from nasal swabs of 56 of 159 (35.2%; 95% confidence interval [CI]: 27.9-43.2%) healthy horses. Two nasal swabs were collected from each horse; 43 of 159 (27%; 95% CI: 20.5-34.8%) of the cohort were colonized by MRS strains in 1 nostril, while in the remaining 13 of 159 (8.2%; 95% CI: 4.6-13.9%), different or identical MRS strains were isolated in both nostrils. Of the 29 humans in close contact with the horses tested, 4 (13.8%; 95% CI: 4.5-32.6%) were found to be carriers of MRS. All isolates were coagulase negative with the exception of 2 coagulase-positive MRS strains, Staphylococcus aureus and Staphylococcus pseudintermedius, both isolated from horses. To assay the methicillin resistance, a susceptibility test to oxacillin with standardized disk diffusion method, a PBP-2a latex agglutination test, and a methicillin resistance gene (mecA) polymerase chain reaction assay were performed. Pulsed-field gel electrophoresis patterns of isolates from horses and humans in close contact with the horses revealed similarity. The results suggest evidence of transmission between animals, from animals to humans, and vice versa.

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Methicillin-Resistant Staphylococci Isolated from Healthy Horses and Horse Personnel in Italy

Martino¹,

Lucido

Mallardo

et al. 2010

J VET Diagn Invest

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“…As the isolate was susceptible to cephalosporins, the antimicrobial treatment did not play any role in selection pressure. In any case, though the origin of mecA is not clear now, the staphylococci harboring mecA was prevalent among not only horses [2,12,14] and chickens [8], but also pigs and cattle in Japan.…”

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In vitro Activity of 24 Antimicrobial Agents against Staphylococcus and Streptococcus Isolated from Diseased Animals in Japan

Morioka

Asai

Ishihara

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. A total of 88 Staphylococcus and 61 Streptococcus isolates from diseased animals throughout Japan were examined in 2000 for the minimum inhibitory concentrations of 24 different antimicrobials by the agar dilution method standardized by the Japanese Society of Chemotherapy. The resistance rates to aminobenzylpenicillin (36.4%) and benzylpenicillin (35.2%) were high in Staphylococcus isolates, and those to oxytetracycline (45.9%) and kanamycin (21.3%) were high in Streptococcus isolates. Two isolates resistant to oxacillin harbored the mecA gene. One was Staphylococcus epidermidis derived from a pig with arthritis, and the other Staphylococcus cohnii from a head of cattle with mastitis. KEY WORDS: antimicrobial susceptibility, Staphylococcus spp., Streptococcus spp.

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“…Selective media containing MPIPC which is incapable of inducing PBP-2' are believed less effective to MRSA of inducing type. For detection of MRSA from foods and animals, a selective medium containing 25 μg/ml CZX had been widely used [8,16,20], but CZX is no longer commercially available. There is a need to develop a new selective medium for effective screening for MRSA including inducible resistance type.…”

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Development of a New Selective Medium for Isolation of Methicillin-Resistant Staphylococcus aureus

Saito

Kakita

Shimoda

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. A new selective medium containing cephem antibiotics was developed for isolation of methicillin-resistant Staphylococcus aureus (MRSA). MRSA colonies on a medium containing ceftazidime (CAZ) were most easily identifiable and a medium containing cefoperazone (CPZ) was superior in suppressing the growth of other bacteria. With the medium containing a couple of CAZ and CPZ, MRSA and methicillin-resistant coagulase-negative staphylococci (MRCNS) were detected from 2 and 1 of 15 chicken meat samples respectively. The MRSA and MRCNS recovery test showed that the medium was effective for MRSA isolation, suppressing the growth of other bacteria efficiently. These results suggested that the medium containing a couple of CAZ and CPZ was useful for MRSA detection from foods and animals.

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Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan

Abstract: Methicillin-resistant coagulase-negative staphylococci that were highly resistant to various antibiotics were isolated from apparently healthy horses in Japan. These organisms must be considered a potential threat to horses and veterinarians who care for them.

Cited by 47 publications

References 30 publications

Methicillin-Resistant Staphylococci Isolated from Healthy Horses and Horse Personnel in Italy

Methicillin-Resistant Staphylococci Isolated from Healthy Horses and Horse Personnel in Italy

<i>In vitro</i> Activity of 24 Antimicrobial Agents against <i>Staphylococcus</i> and <i>Streptococcus</i> Isolated from Diseased Animals in Japan

Development of a New Selective Medium for Isolation of Methicillin-Resistant Staphylococcus aureus

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