Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

Abstract

Background: Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results: Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n = 5), ST398-t2330-IV (n = 1), ST398-t034-V (n = 1), ST225-t003-II (n = 1) and ST4894-t011-IV (n = 1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex spa-CC11. Conclusion: According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare -associated MRSA strains are still not spread among animals.

The Effect of Age and Sampling Site on the Outcome of Staphylococcus aureus Infection in a Rabbit (Oryctolagus cuniculus) Farm in Italy

“…As reported previously, S. aureus can be isolated from the whole body of rabbits: The nose, ear, skin of the interdigital spaces, the skin of the forelegs, the skin of the axillary and inguinal regions, the skin around the nipples, the perineum, the vagina, and the foreskin [10,[32][33][34][35][36]. The nose was considered the best choice here, due to the homogeneous distribution between categories.…”

Ecology and Genetic Lineages of Nasal Staphylococcus aureus and MRSA Carriage in Healthy Persons with or without Animal-Related Occupational Risks of Colonization: A Review of Global Reports

“…The pooled rate of MRSA-CC398 nasal carriage among healthy veterinary personnel (Group C) was of 2.6%, representing 28.4% of total MRSA of this group ( Figure 5 ). They were detected in 6 of the 11 studies in which molecular typing was performed, and they were carried out in Switzerland, the Czech Republic, and the UK [ 121 , 122 , 123 , 124 , 135 , 136 ]. Most of the isolates corresponded to the spa type t011, but in a few cases, t034 or t899 were also identified.…”

“…The few genomic studies of S. pseudintermedius have offered a glimpse of its diversity, epidemiological characteristics, and antimicrobial resistance gene profile [ 185 , 212 ]. Among them, S. pseudintermedius was reported from studies by Paul et al [ 138 ], Boost et al [ 139 ], and Neradova et al [ 135 ] with a prevalence of 3.9, 0.7, and 1.5%, respectively ( Supplementary Table S7 ). However, only a study by Boost et al [ 139 ] performed the molecular typing of the S. pseudintermedius isolate.…”

“…No CC398-MRSA was reported in Group B. Citations for Figure 8 a: China [ 49 ], Austria [ 62 ], Belgium [ 62 ], Croatia [ 62 ], Netherlands [ 62 ], Germany [ 62 ], Switzerland [ 121 , 122 , 123 ], Denmark [ 124 ], Czech Republic [ 135 ], UK [ 136 ]. Citations for Figure 8 b: Poland [ 125 ], Korea [ 152 ], China [ 155 ], Switzerland [ 121 , 168 ], Italy [ 161 , 167 ], Germany [ 172 , 174 , 175 ], Spain [ 181 ], Netherlands [ 181 ], USA [ 184 ], Canada [ 186 ].…”

Antibiotic Resistance, spa Typing and Clonal Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Blood of Patients Hospitalized in the Czech Republic

“…These spa types were detected in more than 90% of the spa-typed MRSA strains from the Czech livestock animals (pigs, cattle, goats and sheep) as well as from food of animal origin and the environment [27]. A recent study [28] investigated nasal MRSA carriage among veterinary professionals from the Czech Republic. The majority of isolates belonged to ST398 and were clustered into spa CC011.…”