Methicillin-resistant Staphylococcus aureus (MRSA) associated with mastitis among water buffaloes in the Philippines

Badua, Alona T.; Boonyayatra, Sukolrat; Awaiwanont, Nattakarn; Gaban, Paula Blanca V.; Mingala, Claro N.

doi:10.1016/j.heliyon.2020.e05663

Cited by 12 publications

(12 citation statements)

References 31 publications

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“…The prevalence of methicillin-resistant S. aureus (MRSA) in buffalo dairy farms and raw milk was recently investigated by Normanno et al (2020) [ 38 ] in Italy and Badua et al (2020) [ 39 ] in the Philippines. Such prevalence of MRSA detected and characterized from buffalo and bovine milk samples was earlier reported worldwide, bringing out rates between 15% of isolated S. aureus (in China) [ 40 ], 41% (in Turkey) [ 41 ], and 61% (in the Philippines) [ 39 ]. The outcomes of the present study, 37.5% of isolated coagulase-positive Staphylococci ( Table 5 ), can be placed within the mean values of available studies.…”

Section: Resultsmentioning

confidence: 99%

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Carraturo

Alterisio

Guccione

et al. 2022

IJERPH

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The research was focused on the surveillance of the exposure of buffalo milkers in contact with both animals and potentially contaminated equipment, pointing attention on the diffusion of antibiotic-resistant Staphylococcus spp. The monitoring was performed for 12 months, allowing the collection of 600 raw milk and buffalo udder surface samples, 192 milking lanes, 400 milking clusters, 160 personal protective equipment (PPEs) and electronic devices surface samples in contact with the workers of four milking parlors located in Southern Italy. The analysis of the milk samples evidenced the highest exposure to the bacteria considered (and mainly to S. aureus) from late winter–spring seasons onward. The possible risk arising from buffalo udder, milking clusters, and lines were instead considered rather stable along the entire period of sampling. The PPEs turned out to be a source of contamination for milkers mainly during the spring and summer periods. The analysis for oxacillin/methicillin resistance revealed in all the farms enrolled an overall amount of 37.5% of Staphylococci strains (belonging to S. aureus, S. haemolyticus, S. pseudintermedius, S. chromogenes species) resistant both to methicillin and oxacillin. The investigation demonstrated that the potential transfer of pathogenic bacteria to humans would have a better chance to occur at milk resumption time (since late winter–spring onward) when the number of animals to be milked is greater and the activity in the milking parlor is more challenging. At the same time, the findings seem to point out that the potential risk may be worsened by a significant presence of oxacillin/methicillin-resistant Staphylococci, potentially resulting from irrational use of antibiotics.

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Section: Resultsmentioning

confidence: 99%

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Carraturo

Alterisio

Guccione

et al. 2022

IJERPH

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“…These drugs are commonly used in veterinary medicine in Indonesia [ 21 ]. Staphylococcus aureus is also considered the primary cause of mastitis in cattle, water buffaloes, and goats in Asia [ 11 , 18 , 21 ]. In Egypt [ 24 ], 100% of S. aureus isolates were resistant to AMP and OX from 77 isolates of cow and buffalo milk suspected of MRSA and 84.8% of S. aureus were resistant to AMP and 74.1% to OX from 112 isolates of cow and buffalo milk.…”

Section: Discussionmentioning

confidence: 99%

“…Most MRSA isolates are still susceptible to common antibiotics. Nevertheless, ~37.5% of the isolates were considered MDR [11]. The emergence of MRSA in livestock has been implicated in the use of antimicrobials as growth promoters and for preventive and therapeutic measures [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus isolates derived from humans and animals in Yogyakarta, Indonesia

et al. 2023

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Background and Aim: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain in veterinary and human medicine is a growing global problem. This study aimed to evaluate MRSA isolates of human and animal origin against various antibiotics in Yogyakarta, Indonesia. Materials and Methods: The susceptibility test was carried out by the disk diffusion method using Mueller-Hinton agar against nine antibiotic disks. Methicillin-resistant S. aureus strains were genetically confirmed through mecA gene detection encoding for methicillin resistance by polymerase chain reaction. Results: All 240 S. aureus strains isolated from animals and humans were resistant to penicillin G (P) (100% and 99%, respectively), followed by ampicillin (AMP), amoxicillin (AML), oxacillin (OX), erythromycin (E), clindamycin (DA), tetracycline (TE), gentamicin (GEN), and ciprofloxacin (CIP). Eighty-three MRSA strains were resistant to OX (100%), P (100%), AMP (99.27%), AML (95.52%), E (87.77%), TE (71.33%), DA (63.24%), GEN (38.81%), and CIP (26.87%). Conclusion: The antimicrobial resistance pattern of S. aureus human isolates was similar to their animal counterpart, with 77.20% of MRSA strains classified as multidrug-resistant (MDR) bacteria. These findings indicate an increase in MDR S. aureus strains of animal origin in Yogyakarta, thus raising public health concerns about MRSA zoonotic spread.

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“…In J&K also, the major mastitis-causing bacteria (66.67%) have been found to be S. aureus [7]. Recently, different studies have found that S. aureus shows resistance against most commonly used antibiotics, and the possible explanation put forward by researchers includes confinement to extracellular space and the long replication period within polymorph nuclear leukocytes referred to as dormant bacteria [8,9]. Furthermore, for effective resolution of mastitis, we need to use high-end techniques and technologies and protocols which include bacterial isolation and antibiotic sensitivity testing so that effective antibiotic can be selected against mastitis [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Therapeutic Efficacy of Copper Nanoparticles in Staphylococcus aureus‐Induced Rat Mastitis Model

Taifa

Muhee

Bhat

et al. 2022

Journal of Nanomaterials

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The current study was devised to develop alternative, nonantibiotic, and economically viable treatments for bovine mastitis. The concentration of 6.25 μg/mL (25 nm) CuNPs was selected as intramammary (IM) treatment in S. aureus-induced mastitis in rats as this concentration showed a significant zone of inhibition through the in vitro sensitivity test and minimal cell toxicity on fibroblast cell lines. After, this in vivo study was conducted, and rats were divided into four groups of 6 rats each: group I (healthy control/deionized water), group II (disease control), group III (CuNPs), and group IV (gentamicin). Injection of gentamicin IM for 5 days was selected on the basis of an antibiotic sensitivity test. The therapeutic efficacy of CuNPs was assessed on the basis of clinical signs, mammary gland architecture, bacterial load, oxidative stress parameters, and histopathology of mammary glands. The clinical signs of mastitis in group III ameliorated within 3 days of treatment while in group IV clinical signs ameliorated within 4 days of initiation of treatment. On the 5th day after randomization, bacterial load, mammary gland weights, TOS (Total Oxidant Status), and OSI (oxidative stress index) were significantly lower in the CuNPs group compared to the disease control group and commercial antibiotic group. Similarly, TAS of group III was significantly higher compared to that of groups II and IV indicating that CuNPs have better ameliorative efficacy in mastitis. Treatment with IM, CuNPs @ 6.25 μg/mL showed early recovery, reduced bacterial loads, and amelioration of oxidative stress indices henceforth resulting in marked amelioration in histopathological changes compared to rats in group IV. From this study, it may be concluded that CuNPs may provide a potential alternative therapeutic regimen for the treatment of bovine mastitis.

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Methicillin-resistant Staphylococcus aureus (MRSA) associated with mastitis among water buffaloes in the Philippines

Cited by 12 publications

References 31 publications

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Exposure of Buffalo Milkers to Pathogenic Bacteria and Characterization of Isolated Methicillin-Resistant Staphylococcus spp.

Methicillin-resistant Staphylococcus aureus isolates derived from humans and animals in Yogyakarta, Indonesia

Evaluation of Therapeutic Efficacy of Copper Nanoparticles in Staphylococcus aureus‐Induced Rat Mastitis Model

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