Methicillin-resistant Staphylococcus pseudintermedius isolated from canine pyoderma in North China

Wang, Y.; Yang, Jing; Liu, K.; Cao, Xingyuan; Zhang, W.; Shen, Jianzhong; Wu, Congming

doi:10.1111/j.1365-2672.2012.05233.x

Cited by 41 publications

(35 citation statements)

References 33 publications

(74 reference statements)

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“…According to a recent report, the prevalence of MRSP in North America and Canada is 0%~7% [26]. These rates are slightly elevated in other countries with 12.7% in China [29], 11.4% in Japan [16], and 17.6% in Korea [31]. In the present investigation, the MRSP rate was 34%, which was higher than that previously reported in studies of skin.…”

Section: Discussioncontrasting

confidence: 63%

Antibiotic resistance and molecular characterization of ophthalmicStaphylococcus pseudintermediusisolates from dogs

et al. 2014

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The prevalence, virulence potential, and antibiotic resistance of ophthalmic Staphylococcus pseudintermedius (SP) isolated from dogs were examined. Sixty-seven Staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the Staphylococcus intermedius group (SIG) nuclease gene (SInuc), exfoliative toxin gene for SIG (siet), and antibiotic resistance genes (blaZ and mecA). PCR-restriction fragment length polymorphism analysis of the pta gene was also performed. Fifty isolates were identified as SIG strains, all of which were found to be SP. The blaZ gene was detected in 42 of the 50 SP strains and mecA gene was observed in 18 of the 50 SP strains. The 50 SP strains were most susceptible to amoxicillin/clavulanic acid (94%) and chlorampenicol (70%), and highly resistant to tetracycline (94%) and penicillin (92%). It was also found that 16 (88.9%) mecA-positive SP strains were resistant to oxacillin, tetracycline and penicillin. All mecA-positive SP were resistant to more than four of the eight tested antibiotics and therefore considered SP with multi-drug resistance (MDR). Our results indicate a high prevalence of antibiotic resistance genes in ophthalmic SP along with a close relationship between MDR SP strains and the mecA gene. Based on our findings, judicious administration of antibiotics to companion dogs is necessary.

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Section: Discussioncontrasting

confidence: 63%

Antibiotic resistance and molecular characterization of ophthalmicStaphylococcus pseudintermediusisolates from dogs

et al. 2014

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“…Therefore, the prevalence might be lower in that study due to the absence of pyoderma (healthy animals). On the other hand, similar prevalences were found in China (92%) (Wang et al 2012) and Brazil (96%) (Bourguignon 2012). Both brazilian and chinese groups used samples from animals with pyoderma, thus reinforcing the idea that presence of the disease increases the presence S. pseudintermedius.…”

Section: Discussionmentioning

confidence: 61%

“…Such resistant strains are called methicillin-resistant Staphylococcus pseudintermedius (MRSP) (Weese & Van Duijkeren 2010). The MRSP strains carry the mecA gene, which encodes an altered penicillin-binding protein known as PBP2a, which reduces the bacterial affinity to all beta-lactamic antibiotics (Weese & Van Duijkeren 2010, Wang et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma

et al. 2016

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RESUMO.-[Prevalência e suscetibilidade in vitro deIn order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs. observada entre a presença do gene mecA nas lesões de pele e nas narinas. Sendo assim, resistência à oxacilina in vitro pode ser aferida com segurança para indicar a presença do gene mecA em amostras de MRSP, e as narinas podem constituir em um reservatório dos microorganismos em cães.

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“…8,13,19 SIG includes 3 distinct species: Staphylococcus intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. 1,14 The newest described species, S. pseudintermedius, is the most common cause of canine staphylococcal infections.…”

Section: Brief Communicationmentioning

confidence: 99%

An evaluation of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification ofStaphylococcus pseudintermediusisolates from canine infections

et al. 2015

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Abstract. It has been proposed, based on taxonomic and molecular studies, that all canine isolates belonging to Staphylococcus intermedius group (SIG) should be renamed Staphylococcus pseudintermedius. However, isolates of SIG and other coagulase-positive staphylococci share many phenotypic characteristics, which could lead to misidentification. The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying S. pseudintermedius isolates obtained from canine infections was evaluated, using a polymerase chain reaction (PCR)-based identification as the gold standard. In addition, MALDI-TOF MS was compared with conventional biochemical tests. A central problem was the incorrect identification of S. pseudintermedius isolates as S. intermedius by either MALDI-TOF MS or biochemical identification. From the 49 S. pseudintermedius isolates identified by the molecular method, only 21 could be assigned to this species by the biochemical approach and only 12 by MALDI-TOF MS. The 6 S. aureus isolates were correctly identified by all 3 techniques. However, using biochemical tests, 9 S. pseudintermedius were mistakenly classified as S. aureus, indicating a reduced specificity relative to the MALDI-TOF MS system. Analysis with the MALDI-TOF MS platform allowed rapid and accurate identification of the 49 isolates to the S. intermedius group but the approach was very limited in identifying S. pseudintermedius isolates, as only 12 of 49 isolates were correctly identified, a sensitivity of 0.24 (95% confidence interval: 0.13-0.39).

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Methicillin-resistant Staphylococcus pseudintermedius isolated from canine pyoderma in North China

Cited by 41 publications

References 33 publications

Antibiotic resistance and molecular characterization of ophthalmicStaphylococcus pseudintermediusisolates from dogs

Antibiotic resistance and molecular characterization of ophthalmicStaphylococcus pseudintermediusisolates from dogs

Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma

An evaluation of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification ofStaphylococcus pseudintermediusisolates from canine infections

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