Methionine supplementation improves ram sperm parameters during liquid storage at 5 °C

Bucak, Mustafa Numan; Çoyan, Kenan; Öztürk, Caner; Güngör, Salim; Ömür, Ali Doğan

doi:10.1016/j.cryobiol.2012.09.002

Cited by 18 publications

(12 citation statements)

References 13 publications

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“…When other antioxidants have been tested in liquid ram sperm, contradictory results have been obtained, suggesting that results could be influenced by other factors such as the male, extender, antioxidant or concentration (Silva, Cajueiro, Silva, Soares, & Guerra, 2012). Assorted results are found in literature showing positive (using catalase, methionine, astaxanthin or vitamin C) (Bucak, Coyan, Oztürk, Güngor, & Omür, 2012;Câmara et al, 2011;Fang et al, 2015) or not positive effects (such as glutathione, ergothioneine, reduced glutathione or trolox) (Bucak & Tekin, 2007;Mata-Campuzano et al, 2014) of antioxidants on the sperm motility. In this sense, some authors suggested that the addition of natural substances having antioxidant effect acts as double-edged swords, since high exogenous antioxidants may disrupt redox balance and acts like a pro-oxidant by activating pathways such as increasing pro-inflammatory mediators production, nitrosylation of proteins, proglycation effect-and endocrine-disrupting activities (Bouayed & Bohn, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effect of olive‐derived antioxidants (3,4‐dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

Arbulu

Delgado

Bermúdez-Oria

et al. 2020

Reprod Domestic Animals

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The aim of the present study was to evaluate the effect of the addition of two olive oil‐derived antioxidants, hydroxytyrosol (3,4‐dihydroxyphenylethanol, HT) and 3,4‐dihydroxyphenylglycol (DHPG), on ovine semen during liquid storage at 5°C and 15°C. Semen was collected, pooled, diluted and then divided into aliquots supplemented with different concentrations (5 μg/ml, 10 μg/ml, 50 μg/ml and 100 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants. Sperm motility characteristics were assessed in the different samples at 0, 6, 24, 48, 72 and 96 hr after cooling, and a fertility trial was also conducted. The results showed that the antioxidant addition did not significantly improve total and progressive motility in ovine cooled sperm maintained at 15° or 5°C. However, in samples stored at 5°C, LIN (48, 72, 96 hr), STR (0 hr) and WOB (0, 48, 72, 96 hr) values significantly decreased in comparison with control treatment when high antioxidant concentrations were added (MIX100 or HT100). When samples were maintained at 15°C, MIX50 showed significantly higher VCL values than the control treatment after 6 hr cooling, and MIX100 showed significantly lower VCL values at 96 hr after cooling. According to the artificial insemination trial, no significant differences were observed when antioxidants were added. In conclusion, the use of HT and DHPG showed small impact in sperm motility and fertility was not affected (nor detrimentally nor positively) when insemination was carried out using antioxidant‐supplemented liquid sperm.

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Section: Discussionmentioning

confidence: 99%

Effect of olive‐derived antioxidants (3,4‐dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

Arbulu

Delgado

Bermúdez-Oria

et al. 2020

Reprod Domestic Animals

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“…It is possible that many factors affect the sperm physiology during storage, and this scenario offers an important opportunity to test protective compounds such as antioxidants. With previous research, it has been suggested that adding antioxidants -either enzymatic (Maxwell and Stojanov, 1996) or non-enzymatic (Bucak et al, 2012;Mara et al, 2005) -could benefit the quality and fertility of ram semen after liquid storage at different temperatures. Therefore, a study was previously performed on the effects of the antioxidants Trolox and reduced glutathione (GSH) during cooled storage at 5 • C and 15 • C. Trolox, an analog of vitamin E, has been successfully tested for controlling lipid peroxidation in ram sperm during cryopreservation (Maia et al, 2010), and thiols have been proved to be excellent radical scavengers and blockers of lipid peroxidation (Deneke, 2000).…”

Section: Introductionmentioning

confidence: 99%

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Mata-Campuzano

Álvarez‐Rodríguez

Tamayo-Canul

et al. 2014

Animal Reproduction Science

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Antioxidants have a potential to improve the quality and fertility of refrigerated-stored ram semen. Reduced glutathione (GSH) and Trolox (0.2, 1 and 5mM) were evaluated in ram semen preserved at 15 and 5°C up to 48 and 96h, respectively. Extenders were also evaluated (15°C: Tris-citrate-fructose, TCF, without lipids, and TES-Tris-fructose 10% egg yolk, TTF-EY; 5°C: TTF-EY and 3.5% soybean lecithin, TTF-SL; INRA96 at both temperatures). Storage at 5°C resulted in poorer quality than 15°C up to 48h, while allowing acceptable quality at 96h. Antioxidants had few effects on sperm quality, with use of Trolox resulting in reduced motility and viability in TCF. Storage at 15°C in the TCF extender resulted in decreased motility, viability and mitochondrial activity compared with use of TTF-EY. Sperm quality when storage was at 5°C was similar, but storage in TTF-SL resulted in decreased motility and mitochondrial activity. Acrosomal status was only slightly affected by extender and antioxidant. Mitochondrial activity was improved by antioxidants in TTF-SL, and GSH at 5mM when semen was stored at 5°C in TTF-EY. A preliminary artificial insemination trial indicated that supplementation with GSH has the potential for improving lambing (P<0.1). In conclusion, use of antioxidants resulted in lesser effects than extender composition or storage time on quality of ram semen. Use of Trolox negatively impacted sperm quality and GSH had some positive impacts. The use of soybean lecithin requires further research to assess its impact on mitochondria.

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“…Consequently, it can be speculated that the supplementation of extender with 100-200 lg/ml silymarin improves ram sperm longevity under liquid storage. It was reported that using other antioxidants such as resveratrol, quercetin and methionine was improved sperm quality rate (Sarl os et al 2002;Coyan et al 2010;Bucak et al 2012;Silva et al 2012). Accumulation of the end products of metabolism, which include cytotoxic aldehydes, induces more ROS generation and lipid peroxidation during sperm storage (Aitken et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form

Mehr

Parisoush

2016

Reprod Domestic Animals

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Two experiments were designed to evaluate the effect of silymarin on stored spermatozoa using four rams. In experiment 1, silymarin was evaluated as a supplement for Tris-glucose extender. Semen samples (n = 20) were diluted with extender containing 0, 50, 100, 150 and 200 μg/ml silymarin and incubated at 5°C for 72 h. Membrane integrity, acrosome integrity, sperm viability and motility were evaluated at 72 h. Concentration of malondialdehyde (MDA) was determined after 48 h. Membrane integrity was higher in 100 μg/ml silymarin (65.2%) than control group (43.2%, p < 0.05). Acrosome integrity was highest in 100 μg/ml silymarin (71.3%, p < 0.05). Progressive motility was higher in 100 (58.5%), 150 (60.62%) and 200 μg/ml silymarin (54.7%) than control group (30.7%, p < 0.05). The highest MDA concentration was observed in control group (400 mm/10 × 10(6) sperm; p < 0.05). The goal of experiment 2 was to determine the interaction between silymarin and caproic acid on ram stored sperm. Ejaculates (n = 20) were diluted by Tris-glucose extender, added 0 (S- ) or 100 μg/ml (S+ ) silymarin and 0 (C- ) or 0.3125% (C+ ) caproic acid, and thereafter, aliquots were incubated at 5°C for 72 h. Membrane integrity was lower in C- S- (57.6%) than C- S+ (73.2%), C+ S- (80.2%) and C+ S+ (72.1%, p > 0.05). The highest sperm viability and acrosome integrity were observed in C+ S- (82.4 and 80.1%, respectively; p < 0.05). There was no difference between C- S+ and C+ S+ on sperm viability and membrane integrity, progressive motility and MDA concentration (p > 0.05). Therefore, the supplementation of extender with silymarin and caproic acid improved sperm quality and caproic acid was superior to caproic acid plus silymarin.

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Methionine supplementation improves ram sperm parameters during liquid storage at 5 °C

Cited by 18 publications

References 13 publications

Effect of olive‐derived antioxidants (3,4‐dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

Effect of olive‐derived antioxidants (3,4‐dihydroxyphenylethanol and 3,4 dihydroxyphenylglycol) on sperm motility and fertility in liquid ram sperm stored at 15°C or 5°C

Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage time

Effect of Different Levels of Silymarin and Caproic Acid on Storage of Ram Semen in Liquid Form

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