Method Development and Validation of Six Bile Acids for Regulated Bioanalysis: Improving Selectivity and Sensitivity

Voelker, Troy; Wang, Hongxia; Irish, Mitzi; Wang, Juan; Harrison, S. D.; Reuschel, Scott; Bennett, Patrick; Mei, Meng

doi:10.4155/bio.13.94

Cited by 16 publications

(4 citation statements)

References 24 publications

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“…The measurement of bile acids was performed similarly as previously described (Voelker et al, 2013) with some modifications. Briefly, concentrations of primary and secondary bile acids in human plasma were quantified using a Shimadzu CBM-20A HPLC system coupled with a Thermo Q Exactive Focus mass spectrometer.…”

Section: Measurement Of Bile Acids By Liquid Chromatography-mass Spectrometry (Lc-ms)mentioning

confidence: 99%

Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans

Hagan

Cortese

Rouphael

et al. 2019

Cell

474

406

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Emerging evidence indicates a central role for the microbiome in immunity. However, causal evidence in humans is sparse. Here, we administered broad-spectrum antibiotics to healthy adults prior and subsequent to seasonal influenza vaccination. Despite a 10,000-fold reduction in gut bacterial load and long-lasting diminution in bacterial diversity, antibody responses were not significantly affected. However, in a second trial of subjects with low pre-existing antibody titers, there was significant impairment in H1N1-specific neutralization and binding IgG1 and IgA responses. In addition, in both studies antibiotics treatment resulted in (1) enhanced inflammatory signatures (including AP-1/NR4A expression), observed previously in the elderly, and increased dendritic cell activation;(2) divergent metabolic trajectories, with a 1,000-fold reduction in serum secondary bile acids, which was highly correlated with AP-1/NR4A signaling and inflammasome activation. Multiomics integration revealed significant associations between bacterial species and metabolic phenotypes, highlighting a key role for the microbiome in modulating human immunity.

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Section: Measurement Of Bile Acids By Liquid Chromatography-mass Spectrometry (Lc-ms)mentioning

confidence: 99%

Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans

Hagan

Cortese

Rouphael

et al. 2019

Cell

474

406

View full text Add to dashboard Cite

show abstract

“…A high resolution MS (HRMS) detection was introduced by Voelker et al. [105] as a complementary alternative to a traditional HPLC‐MS/MS method using triple quadrupole. HRMS overcomes its limitations caused by the analyte fragmentation, higher background noise, or relatively narrow mass range.…”

Section: Biological Samplesmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

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“…It was a unanimous consensus that in HRMS, sensitivity is improved because of reduced background noise, which improves the signal-to-noise ratio so that a low LLOQ can be achieved even for very complex matrices [11]. Sensitivity is also gained for molecules found to have poor MRM fragmentation on standard triple quadrupole mass spectrometers.…”

Section: Discussion Topicsmentioning

confidence: 99%

2015 White Paper on Recent Issues in Bioanalysis: Focus on New Technologies and Biomarkers (Part 1 – Small Molecules by LCMS)

Welink

Fluhler

Hughes³

et al. 2015

Bioanalysis

Self Cite

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The 2015 9th Workshop on Recent Issues in Bioanalysis (9th WRIB) took place in Miami, Florida with participation of over 600 professionals from pharmaceutical and biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. It is once again a 5-day week long event - a full immersion bioanalytical week - specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest in bioanalysis. The topics covered included both small and large molecules, and involved LCMS, hybrid LBA/LCMS, LBA approaches including the focus on biomarkers and immunogenicity. This 2015 White Paper encompasses recommendations that emerged from the extensive discussions held during the workshop, and is aimed to provide the bioanalytical community with key information and practical solutions on topics and issues addressed, in an effort to advance scientific excellence, improve quality and deliver better regulatory compliance. Due to its length, the 2015 edition of this comprehensive White Paper has been divided into three parts. Part 1 covers the recommendations for small molecule bioanalysis using LCMS. Part 2 (hybrid LBA/LCMS and regulatory agencies' inputs) and Part 3 (large molecule bioanalysis using LBA, biomarkers and immunogenicity) will also be published in volume 7 of Bioanalysis, issues 23 and 24, respectively.

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Method Development and Validation of Six Bile Acids for Regulated Bioanalysis: Improving Selectivity and Sensitivity

Abstract: This is the first LC-high-resolution/accurate mass assay fully validated to quantify six bile acids for regulated bioanalysis.

Cited by 16 publications

References 24 publications

Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans

Antibiotics-Driven Gut Microbiome Perturbation Alters Immunity to Vaccines in Humans

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2015 White Paper on Recent Issues in Bioanalysis: Focus on New Technologies and Biomarkers (Part 1 – Small Molecules by LCMS)

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