Method Development and Validation of Metformine, Pioglitazone and Glibenclamide in Tablet Dosage Form by using RP-HPLC

Tengli, Anandkimar R.

doi:10.4172/2161-1009.1000130

Cited by 4 publications

(6 citation statements)

References 18 publications

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“…Zero-order model: A = K0t+A0 …………. (1) First-order model: Log A = Kt/2.3+Log A0 ……… (2) Higuchi-diffusion model: Q = KHt 1/2 ……… 3Korsmeyer-Peppas model: Ct/C∞ = Kt n …….. 4Hixson-Crowell diffusion release model: Q t/Q = Kt n ……. (5)…”

Section: Kinetic Analysis Of Glibenclamide In Vitro Releasementioning

confidence: 99%

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Formulation and Evaluation of Glibenclamide Gel for Transdermal Drug Delivery

ANWAR

Jan

GUL

2020

Int J Curr Pharm Sci

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Objective: The purpose of the recent study was to formulate glibenclamide gels for transdermal drug release, and to evaluate the oleic acid effect on the release of the preparations. Methods: Oleic acid was used at a range of concentrations in the gel formulations and its effects observed in Glibenclamide gel using In vitro release of drug was done in Franz diffusion cells, whereas pH 7.4 Phosphate buffer was used for release studies. Formulations were characterized by clarity, pH, homogeneity, viscosity, spreadabilty, skin irritation, drug content, stability studies. Scanning calroimetry analysis (SCA) and XRD studies were performed to assess the physical and chemical interactions. Results: Release profiles in vitro were observed. The released quantity of drug recovered from the glibenclamide gel after the addition of 1% oleic acid, increased with increasing concentration of the enhancer that is oleic acid. Whereas drug quantity recovered in the receptor solvent was 69.999% of Glibenclamide gel having 3% oleic acid. All the formulation were physicochemically stable. The data was statistically analyzed by using SPSS and DD solver. Conclusion: The drug is released and the oleic acid does enhance the release of the drug with the increase in its concentration.

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Section: Kinetic Analysis Of Glibenclamide In Vitro Releasementioning

confidence: 99%

“…It is more potent than first generation sulphonylurea. GLB is also called as glyburide and its formula is C23H28CIN3O5S [1].…”

Section: Introductionmentioning

confidence: 99%

Formulation and Evaluation of Glibenclamide Gel for Transdermal Drug Delivery

ANWAR

Jan

GUL

2020

Int J Curr Pharm Sci

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“…[3] These are organic compound containing two N-linked guanidines. Standard & sample preparation for UV-spectrophotometer analysis [4] : 25 mg of Metformin standard was transferred into 25 ml volumetric flask, dissolved & make up to volume with mobile phase.…”

Section: Metforminmentioning

confidence: 99%

Method Development and Validation of Metformin by Using Reverse- Phase High Performance Liquid Chromatography

Sahithi¹

2017

WJPR

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The method development and validation of Metformin based on UltraViolet spectrophotometry. This compound belongs to the class of organic compounds known as biguanides. These are organic compound containing two N-linked guanidines. Metformin is an oral anti diabetic drug in preventing complications of type 2 diabetes and it is a good first line therapy for a over obese with type 2 diabetes, it is currently available in more than 60countries worldwide. As a result of the importance of this oral hypoglycaemic agent in the treatment of non insulin dependent diabetes mellitus. The reported methods include the chromatographic methods such as HPLC. HPLC has been reported for the determination of Metformin chromatographic methods, these are complex, tedious, time consuming and are not suitable for routine analysis. However, Ultraviolet spectrophotometric methods which are simple and fast for ideal and routine analysis. We observed the maximum absorbance at 235&274nm for Metformin solution ELICO SL-159 make Ultra-Violet visible spectrophotometry spectrum model UV-2450, has been recorded when scanned in the ultraviolet region, using mobile aceto nitrile: phosphate buffer (30:70) with a flow rate of 1.0ml/minute and wave length at 225 nm. Thus, it was selected as the analytical wavelength. The development method was found to be simple, sensitive, accurate, precise and specific as evidenced by non-interference from the excipients presented in the marketed injection.

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“…The chemical name of glibenclamide is 5-Chloro-N-(2-{4-[(cyclohexylcarbamoyl) sulfamoyl] phenyl} ethyl)-2-methoxybenzamide and chemical formula is C 23 H 28 ClN 3 O 5 S (Figure 1). Therapeutically, it is more potent as compared to that of the first generation of sulphonylureas [1].…”

Section: Introductionmentioning

confidence: 99%

“…Method development and Validation for the analysis of active pharmaceutical ingredients in pharmaceutical dosage forms is very important as it gives the precision and accuracy of the developed method [1,[6][7][8]. Till now, various methods have been developed and validated for the analysis of various anti-diabetic agents [9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Analytical Method for Qualitative and Quantitative Determination of Glibenclamide in Different Brands of Tablet Dosage form Using UV-Visible Spectroscopy

Bilal¹

2013

J Mol Genet Med

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This paper describes a methodology to develop a new method for qualitative and quantitative determination of glibenclamide (GLB) in three brands of GLB tablets. We validated the developed method for linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) according to the guidelines of the International Conference on Harmonization. Finally, we estimated the qualitative and quantitative amount of GLB in three brands of GLB tablets using validated method. A non significant difference was observed in the dissolution profiles of GLB in these tablets. Validation of developed method showed that a linear relationship (r 2 >0.999) was observed at maximum absorbance (λ max ) at 229.5 nm with concentration range of 3-15 μg/ml GLB. Accuracy of developed method was found to be within the limit of 95-105%. The percent relative standard deviation (%RSD) and percent relative error (%RE) for precision was found to be <3%. In three brands of GLB tablets, values of LOD and LOQ were 10 ng/ml and 35 ng/ml respectively. We also found that the amount of GLB in each tablet corresponded to the requirements of 95-105% of the label claimed in tablet. From the results of validation of developed method, it concluded that developed method showed satisfactory linearity, precision and accuracy for analysis of active ingredients of commercially available pharmaceutical products.

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Method Development and Validation of Metformine, Pioglitazone and Glibenclamide in Tablet Dosage Form by using RP-HPLC

Cited by 4 publications

References 18 publications

Formulation and Evaluation of Glibenclamide Gel for Transdermal Drug Delivery

Formulation and Evaluation of Glibenclamide Gel for Transdermal Drug Delivery

Method Development and Validation of Metformin by Using Reverse- Phase High Performance Liquid Chromatography

Development and Validation of Analytical Method for Qualitative and Quantitative Determination of Glibenclamide in Different Brands of Tablet Dosage form Using UV-Visible Spectroscopy

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