Method for generation of peptide‐specific igy antibodies directed to Staphylococcus aureus extracellular fibrinogen binding protein epitope

Abstract

The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococcus aureus fibrinogen binding protein (Efb) and its peptidyl epitope (spanning residues 127-140). The Efb is an extracellular, adhesion protein which binds both human fibrinogen and complement C3 protein thus contributing to the high infectious potential of this pathogen. The selected epitope of Efb protein is responsible for the interaction with C3. The immunochemical characterization of both anti-Efb and epitope-specific IgY antibodies revealed their similar avidity, titer, and reactivity profile, although some differences in the hen's immune response to administered antigens is discussed.

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

“…Anti-Efb and anti-Efb 127-140 antibodies presented high titers in ELISA and strong avidity in Western blot, showing promising use in the diagnosis of S. aureus infection [110].…”

Evaluation of Human Anti Igg Polyclonal Antibody Production Conjugated with Peroxidase in Egg Yolk

“…The IgG antigen, with few minor changes, was emulsified along with complete Freund adjuvant (Merck, Germany) (20). Samples (consisting of eggs and hens blood) were collected from the experimental group egg-laying hens from week 0 (before immunization) until week 10 after immunization (21).…”

Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus

“…The subsequent booster doses of peptide emulsified in incomplete Freund's adjuvant were given at two-week intervals after the first injection. IgY was isolated with polyethylene glycol 6000 (PEG 6000; Sigma-Aldrich) using a precipitation method [40][41][42][43]. Briefly, after disinfection of the eggshells with 70% ethanol, the yolks were poured into a 50 mL Falcon™ tube and mixed with PBS.…”