Method for Primary Screening of Pharmaceuticals on the Paramecium caudatum Eukaryotic Cell Model

Gruzdev, G. A.; Карпухина, О.В.; Иноземцев, А. Н.; Kamensky, A.A.

doi:10.1134/s1607672922010033

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…This is primarily achieved via chemoreceptors and represents one particularly important behavior for aquatic species that significantly determine their chance of survival [ 48 , 49 ]. Similar to other aquatic species, Paramecium exhibited pronounced chemotaxis, and this is likely one of the behaviors they rely on to avoid hazards and pursue prey [ 50 ]. Numerous studies demonstrated the attraction and repulsion behavior of Paramecium in response to a set of defined chemical cues, strongly indicating the presence of specific chemoreceptors [ 25 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Paramecium bursaria as a Potential Tool for Evaluation of Microplastics Toxicity

Zhang

Chen

et al. 2022

Biology

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Microplastics (MPs) are normally defined as small plastic wastes with a size of 1 μm to 5 mm in diameter. This tiny plastic debris is abundant in aquatic systems and poses a great threat to aquatic biota. To date, toxicological assessment of MPs is predominantly dependent on metazoan animals, although their applications are sometimes limited due to the high cost, narrow ecological niche, or ethical considerations. In this regard, unicellular eukaryotes (i.e., protozoa) that are ubiquitously present in nature represent a promising alternative for evaluating the toxicity of MPs. In this study, we selected Paramecium bursaria (P. bursaria) as a representative of protozoa and further investigated behavioral and molecular changes in MPs-exposed P. bursaria. Our results showed that following MPs uptake, P. bursaria exhibited various changes, including anomalies in swimming patterns, reduction in moving speed, impairment of avoidance behavior, elevation of oxidative stress, and potential disturbance of endosymbiosis. These elicited changes in P. bursaria in response to MPs exposure were pronounced and measurable. Overall, this study demonstrated that P. bursaria could serve as a promising alternative for the toxicological assessment of MPs and may be further applied to evaluate the toxicity of other environmental contaminants.

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Section: Discussionmentioning

confidence: 99%

Paramecium bursaria as a Potential Tool for Evaluation of Microplastics Toxicity

Zhang

Chen

et al. 2022

Biology

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The Effect of the Agonist Cholecystokinin-4 D-GB-115 On the Character of Motor Activity of Paramecium caudatum

Gruzdev

Soboleva

Kamensky

2023

Dokl Biochem Biophys

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THE EFFECT OF THE AGONIST CHOLECYSTOKININ-4 D-GB-115 ON THE CHARACTER OF MOTOR ACTIVITY OF <i>PARAMECIUM CAUDATUM</i>

Gruzdev,

Soboleva,

Kamensky

2023

Doklady Rossijskoj akademii nauk. Nauki o žizni

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The study investigated the effect of GABA in various concentrations and D-GB-115 at a concentration of 10–10 mol/ml on the behavior of Paramecium caudatum. It has been shown that GABA increases motor activity and changes the movement strategy of these protozoa, and the dose-effect relationship is domed, which can be explained by the presence of two types of GABA receptors in the outer membrane of paramecia: GABA-A and GABA-B. The range of active concentrations of GABA ranges from 10–6 to 10–16 mol/ml. The effect of pharmacological agents interacting with the GABA system on the behavior of infusoria: nembutal and D-GB-115.

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Method for Primary Screening of Pharmaceuticals on the Paramecium caudatum Eukaryotic Cell Model

Cited by 3 publications

References 7 publications

Paramecium bursaria as a Potential Tool for Evaluation of Microplastics Toxicity

Paramecium bursaria as a Potential Tool for Evaluation of Microplastics Toxicity

The Effect of the Agonist Cholecystokinin-4 D-GB-115 On the Character of Motor Activity of Paramecium caudatum

THE EFFECT OF THE AGONIST CHOLECYSTOKININ-4 D-GB-115 ON THE CHARACTER OF MOTOR ACTIVITY OF <i>PARAMECIUM CAUDATUM</i>

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