Method for the Determination of Hexosamines in Tissues

Boas, Norman F.

doi:10.1016/s0021-9258(18)66055-7

Cited by 1,447 publications

(36 citation statements)

References 7 publications

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“…The pooled hexane layers of each sample were dried under a stream of N2, esterified with 10% perchloric acid in methanol, and assayed for fatty acid methyl esters by gas-liquid chromatography. The aqueous phase was dried under N2 and applied to a column (1 by 8 cm) of Dowex-50-X8, H+ form in 2 ml of water (4). Two additional wash volumes of 2 ml each were added to the column, followed by 5 ml of water.…”

Section: Methodsmentioning

confidence: 99%

Distribution and composition of lipopolysaccharides from mycoplasmas

Smith¹,

Langworthy²,

Mayberry³

1976

J Bacteriol

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Polymeric carbohydrates containing glycerol and fatty acids were isolated from whole cells and membranes of mycoplasmas by hot aqueous phenol extraction and gel filtration. Lipopolysaccharides were found to occur in four species of Acholeplasma, two of Anaeroplasma, and in Mycoplasma neurolyticum. None were detected in Spiroplasma citri or in five species of Mycoplasma. All lipopolysaccharides contained both neutral and N-acylated amino sugars in ratios varying from 1:1 to 3:1. The neutral sugars found in varying distribution were glucose, galactose, and mannose. The amino sugars included fucosamine, an unidentified deoxyhexosamine, galactosamine, and glucosamine. Fucosamine and glucose were the only sugars common to all lipopolysaccharides. The fatty acids were similar to those found in the lipids of each organism.

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Section: Methodsmentioning

confidence: 99%

Distribution and composition of lipopolysaccharides from mycoplasmas

Smith¹,

Langworthy²,

Mayberry³

1976

J Bacteriol

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“…The following experiment (Table VIII) was performed to test the latter contingency. 3 Under the ordinary conditions of the assay for the inhibition of myofibrillar ATPase, we mixed myofibrils, microsomes, ATP and the customary reagents, and added a trace of Ca 45 to give sufficient counts. The total amount of Ca due to this addition and to uncontrolled impurities, as in the relaxation test, was not known, but may have been of the order of 10 -8 M. The ratio of microsomes to fibrils was high, enough to cause significant relaxation by top fraction II; the same excess was maintained for top fraction I, in order to illustrate the maximal extent of withdrawal of exchangeable calcium from the fibrils.…”

Section: Propertiesmentioning

confidence: 99%

Density Gradient Separation of Sarcotubular Vesicles and Other Particulate Constituents of Rabbit Muscle

Seraydarian

Mommaerts

1965

The Journal of Cell Biology

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Separation of particulate matter in rabbit muscle extracts by differential centrifugation leads, in first approximation, to the isolation of fraction I (15,000 to 41,000 g) and fraction II (41,000 to 150,000 g). The former consists mainly of sarcotubular material, actively transporting calcium ions, and displaying relaxation factor activity. The latter is heterogeneous, shows little calcium accumulation, and contains factors both inhibiting and activating myofibrillar ATPase. Fraction I is resolved by density gradient centrifugation into 2 main subfractions. The lighter one represents sarcotubular material in the best state of preservation, with biochemical activities stable for weeks in the cold. The heavier one may consist of the same material in a less well preserved form. Upon aging, it develops an activating activity toward myofibrillar ATPase, when the relaxing effect has declined. Fraction II is resolved by density gradient centrifugation into 3 or more fractions, with some variability. Relaxing activity in terms of inhibition of myofibrillar ATPase predominates among the lighter subfractions, increase of ATPase among the heavier. The intrinsic ATPase of fraction II is activated by calcium ions, but there is little or no bulk accumulation of calcium oxalate. Nevertheless, its limited calcium uptake seems to suffice to explain its relaxing activity. The particulate material contains mucopolysaccharide and lipid. Most of the lipid in fraction I is phospholipid; in fraction II this is less than half, if calculated as lecithin. The unfractionated material contains an adenylcyclase. There is no acetylcholine esterase.

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“…23) Chitin fraction thus obtained, was hydrolyzed using 6 N HCl autoclaved at 114°C for 6 h. N-acetylglucosamine levels were deter-mined according to the method of Boas. 24) All analyses were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics

Sato

Miyagi

Yoneyama

et al. 2017

Bioscience, Biotechnology, and Biochemistry

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Maitake mushroom (Grifola frondosa [Dicks.] Gray) is generally cultured using the sawdust of broadleaf trees. The maitake strain Gf433 has high production efficiency, with high-quality of fruiting bodies even when 30% of the birch sawdust on the basal substrate is replaced with conifer sawdust. We performed metabolome analysis to investigate the effect of different cultivation components on the metabolism of Gf433 and Mori52 by performing CE-MS on their fruiting bodies in different cultivation conditions to quantify the levels of amino acids, organic acids, and phosphorylated organic acids. We found that amino acid and organic acid content in Gf433 were not affected by the kind of sawdust. However, Gf433 contained more organic acids and less amino acids than Mori52, and Gf433 also contained more chitin compared with Mori52. We believe that these differences in the metabolome contents of the two strains are related to the high production efficiency of Gf433.

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Method for the Determination of Hexosamines in Tissues

Cited by 1,447 publications

References 7 publications

Distribution and composition of lipopolysaccharides from mycoplasmas

Distribution and composition of lipopolysaccharides from mycoplasmas

Density Gradient Separation of Sarcotubular Vesicles and Other Particulate Constituents of Rabbit Muscle

CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics

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