Method for the Rapid Detection of SARS-CoV-2-Neutralizing Antibodies Using a Nanogel-Based Surface Plasmon Resonance Biosensor

Lee, Su-Kyoung; Yim, Bora; Park, Jinseul; Kim, Nam-Gun; Kim, Byung Soo; Park, Yongdoo; Yoon, Young Kyung; Kim, Jong‐Seong

doi:10.1021/acsapm.2c02187

Cited by 8 publications

(10 citation statements)

References 24 publications

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“…In this study, we developed analytical modalities for α-thrombin detection based on its specific binding to the platelet protein GPIbα and tested different methods to investigate in-depth detection protocols via MPB and to demonstrate easy and timely detection of this candidate marker. Our nanogel platform, known for its proficiency in ligand detection such as IL-2 and SARS-COV-2 in SPR analysis, , was used by functionalizing it with GPIbα. The nSPR assay indicated that GPIbα was suitable for α-thrombin detection with not only high-fidelity binding but also wide dynamic ranges.…”

Section: Discussionmentioning

confidence: 99%

“…However, microscopic imaging lacks sensitivity for the accurate quantification of target molecules and requires sophisticated instrumentation. To overcome these limitations, bioresponsive nanogels have been employed as sensing platforms in surface plasmon resonance (SPR) analysis to investigate the MPB of programmed cell death protein 1, interleukin-2 (IL-2) receptor α, and the receptor-binding domain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to their ligands. − …”

Section: Introductionmentioning

confidence: 99%

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Detection of α-Thrombin with Platelet Glycoprotein Ibα (GP1bα) for the Development of a Coagulation Marker

Oh,

Chae,

Teoh

et al. 2024

ACS Omega

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The detection of prothrombotic markers is crucial for understanding thromboembolism and assessing the effectiveness of anticoagulant drugs. α-Thrombin is a marker that plays a critical role in the coagulation cascade process. However, the detection of this enzymatic molecule was hindered by the absence of an efficient modality in the clinical environment. Previously, we reported that one α-thrombin interacts with two α-chains of glycoprotein Ib (GPIbα), i.e., multivalent protein binding (MPB), using bioresponsive hydrogel nanoparticles (nanogels) and optical microscopy. In this study, we demonstrated that GPIbα-mediated platforms led to the highly sensitive and quantitative detection of α-thrombin in various diagnostic systems. Initially, a bioresponsive nanogel-based surface plasmon resonance (nSPR) assay was developed that responds to the MPB of α-thrombin to GPIbα. The use of GPIbα for the detection of α-thrombin was further validated using the enzyme-linked immunosorbent assay, which is a goldstandard protein detection technique. Additionally, GPIbα-functionalized latex beads were developed to perform latex agglutination (LA) assays, which are widely used with hospital diagnostic instruments. Notably, the nSPR and LA assays exhibited a nearly 1000fold improvement in sensitivity for α-thrombin detection compared to our previous optical microscopy method. The superiority of our GPIbα-mediated platforms lies in their stability for α-thrombin detection through protein−protein interactions. By contrast, assays relying on α-thrombin enzymatic activity using substrates face the challenge of a rapid decrease in postsample collection. These results suggested that the MPB of α-thrombin to GPIbα is an ideal mode for clinical α-thrombin detection, particularly in outpatient settings.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of α-Thrombin with Platelet Glycoprotein Ibα (GP1bα) for the Development of a Coagulation Marker

Oh,

Chae,

Teoh

et al. 2024

ACS Omega

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“…Deformable nanogels were prepared using aqueous free-radical precipitation polymerization as previously described 46, 47 . Briefly, an 88% pNIPAM/10% AAc/2% BIS solution (poly( N -isopropylacrylamide- co -acrylic acid)) was used for nanogel synthesis.…”

Section: Methodsmentioning

confidence: 99%

Antibody-conjugating nanogel (Conjugel) with two immune checkpoint inhibitors for enhanced cancer immunotherapy

Chae,

Lee,

et al. 2023

Preprint

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Cancer immunotherapy by immune checkpoint inhibitors (ICI) acts on antitumor responses by stimulating the immune system to attack cancer cells. However, this powerful therapy is hampered by its high treatment cost and limited efficacy. Here, we show the development of an antibody-conjugating system (Conjugel) that potentiates the efficacy of bispecific immunotherapy that simultaneously targets CTLA-4 and PD-L1. The Conjugel, consisting of highly deformable nanogels and antibody-binding protein, was loaded with two ICI monoclonal antibodies (mAb). Compared with mAb treatment alone, treatment with a bispecific Conjugel loaded with the both ICIs significantly decreased both the survival of MCF-7 and MDA-MB-231 breast cancer cells in vitro and the size of 4T1-Luc2-derived orthotopic syngeneic tumors in vivo. Furthermore, the ICI-loaded Conjugel was less toxic in vivo than the combination treatment delivered as a bolus. Our findings have important implications for Conjugel-based immunotherapy, developing the safer and higher efficacy of ICIs to treat breast cancers.

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“…63 Functionalized NGs with the receptor-binding domain of the SARS-CoV-2 spike protein have served to detect neutralizing antibodies in the sera of individuals vaccinated against COVID-19. 66 Furthermore, hybrid NGs 67 based on Nisopropylacrylamide/N,N′-methylenebis(acrylamide) (NI-PAAM/MBA) and Cr 2 O 3 nanosheets and further decorated with AuNPs have been used to detect H 2 O 2 by surfaceenhanced Raman spectroscopy (SERS) with LOD = 10 −8 mol/ L. Also, thermoresponsive lysozyme/calcium alginate-g-poly-(NIPAAM) nanohydrogels 68 (Lys/CA-g-PNIPAAm NHs) were used to obtain a lysozyme molecularly imprinted sensor, which can selectively rebind to lysozyme in a mixture of closely related compounds. This sensor exhibited a wide linear range from 1 × 10 −9 to 1 × 10 −3 mg/mL at 30 °C.…”

Section: Hyperbranched Polymers (Hbps)mentioning

confidence: 99%

“…Functionalized NGs with the receptor-binding domain of the SARS-CoV-2 spike protein have served to detect neutralizing antibodies in the sera of individuals vaccinated against COVID-19 . Furthermore, hybrid NGs based on N -isopropylacrylamide/ N , N ′-methylenebis(acrylamide) (NIPAAM/MBA) and Cr 2 O 3 nanosheets and further decorated with AuNPs have been used to detect H 2 O 2 by surface-enhanced Raman spectroscopy (SERS) with LOD = 10 –8 mol/L.…”

Section: Organic Nanomaterials For Healthcare Biosensingmentioning

confidence: 99%

Review on Healthcare Biosensing Nanomaterials

Tripathi

Bonilla‐Cruz

2023

ACS Appl. Nano Mater.

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Fast technological progress has sped up the growth of telemedicine, mobile e-health services, and healthcare monitoring, which, nowadays, are technologies in high demand in the medical field and analytical sensing. Biosensing device design has promoted forefront research topics wherein new sensing technologies have been developed. In this regard, nanomaterials have had a crucial role wherein novel biosensors based on nanomaterials have emerged as detective tools for several biomolecules. Most biosensors are remarkably sensitive and selective to detect cancer biomarkers, toxins in foods, drugs, pathogenic microorganisms, cholesterol, pesticides, nucleic acids, glucose, heavy-metal contaminations, and other bioanalytes under different platforms at extraordinarily low concentrations (nanomolar, picomolar, or femtomolar). A comprehensive review of nanomaterials used for healthcare biosensing is offered herein. Specifically, this review offers a new point of view, highlighting and covering exhaustively several kinds of nanomaterials (<500 nm) to detect human diseases through biosensing. In this sense, we organize them into organic, inorganic, and carbon-based, thus offering a guide to selecting the best nanomaterial for specific detection. Further, it provides a roadmap to the real-time utilization of nanomaterials at a commercial scale. Also, this review addresses some crucial aspects of nanomaterials use and applications for healthcare biosensing. The progress discussed in this paper highlights the immense potential to implement the use of nanomaterials and nanocomposites in the initial examination of diseases and point-of-care testing.

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Method for the Rapid Detection of SARS-CoV-2-Neutralizing Antibodies Using a Nanogel-Based Surface Plasmon Resonance Biosensor

Cited by 8 publications

References 24 publications

Detection of α-Thrombin with Platelet Glycoprotein Ibα (GP1bα) for the Development of a Coagulation Marker

Detection of α-Thrombin with Platelet Glycoprotein Ibα (GP1bα) for the Development of a Coagulation Marker

Antibody-conjugating nanogel (Conjugel) with two immune checkpoint inhibitors for enhanced cancer immunotherapy

Review on Healthcare Biosensing Nanomaterials

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