Méthodes d’évaluation de la microarchitecture de l’os trabéculaire humain

Chappard, Christine

doi:10.1051/medsci/20122812022

Cited by 6 publications

(2 citation statements)

References 37 publications

(38 reference statements)

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“…Indeed, the lesion is completely filled 150 days after the surgery; however, additional biomechanical tests should be conducted to ensure that the newly formed tissue is as hard and resistant as the original bone. Bone mineral density could be measured by two-photon X-ray absorptiometry (bone densitometry), for example [ 47 ]. In addition, Raman spectroscopy could provide information on the level of hydroxyapatite and collagen in bone tissue [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

et al. 2020

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Oral diseases have an impact on the general condition and quality of life of patients. After a dento-alveolar trauma, a tooth extraction, or, in the case of some genetic skeletal diseases, a maxillary bone defect, can be observed, leading to the impossibility of placing a dental implant for the restoration of masticatory function. Recently, bone neoformation was demonstrated after in vivo implantation of polycaprolactone (PCL) biomembranes functionalized with bone morphogenic protein 2 (BMP-2) and ibuprofen in a mouse maxillary bone lesion. In the present study, human bone marrow derived mesenchymal stem cells (hBM-MSCs) were added on BMP-2 functionalized PCL biomembranes and implanted in a maxillary bone lesion. Viability of hBM-MSCs on the biomembranes has been observed using the “LIVE/DEAD” viability test and scanning electron microscopy (SEM). Maxillary bone regeneration was observed for periods ranging from 90 to 150 days after implantation. Various imaging methods (histology, micro-CT) have demonstrated bone remodeling and filling of the lesion by neoformed bone tissue. The presence of mesenchymal stem cells and BMP-2 allows the acceleration of the bone remodeling process. These results are encouraging for the effectiveness and the clinical use of this new technology combining growth factors and mesenchymal stem cells derived from bone marrow in a bioresorbable membrane.

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Section: Discussionmentioning

confidence: 99%

A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

et al. 2020

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“…La microtomographie (micro-computed-tomography, (µ-CT)) est une méthode permettant la visualisation et la quantification des paramètres de l'os trabéculaire et cortical. [14] Cette analyse 3D est complémentaire de l'analyse histologique en 2D.…”

Section: C) Analyse De La Microarchitecture Osseuse Par 3d-microctunclassified

La réponse inadéquate aux traitements anti-ostéoporotiques est liée à la persistance de la résorption osseuse chez les patients ostéoporotiques

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Cormier

et al. 2021

Revue du Rhumatisme

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Technical note: early post-mortem changes of human bone in taphonomy with μCT

et al. 2016

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Post-mortem interval (PMI) estimation is an important issue in forensic medicine, particularly for criminal purposes and legal limitation periods. The goal of the present study is to examine the evolution of the trabecular cranial vault bone after 4 weeks of conservation in a controlled environment with micro-tomography (μCT) analyses.Four bone samples were extracted from a fresh human cranial vault (a donation to science according to the French law) and conserved in an air-controlled environment. The samples were weighed and μCT scanned at a 10-μm resolution every week after death for a month. The μCT features were identical for every sample. Each set of data from the μCTs was reconstructed, registered, and analyzed in terms of the total volume, bone volume, bone surface, number of trabeculae, trabeculae thickness, and mean distance of the trabeculae. The samples were conserved in a glass box in 20 °C air with 60% humidity in a laboratory hood between each μCT acquisition. Descriptive statistics were determined. Each sample was observed and compared to itself over time.After 1 month of conservation, the mean bone volume (-1.9%), bone surface (-5.1%), and trabecular number (-12.35%) decreased, whereas the mean trabecular separation (+5.55%) and trabecular thickness (+12.7%) increased. Many variations (i.e., increases and decreases) were observed between the extraction of the sample and the end of the 4 weeks of conservation. The present observations may be explained by bone diagenesis. Previous observations have indicated that protein and lipid losses occur with bone weight and volume losses. These diagenesis effects may explain the trabecular modifications observed in the present work. We observed many bone variations with the μCT scans between the beginning and the end of the conservation that had no explanations. Additional studies, particularly studies involving statistics, need to be performed to confirm our observations and explain these results more clearly.

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Méthodes d’évaluation de la microarchitecture de l’os trabéculaire humain

Cited by 6 publications

References 37 publications

A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

A New Polycaprolactone-Based Biomembrane Functionalized with BMP-2 and Stem Cells Improves Maxillary Bone Regeneration

La réponse inadéquate aux traitements anti-ostéoporotiques est liée à la persistance de la résorption osseuse chez les patients ostéoporotiques

Technical note: early post-mortem changes of human bone in taphonomy with μCT

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