Méthodes d’évaluation du potentiel antioxydant dans les aliments

Marc, Françoise; Davin, André; Deglène-Benbrahim, Laurence; Ferrand, Carine; Baccaunaud, Michel; Fritsch, P.

doi:10.1051/medsci/2004204458

Cited by 31 publications

(21 citation statements)

References 34 publications

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“…Recent studies reported the effect of fermentation on antioxidant properties in some food products. Young‐Hee et al 34 observed an increase in DPPH radical scavenging in soybeans fermented by lactic acid bacteria, essentially due to the presence of compounds such as mineral salts and phenols 35. Depending on the strain used, bacterial metabolism might influence the total antioxidant activity in carrot juice.…”

Section: Resultsmentioning

confidence: 99%

Synbiotic potential of carrot juice supplemented with Lactobacillus spp. and inulin or fructooligosaccharides

et al. 2008

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Section: Resultsmentioning

confidence: 99%

Synbiotic potential of carrot juice supplemented with Lactobacillus spp. and inulin or fructooligosaccharides

et al. 2008

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“…The assay was performed following the method described by Marc et al (2016) which was adapted to microplates. Briefly, an aqueous ABTS + solution was prepared by the oxidation reaction of ABTS (Sigma Aldrich, Missouri, USA) with MnO 2 .…”

Section: Total Hydrosoluble Antioxidant Assaymentioning

confidence: 99%

Differences in Blossom-end Rot Resistance in Tomato Cultivars is Associated with Total Ascorbate rather than Calcium Concentration in the Distal End Part of Fruits <i>per se</i>

et al. 2018

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Calcium is widely accepted as the main factor responsible for blossom-end rot (BER) appearance in tomato (Solanum lycopersicum L.) fruit. However, reactive oxygen species (ROS), which can damage plant tissues have also been proposed to initiate BER appearance in tomatoes and other fruit-bearing vegetables. Ascorbate, the major antioxidant in tomato fruit, is generally lower during green fruit development, which corresponds to the stage of BER appearance. Accordingly, one hypothesis is that tomato cultivars with a lower susceptibility to BER under salt stress have higher ascorbate contents and thus better control of ROS levels. In this study, to clarify the relationship between BER incidence and oxidative stress, two BER resistant cultivars, 'Managua RZ' and 'House Momotaro' and one BER-susceptible cultivar 'Reiyoh', were cultivated under salinity or standard nutrient solution (control) conditions. Calcium, potassium, magnesium, total hydro-soluble antioxidants, and ascorbate concentrations were measured in the distal pericarp 1 to 2 days prior to symptom appearance and during symptom appearance in healthy and affected fruits. When salt stress was applied, only BER-resistant cultivars showed a significant increase in ascorbate contents prior to BER appearance as compared with their levels under the control condition. In contrast, pre-BER Ca concentrations. A similar tendency was also observed in apple "bitter pit". The ability to increase the fruit antioxidant capacity and maintain mineral balance under salt stress conditions may explain the resistance to BER development in highly resistant cultivars, probably by the avoidance of oxidative-induced cell necrosis and stabilization of the cell membranes, respectively.

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“…32 The ABTS + with the peak absorbance at 743 nm delivers a bluish-green color which is raised by the loss of an electron by the nitrogen atom of ABTS (2,2-azino-bis3ethylbenzthiazoline-6-sulphonic acid). 33 ABTS can be oxidized by potassium persulphate 34 generating ABTS cation radicals (ABTS •+ ). Based on the results of the present study, AgNPs demonstrated high antioxidant activities.…”

Section: Discussionmentioning

confidence: 99%

The Antioxidant Activity and Cytotoxic Effects of Amaranthus cruentus-Biosynthesized Silver Nanoparticles Toward MCF-7 Breast Cancer Cell Line

Baghani

Es‐haghi

2019

Int J Basic Sci Med

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Introduction: Silver nanoparticles (AgNPs) have grabbed special attention owing to their exclusive structural features. Green synthesis (i.e., plant-mediated) of AgNPs is an efficient and cost-effective method with widespread clinical applications. Therefore, the present study aimed to synthesize AgNPs based on green synthesis method employing the seed extracts of Amaranthus cruentus and to investigate the antioxidant and cytotoxic activities of the biosynthesized AgNPs. Methods: The Ag-NPs were biologically synthesized using the A. cruentus extract which served as a reducing agent. Then, the synthesized Ag-NPs were visualized by transmission electron microscopy. Next, the antioxidant activity of the synthesized Ag-NPs was evaluated by DPPH and ABTS methods. Finally, the cytotoxicity of AgNPs was investigated against MCF-7 breast cancer cell line using MTT assay. Results: The mean diameter of the synthesized Ag-NPs ranged from 20 to 40 nm. In addition, the IC50 of free radical scavenging activity of the Ag-NPs were obtained as 500 µg/mL (DPPH) and 400 µg/mL (ABTS). Further, the AgNPs showed time and dose-dependent cytotoxicity against MCF-7 cells. Eventually, at the 24-hour exposition to the 80 µg/mL dose of AgNPs, the viability of cancerous cells was 19% plunging to 2.03% and 1.9% after 48 hours and 72 hours, respectively. Conclusion: In general, plant extracts can serve as facile and eco-friendly alternatives to hazardous methods for synthesizing the metal nanoparticles. Therefore, the A. cruentus biosynthesized AgNPs can be utilized in medicine for various purposes due to their low toxicity and appropriate antioxidant activity.

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Méthodes d’évaluation du potentiel antioxydant dans les aliments

Cited by 31 publications

References 34 publications

Synbiotic potential of carrot juice supplemented with Lactobacillus spp. and inulin or fructooligosaccharides

Synbiotic potential of carrot juice supplemented with Lactobacillus spp. and inulin or fructooligosaccharides

Differences in Blossom-end Rot Resistance in Tomato Cultivars is Associated with Total Ascorbate rather than Calcium Concentration in the Distal End Part of Fruits <i>per se</i>

The Antioxidant Activity and Cytotoxic Effects of Amaranthus cruentus-Biosynthesized Silver Nanoparticles Toward MCF-7 Breast Cancer Cell Line

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