Methodological Aspects of REM Sleep- Deprivation and Stereological Protocols in the Brain-Stem Respiratory Nuclei

Kamali, Ali Mohammad; Noorafshan, Ali; Karimi, Fatemeh; Karbalay‐Doust, Saied

doi:10.18869/nrip.jamsat.2.3.283

Cited by 9 publications

(11 citation statements)

References 16 publications

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“…The method of limiting sleep in this study was rapid eye movement (REM) sleep deprivation. When the animals enter the REM sleep, they fell into the water due to muscle relaxation and consequently woke up [33].…”

Section: Induction Of Chronic Sleep Deprivationmentioning

confidence: 99%

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Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

et al. 2020

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Background: In modern societies, sleep deprivation is a serious health problem. This problem could be induced by a variety of reasons, including lifestyle habits or neurological disorders. Chronic sleep deprivation (CSD) could have complex biological consequences, such as changes in neural autonomic control, increased oxidative stress, and inflammatory responses. The superior cervical ganglion (SCG) is an important sympathetic component of the autonomic nervous system. CSD can lead to a wide range of neurological consequences in SCG, which mainly supply innervations to circadian system and other structures. As the active component of Curcuma longa, curcumin possesses many therapeutic properties; including neuroprotective. This study aimed to evaluate the effect of CSD on the SCG histomorphometrical changes and the protective effect of curcumin in preventing these changes. Methods: Thirty-six male rats were randomly assigned to the control, curcumin, CSD, CSD + curcumin, grid floor control, and grid floor + curcumin groups. The CSD was induced by a modified multiple platform apparatus for 21 days and animals were sacrificed at the end of CSD or treatment, and their SCGs removed for stereological and TUNEL evaluations and also spatial arrangement of neurons in this structure. Results: Concerning stereological findings, CSD significantly reduced the volume of SCG and its total number of neurons and satellite glial cells in comparison with the control animals (P < 0.05). Treatment of CSD with curcumin prevented these decreases. Furthermore, TUNEL evaluation showed significant apoptosis in the SCG cells in the CSD group, and treatment with curcumin significantly decreased this apoptosis (P < 0.01). This decrease in apoptosis was observed in all control groups that received curcumin. CSD also changed the spatial arrangement of ganglionic neurons into a random pattern, whereas treatment with curcumin preserved its regular pattern. Conclusions: CSD could potentially induce neuronal loss and structural changes including random spatial distribution in the SCG neurons. Deleterious effects of sleep deprivation could be prevented by the oral administration of curcumin. Furthermore, the consumption of curcumin in a healthy person might lead to a reduction of cell death.

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Section: Induction Of Chronic Sleep Deprivationmentioning

confidence: 99%

“…The grid floor prevented rats to fall into the water and so, they could sleep. This induced stress without sleep deprivation and can be considered as the control for the CSD group [33]. The rats in the control group were routinely fed and gavaged with PBS for 21 days and kept in their cages in the same room.…”

Section: Induction Of Chronic Sleep Deprivationmentioning

confidence: 99%

Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

et al. 2020

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“…The experimental groups comprised Group I (control) which remained in their standard home cage, Group II (grid-floor control) which were placed in cages with a grid-floor, and Group III which underwent CSR using the modified multiple-platform method. After a period of 21 days of the CSR procedure (18-h sleep deprivation per day for three consecutive weeks) [19], animals were submitted to the rotarod test and were subsequently killed to have their brains excised and processed possibly for future histomorphological investigations.…”

Section: Animalsmentioning

confidence: 99%

“…The setup used in this study was a modified multi-platform (mMP) container made of plastic that have been explained earlier [19,20]. The box floor contained 15 cylindrical platforms, 8.0 cm high and 6.5 cm in diameter.…”

Section: Chronic Sleep Restrictionmentioning

confidence: 99%

“…First, the rats were tested at a fixed-speed rotarod procedure and then on the accelerating rotarod procedure for 2 days. During the testing period, animals were tested at 8 different speeds, including 12,16,19,21,24,26,28, and 38 rpm, for the maximum of 60 s at each speed. Rats experienced the test three times at each speed with an interval of 20 min between each test.…”

Section: Motor Performance Testmentioning

confidence: 99%

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Motor Control in Chronically Sleep-Restricted Rats: A Behavioral Approach

et al. 2018

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Objective To consider the effect of chronic sleep restriction (CSR) on motor impairment in sleep-restricted rats. Methods A total of 21 adult male Sprague-Dawley rats were assigned to three groups including control, grid-floor control and CSR using the modified multiple-platform method. At the end of experiment, motor performance was assessed by means of the rotarod test. Results Performance of the CSR rats in the fixed and accelerating speed rotarod tasks was impaired and their endurance time was less than the control group (p < 0.01). Conclusion The rotarod motor performance of the CSR rats was reduced, suggesting an impaired motor control function following sleep restriction.

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Restorative effects of curcumin on sleep-deprivation induced memory impairments and structural changes of the hippocampus in a rat model

et al. 2017

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Methodological Aspects of REM Sleep- Deprivation and Stereological Protocols in the Brain-Stem Respiratory Nuclei

Cited by 9 publications

References 16 publications

Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

Motor Control in Chronically Sleep-Restricted Rats: A Behavioral Approach

Restorative effects of curcumin on sleep-deprivation induced memory impairments and structural changes of the hippocampus in a rat model

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