Methodologies for Microscopic Characterization of Ocular Toxicity

Teixeira, Leandro; Render, James A.

doi:10.1007/7653_2013_5

Cited by 2 publications

(1 citation statement)

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“…Main challenges associated with ocular devices relate to the difficulties in producing quality histology preparations with this tissue, namely, for adequate fixation and careful handling and trimming and have been described elsewhere (Teixeira and Render n.d.) and within this issue (Drevon-Gaillot 2019).…”

Section: Skeletal System (Bones Teeth Ligaments)mentioning

confidence: 99%

Histology Strategies for Medical Implants and Interventional Device Studies

Rousselle¹,

Wicks²,

Tabb³

et al. 2019

Toxicol Pathol

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Histology of medical devices poses a variety of unique challenges. Comprehensive histologic assessment of medical devices often requires spatial context and high-quality retention of the device–tissue interface. However, the composition of many medical devices is often not amenable to traditional paraffin embedding and thus alternative specialized methodologies such as hard resin embedding must be used. Hard resin embedding requires specialized laboratory technical expertise and equipment, and the fixation techniques and resin composition used markedly impact the feasibility of immunohistochemistry. For the continuity of spatial context during histologic evaluation, additional imaging methods such as macrophotography, radiography, micro-Computerized Tomography (microCT), or magnetic resonance imaging (MRI) can be used to guide sectioning and to complement histologic findings. Although standardized approaches are scarce for medical devices, important considerations specific to medical device histology are discussed, including general specimen preparation, special considerations for devices by organ system, and the challenges of immunohistochemistry. Histologic preparation of medical devices must be thoughtful, thorough, and tailored to achieve optimal histologic outcomes for complex, valuable, and often limited implant specimens.

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Section: Skeletal System (Bones Teeth Ligaments)mentioning

confidence: 99%