Methodology An alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry (Rubus L.)

Chen, Q; Yu, Haowei; Wang, X R; Xie, Xiao-Xun; Yue, Xueping; Tang, Haoru

doi:10.4238/2012.june.29.10

Cited by 53 publications

(36 citation statements)

References 22 publications

(32 reference statements)

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“…Total RNA was extracted from samples using the improved CTAB method . After quality assessment, 1 μg of total RNA was reverse‐transcribed into the complementary DNA (cDNA) with PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Japan) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Identification of NADPH oxidase family members associated with cold stress in strawberry

Zhang

et al. 2018

FEBS Open Bio

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NADPH oxidase is encoded by a small gene family (Respiratory burst oxidase homologs, Rbohs) and plays an important role in regulating various biological processes. However, little information about this gene family is currently available for strawberry. In this study, a total of seven Rboh genes were identified from strawberry through genomewide analysis. Gene structure analysis showed the number of exons ranged from 10 to 23, implying that this variation occurred in FvRboh genes by the insertion and distribution of introns; the order and approximate size of exons were relatively conserved. FvRbohC was predicted to localize to the thylakoid membrane of the chloroplast, while other members were computed to localize to the plasma membrane, indicating different functions. Amino acid sequence alignment, conserved domain, and motif analysis showed that all identified FvRbohs had typical features of plant Rbohs. Phylogenetic analysis of Rbohs from strawberry, grape, Arabidopsis, and rice suggested that the FvRbohs could be divided into five subgroups and showed a closer relationship with those from grape and Arabidopsis than those from rice. The expression patterns of FvRboh genes in root, stem, leaf, flower, and fruit revealed robust tissue specificity. The expression levels of FvRbohA and FvRbohD were quickly induced by cold stress, followed by an increase in NADPH oxidase activity, leading to O2− accumulation and triggering the antioxidant reaction by the transient increases in SOD activity. This suggested these two genes may be involved in cold stress and defense responses in strawberry.

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Section: Methodsmentioning

confidence: 99%

Identification of NADPH oxidase family members associated with cold stress in strawberry

Zhang

et al. 2018

FEBS Open Bio

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“…Kassim et al [16] mapped QTLs for individual anthocyanin pigments in raspberry. The genes of various enzymes of flavonoid biosynthesis were also identified in red [18] and black [25] raspberry and blackberry [26]. Besides the structural genes, regulatory genes are important in the biosynthesis of flavonoids.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity in Differently Colored Raspberry Cultivars Using SSR Markers Located in Flavonoid Biosynthesis Genes

et al. 2019

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Raspberry is a valuable berry crop containing a large amount of antioxidants that correlates with the color of the berries. We evaluated the genetic diversity of differently colored raspberry cultivars by the microsatellite markers developed using the flavonoid biosynthesis structural and regulatory genes. Among nine tested markers, seven were polymorphic. In total, 26 alleles were found at seven loci in 19 red (Rubus idaeus L.) and two black (R. occidentalis L.) raspberry cultivars. The most polymorphic marker was RiMY01 located in the MYB10 transcription factor intron region. Its polymorphic information content (PIC) equalled 0.82. The RiG001 marker that previously failed to amplify in blackberry also failed in black raspberry. The raspberry cultivar clustering in the UPGMA dendrogram was unrelated to geographical and genetic origin, but significantly correlated with the color of berries. The black raspberry cultivars had a higher homozygosity and clustered separately from other cultivars, while at the same time they differed from each other. In addition, some of the raspberry cultivars with a yellow-orange color of berries formed a separate cluster. This suggests that there may be not a single genetic mechanism for the formation of yellow-orange berries. The data obtained can be used prospectively in future breeding programs to improve the nutritional qualities of raspberry fruits.

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“…Total RNA was extracted by modified CTAB method [9]. cDNA was synthesized according to the instructions of the Reverse Script kit Prime Script RT reagent Kit with gDNA Eraser (Takara, Dalian, China).…”

Section: Total Rna Extraction and Reverse Transcriptionmentioning

confidence: 99%

Cloning and Expression Analysis of CER1 in Blackberry (Rubus Spp.)

Liu¹,

Wang²,

Tang³

et al. 2018

Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

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Abstract.To understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene CER1 was cloned from blackberry. The open reading frame of this gene was 1878 bp, encoding 625 amino acid residues with a molecular weight of 72.3 kD and a theoretical isoelectric point of 8.59. Its amino acid sequence has the highest identity with strawberry. The results of real-time quantitative PCR showed that the gene expression increased with the maturation of blackberry. With the increase of the ratio of red or blue light, the expression of CER1 gene firstly decreased and then increased. Under the condition of red and blue light at a ratio of 2:8, the expression of CER1 was the lowest. The reason may be related to the different mechanisms of photoreceptor response to red and blue light.

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Methodology An alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry (Rubus L.)

Cited by 53 publications

References 22 publications

Identification of NADPH oxidase family members associated with cold stress in strawberry

Identification of NADPH oxidase family members associated with cold stress in strawberry

Assessment of Genetic Diversity in Differently Colored Raspberry Cultivars Using SSR Markers Located in Flavonoid Biosynthesis Genes

Cloning and Expression Analysis of CER1 in Blackberry (Rubus Spp.)

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