Methods being developed for preparation, delivery and transplantation of a tissue-engineered corneal endothelium

Proulx, Stéphanie; Brunette, Isabelle

doi:10.1016/j.exer.2011.06.013

Cited by 50 publications

(35 citation statements)

References 94 publications

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“…These procedures opened the possibility to replace the corneal endothelium with endothelium reconstructed by bioengineering. 67 However, clinically available procedures with artificial corneas have limitations such as inflammation of the retroprosthetic membrane and development of glaucoma, and are reserved for high-risk patients. 68,69 The limited availability of donor corneas and the current issues in surgical procedures require the development of new methods in the field of tissue engineering in order to improve corneal endothelial cell survival and increase corneal endothelial cell density.…”

Section: Clinical Conditions Of Corneal Endothelium and Current Treatmentioning

confidence: 99%

Corneal endothelium: developmental strategies for regeneration

Zavala

Jaime

Barrientos

et al. 2013

Eye

113

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The main treatment available for restoration of the corneal endothelium is keratoplasty. This procedure is faced with several difficulties, including the shortage of donor tissue, postsurgical complications associated with the use of drugs to prevent immune rejection, and a significant increase in the occurrence of glaucoma. Recently, surgical procedures such as Descemet's stripping endothelial keratoplasty have focused on the transplant of corneal endothelium, yielding better visual results but still facing the need for donor tissue. The emergent strategies in the field of cell biology and tissue cultivation of corneal endothelial cells aim at the production of transplantable endothelial cell sheets. Cell therapy focuses on the culture of corneal endothelial cells retrieved from the donor, in the donor's cornea, followed by transplantation into the recipient. Recently, research has focused on overcoming the challenge of harvesting human corneal endothelial cells and the generation of new biomembranes to be used as cell scaffolds in surgical procedures. The use of corneal endothelial precursors from the peripheral cornea has also demonstrated to be effective and represents a valuable tool for reducing the risk of rejection in allogeneic transplants. Several animal model reports also support the use of adult stem cells as therapy for corneal diseases. Current results represent important progresses in the development of new strategies based on alternative sources of tissue for the treatment of corneal endotheliopathies. Different databases were used to search literature: PubMed, Google Books, MD Consult, Google Scholar, Gene Cards, and NCBI Books. The main search terms used were: 'cornea AND embryology AND transcription factors', 'human endothelial keratoplasty AND risk factors', '(cornea OR corneal) AND (endothelium OR endothelial) AND cell culture', 'mesenchymal stem cells AND cell therapy', 'mesenchymal stem cells AND cornea', and 'stem cells AND (cornea OR corneal) AND (endothelial OR endothelium)'.

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Section: Clinical Conditions Of Corneal Endothelium and Current Treatmentioning

confidence: 99%

Corneal endothelium: developmental strategies for regeneration

Zavala

Jaime

Barrientos

et al. 2013

Eye

113

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“…The ocular bulbs and cornea sizes of pigs, their morphological endothelium characteristics, their wide availability and their low sale prices are some of the biggest advantages of this choice in medicine and veterinary medicine (Proulx & Brunette 2012). Due to all of these reasons, the ocular models for this research were swine ocular bulbs.…”

Section: Discussionmentioning

confidence: 99%

Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

et al. 2016

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ABSTRACT.-Terzariol M., Hünning P.S., Brambatti G., Albuquerque L., Neumann C. & Pigatto J.A. T. 2016 The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue ® ) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.INDEX TERMS: Eye, brilliant blue 0.05%, toxicity, corneal endothelium, swine.

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“…Since Stocker et al (1) established a human corneal endothelial cell (HCEC) culture, the potential for cell therapy to treat corneal endothelial dysfunction using HCECs has demonstrated continuous development (2)(3)(4)(5)(6)(7)(8) . The limitations associated with this therapy can be basically divided into two major areas: those related to culturing the cells, such as proliferation, cellular senescence, and fibroblastic transformation and those related to the logistics and techniques for transplanting the cells (2)(3)(4)(5)(6)(7)(8) .…”

Section: Introductionmentioning

confidence: 99%