2016
DOI: 10.1556/1326.2016.28.4.3
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Methods for analysis of thiamin and folic acid by HPLC—DAD in fortified rice pure and mixed to milled rice before and after different cooking techniques

Abstract: Summary. This study aimed to optimize and validate methods for the analysis of thiamin and folic acid in fortified rice, pure and mixed to the milled rice (raw and cooked). The analysis was performed by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Different mobile phases were tested. Different ratios of organic modifier, pH ranges, triethylamine concentrations, and flow rates were used. For the validation, tests of recovery, repeatability, linearity, limit of detection (… Show more

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Cited by 3 publications
(1 citation statement)
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“…The suspension was centrifuged at 10,000 rpm for 15 min at 4°C and clarified by filtration through 0.22 μm syringe filters. The folic acid content of this filtrate was analysed by HPLC as described previously (C. M. M. Silveira, Della Lucia, Pirozi, Montini, & Pinheiro‐Sant'Ana, 2016). Briefly, reverse phase chromatography of folic acid was performed on a C‐18 column (Thermo‐Hypersil ODS, 5 μ, 250 × 4.6 mm) connected to a HPLC equipped with an online UV monitor and controlled by the Chromequest software (Thermo scientific, USA).…”
Section: Methodsmentioning
confidence: 99%
“…The suspension was centrifuged at 10,000 rpm for 15 min at 4°C and clarified by filtration through 0.22 μm syringe filters. The folic acid content of this filtrate was analysed by HPLC as described previously (C. M. M. Silveira, Della Lucia, Pirozi, Montini, & Pinheiro‐Sant'Ana, 2016). Briefly, reverse phase chromatography of folic acid was performed on a C‐18 column (Thermo‐Hypersil ODS, 5 μ, 250 × 4.6 mm) connected to a HPLC equipped with an online UV monitor and controlled by the Chromequest software (Thermo scientific, USA).…”
Section: Methodsmentioning
confidence: 99%