Methods for Assessment of the Glomerular Filtration Rate in Laboratory Animals

Gu, Xiangyang; Yang, Baoxue

doi:10.1159/000525049

Cited by 10 publications

(6 citation statements)

References 84 publications

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“…Despite our results showing improvement of renal fibrosis and ferroptosis markers in tissue level and kidney functional parameters such as UACR by mPTD-BMP7, other parameters did not match the tissue status. Although a persistent decrease in eGFR (estimated glomerular filtration rate) or increase in UACR are the commonly accepted parameters in diagnosing diabetic nephropathy ( 73 ), GFR is not usually appropriate in laboratory animal model ( 74 ). However, other parameters such as BUN, serum creatinine, and urinary albumin secretion were not significantly improved.…”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein-7 attenuates pancreatic damage under diabetic conditions and prevents progression to diabetic nephropathy via inhibition of ferroptosis

et al. 2023

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BackgroundApproximately 30% of diabetic patients develop diabetic nephropathy, a representative microvascular complication. Although the etiological mechanism has not yet been fully elucidated, renal tubular damage by hyperglycemia-induced expression of transforming growth factor-β (TGF-β) is known to be involved. Recently, a new type of cell death by iron metabolism called ferroptosis was reported to be involved in kidney damage in animal models of diabetic nephropathy, which could be induced by TGF-β. Bone morphogenetic protein-7 (BMP7) is a well-known antagonist of TGF-β inhibiting TGF-β-induced fibrosis in many organs. Further, BMP7 has been reported to play a role in the regeneration of pancreatic beta cells in diabetic animal models.MethodsWe used protein transduction domain (PTD)-fused BMP7 in micelles (mPTD-BMP7) for long-lasting in vivo effects and effective in vitro transduction and secretion.ResultsmPTD-BMP7 successfully accelerated the regeneration of diabetic pancreas and impeded progression to diabetic nephropathy. With the administration of mPTD-BMP7, clinical parameters and representative markers of pancreatic damage were alleviated in a mouse model of streptozotocin-induced diabetes. It not only inhibited the downstream genes of TGF-β but also attenuated ferroptosis in the kidney of the diabetic mouse and TGF-β-stimulated rat kidney tubular cells.ConclusionBMP7 impedes the progression of diabetic nephropathy by inhibiting the canonical TGF-β pathway, attenuating ferroptosis, and helping regenerate diabetic pancreas.

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Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein-7 attenuates pancreatic damage under diabetic conditions and prevents progression to diabetic nephropathy via inhibition of ferroptosis

et al. 2023

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“…After recovering from sevoflurane anesthesia, the NIC-Kidney device had no impact on movement or behavior of the animals. This indicates a low level of stress in the rats in contrasts to the stress induced by classical clearance studies, which require repeated restrainment and blood sampling [ 40 ]. Another advantage of the tGFR method is that it does not rely on accurately timed urine sampling making it a more efficient and faster technique compared with creatinine clearance studies, which often require 24-hour urine collection.…”

Section: Discussionmentioning

confidence: 99%

Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy

et al. 2023

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Objective Glomerular filtration rate (GFR) is a key indicator of renal function. In both clinical practice and pre-clinical research, serum levels of endogenous filtration markers, such as creatinine, are often used to estimate GFR. However, these markers often do not reflect minor changes in renal function. In this study, we therefore set out to evaluate the applicability of transcutaneous GFR (tGFR) measurements to monitor the changes in renal function, as compared to plasma creatinine (pCreatinine), in two models of obstructive nephropathy, namely unilateral ureteral obstruction (UUO) or bilateral ureteral obstruction followed by release (BUO-R) in male Wistar rats. Results UUO animals showed a significant reduction in tGFR compared to baseline; whereas pCreatinine levels were not significantly changed. In BUO animals, tGFR drops 24 h post BUO and remains lower upon release of the obstruction until day 11. Concomitantly, pCreatinine levels were also increased 24 h after obstruction and 24 h post release, however after 4 days, pCreatinine returned to baseline levels. In conclusion, this study revealed that the tGFR method is superior at detecting minor changes in renal function as compared to pCreatinine measurements.

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“…Говорячи про функціональну активність систем організму тварин, важко виділити структури, які мають більшу чи меншу значиму роль у забезпеченні гомеостазу (Gąsiorowski et al, 2019;Billman, 2020). Не можна не відзначити особливе значення нирок, що беруть участь у сечоутворенні та виконують досить різноманітні функції завдяки особливій будові паренхіми (Gu & Yang, 2022).…”

Section: результати та їх обговоренняunclassified

Morphological characteristics of the kidneys of mature dogs (Canis Lupus Familiaris L., 1758)

Khomenko,

Sokulskyi,

Zaika

2024

SMLNUVMB

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Each biological species of animals is unique in its structure, which is due to the uniqueness of ecological habitats and the peculiarities of adaptation of homeostasis mechanisms to the influence of environmental factors. Subtle differences in the structural and functional components of organs found in different species of animals create a clear architecture for understanding the depth of biological processes. Research of species, breed and age macro- and microanatomical structures and their morphometric indicators of organs and systems should be considered as urgent tasks of modern morphology. The new data on the morphological indicators of organs discovered at the macro- and micro-levels make a certain contribution to the age-related, breed-related, comparative, experimental and clinical morphology of mammals. Urinary organs in the body of mammals play one of the leading roles in maintaining the physiological and biochemical status of the organism and homeostasis as a whole, which largely depends directly on the morphological and functional state of these organs. Increasingly, there is a need for comprehensive scientific studies of various departments of the urinary organs. The scientific article uses material that is a fragment of the planned research work of the department of normal and pathological morphology, hygiene and expertise “Development, morphology and histochemistry of animal organs in normal and pathological conditions”, state registration number 0120U100796. The article contains the results of the morphometric characteristics of the kidneys of sexually mature dogs – Canis lupus familiaris L., 1758. During the implementation of this work, complex research methods were applied: anatomical, histological, morphometric and statistical. For histological examination, a fragment of kidneys was selected, they were fixed in 10 % neutral formalin solution, Carnois and Buena fluid. In the future, dehydration was carried out in alcohols of increasing concentration and the procedure for sealing the organ was carried out according to generally accepted methods. Using a sled microtome, histological sections with a thickness of 7–10 μm were obtained. Processing of the results of histological sections was examined under a “Micros” light microscope with a digital camera at a magnification of × 100–400. The morphological study of the structural organs of the urinary system was studied on histological sections by the method of light microscopy in accordance with the requirements of the international principles of the “European Convention for the Protection of Vertebrate Animals Used in Experiments and Other Scientific Purposes” (Strasbourg, 1986), by the decision of the First National Congress on Bioethics (M. Kyiv, 2001), Law of Ukraine No. 692 “On the Protection of Animals from Cruel Treatment” (3447-IV) dated February 21, 2006. According to the histological structure, the kidneys of dogs are represented by renal corpuscles and renal tubules, which are made of epithelial cells. Organometric studies indicate that the absolute mass of kidneys in sexually mature dogs is 119.8 ± 6.48 g, relative – 0.74 ± 0.04 %. Morphometric studies of the kidney parenchyma in dogs have established that the average area of renal corpuscles is 4.89 ± 0.24 thousand μm², the area of the vascular glomerulus is 3.79 ± 0.18 thousand μm², the area of the Shumlyansky-Bowman capsule lumen is 1.12 ± 0.27 thousand μm². The average volume of renal bodies of the cortical substance is 1265.69 ± 239.34 thousand μm³. The number of renal corpuscles per conventional unit of area (5 mm²) is 20.8 ± 0.6 units. The aim of the work was to investigate the peculiarities of the morphogistological structure of the kidneys of sexually mature dogs. The research results will expand information about the morphological structure, and will serve for the development of issues of physiology, pathology and treatment of diseases of the urinary organs.

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Methods for Assessment of the Glomerular Filtration Rate in Laboratory Animals

Cited by 10 publications

References 84 publications

Bone morphogenetic protein-7 attenuates pancreatic damage under diabetic conditions and prevents progression to diabetic nephropathy via inhibition of ferroptosis

Bone morphogenetic protein-7 attenuates pancreatic damage under diabetic conditions and prevents progression to diabetic nephropathy via inhibition of ferroptosis

Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy

Morphological characteristics of the kidneys of mature dogs (Canis Lupus Familiaris L., 1758)

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