Methods for calculating the stereotaxic coordinates of rat brain structures by pixel coordinates of the image obtained by confocal and two-photon laser scanning microscopy

Towards detection of cancer biomarkers in human exhaled air by transfer-learning-powered analysis of odor-evoked calcium activity in rat olfactory bulb

“…Surgical procedures were the same as in Ref. [ 37 ]. Since background adaptation and sensitivity to target odors over odorous backgrounds are qualitatively similar in awake and anesthetized animals [ 38 ] and experimental conditions in anesthetized rodents are experimentally stable both mechanically and physiologically comparing to awake animals [ 39 ], we used anesthetized rats in our study.…”

Where do you measure the Bregma for rodent stereotaxic surgery?

“…The key to surgery success is knowing the technique limitations and performing careful coordinate standardization. Also, it is important to point out that for long-term skull implants, increased caution is required regarding the stereotaxic coordinates due to deformation of brain surface after opening the cranial window ( Arefev et al, 2021 ). In this case, Arefev et al (2021) suggested a mathematical adjustment of stereotaxic coordinates based on the Bregma and the intersection of blood vessels on brain surface.…”