Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk

Abstract

Němečková I., Solichová K., Roubal P., Uhrová B., Šviráková E. (2011) Totally 75 raw milk samples were analysed with the methods employing the media compared -MYPA, PEMBA, Brilliance TM Bacillus cereus agar, and HiCrome Bacillus agar. The reference method with MYPA seems to be the most suitable for dairy plants laboratories because there is only low risk of mistaken identity. However, the samples containing miscellaneous micro-flora should be heat-inactivated before plating. Both positive and negative strains (totally 132) were isolated. Twelve strains, which could cause problems in the evaluation of the plates, were selected and identified by phenotyping and by PCR methods for Bacillus sp., B. cereus, and B. licheniformis. The PCR methods differed in their selectivity within particular bacilli group, within genera Bacillus, and within raw milk microflora.

Production and estimation of alkaline protease by immobilized Bacillus licheniformis isolated from poultry farm soil of 24 Parganas and its reusability

“…Gram staining [Figure 1] and other biochemical assays [Figure 2] according to Bergey's manual[16] was used in identifying the soil isolate, namely B. licheniformis [Table 1] which was at par with Němečková et al . [15] and Vigneshwaran et al .,[6] respectively.…”

“…Brilliance ™ Bacillus cereus agar and HiCrome Bacillus agar. [15] Subsequently microscopic and biochemical analysis as par Bergey's manual[16] was done for further identification of the isolated microbe.…”

Brewing microbiology - bacteria of the genera Bacillus, Brevibacillus and Paenibacillus and cultivation methods for their detection - part 2

“…For the diagnostics of B. licheniformis are used the same culture media as for B. cereus, e.g. MYP, PEMBA or TSB (Němečková et al, 2011;Salkinoja-Salonen et al, 1999). On MYP and PEMBA agar, B. licheniformis forms medium-sized yellow to yellow-green colonies with irregular edges with a diameter up to 5 mm (Němečková et al, 2011).…”

“…MYP, PEMBA or TSB (Němečková et al, 2011;Salkinoja-Salonen et al, 1999). On MYP and PEMBA agar, B. licheniformis forms medium-sized yellow to yellow-green colonies with irregular edges with a diameter up to 5 mm (Němečková et al, 2011). To determine the presence of the spores, thermal inactivation is performed at 80 °C for 10 minutes.…”

“…Pro diagnostiku B. licheniformis jsou používána stejná živná média jako v případě B. cereus, tedy např. MYP, PEMBA či TSB (Němečková et al, 2011;Salkinoja-Salonen et al, 1999). Na MYP a PEMBA agaru tvoří B. licheniformis středně velké žluté až žluto-zelené kolonie s nepravidelnými okraji o průměru do 5 mm (Němečková et al, 2011).…”