Methods for determining neurotransmitter metabolism markers for clinical diagnostics

Veselova, I. A.; Sergeeva, Elena A.; Makedonskaya, Maria I.; Eremina, Olga E.; Kalmykov, Stepan N.; Шеховцова, Т. Н.

doi:10.1134/s1061934816120108

Cited by 21 publications

(3 citation statements)

References 103 publications

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“…In addition to classical neurotransmitters, several proteins, including neuropeptides, are an interesting target for monitoring neurological diseases. SERS immunoassays serve as powerful techniques for protein detection, particularly in POC diagnosis and monitoring, showcasing high sensitivity for the quantitative detection of protein biomarkers at low concentrations. − These sensing mechanisms excel in identifying proteins associated with various neurological events, from assessing traumatic brain injuries (TBI) to monitoring neurodegenerative diseases like AD, often showcasing clinically acceptable LODs. Utilizing binding interactions between antibodies and target proteins ensures high specificity, which facilitates rapid and noninvasive detection of proteins.…”

Section: Protein Sensing With Sers Immunoassaysmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Nanosensing and Imaging in Neuroscience

Boudries,

Williams,

Paquereau--Gaboreau

et al. 2024

ACS Nano

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Monitoring neurochemicals and imaging the molecular content of brain tissues in vitro, ex vivo, and in vivo is essential for enhancing our understanding of neurochemistry and the causes of brain disorders. This review explores the potential applications of surface-enhanced Raman scattering (SERS) nanosensors in neurosciences, where their adoption could lead to significant progress in the field. These applications encompass detecting neurotransmitters or brain disorders biomarkers in biofluids with SERS nanosensors, and imaging normal and pathological brain tissues with SERS labeling. Specific studies highlighting in vitro, ex vivo, and in vivo analysis of brain disorders using fit-for-purpose SERS nanosensors will be detailed, with an emphasis on the ability of SERS to detect clinically pertinent levels of neurochemicals. Recent advancements in designing SERS-active nanomaterials, improving experimentation in biofluids, and increasing the usage of machine learning for interpreting SERS spectra will also be discussed. Furthermore, we will address the tagging of tissues presenting pathologies with nanoparticles for SERS imaging, a burgeoning domain of neuroscience that has been demonstrated to be effective in guiding tumor removal during brain surgery. The review also explores future research applications for SERS nanosensors in neuroscience, including monitoring neurochemistry in vivo with greater penetration using surface-enhanced spatially offset Raman scattering (SESORS), near-infrared lasers, and 2-photon techniques. The article concludes by discussing the potential of SERS for investigating the effectiveness of therapies for brain disorders and for integrating conventional neurochemistry techniques with SERS sensing.

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Section: Protein Sensing With Sers Immunoassaysmentioning

confidence: 99%

Surface-Enhanced Raman Scattering Nanosensing and Imaging in Neuroscience

Boudries,

Williams,

Paquereau--Gaboreau

et al. 2024

ACS Nano

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“…The focus in selecting target analytes was on neurotransmitters and their metabolites, which reportedly change or have the potential to be altered via brain stimulation [25][26][27][28][50][51][52][53]. Herein, 23 neurochemicals were analyzed, including catecholamines, tryptophan metabolites, Glu, GABA, and ACh.…”

Section: Target Substances and Standard Solutionsmentioning

confidence: 99%

Diagnostic Strategies for Brain Doping in an Animal Model via Quantitative Analysis of Neurochemicals

Cho,

Jeon,

Lee

et al. 2023

Separations

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Brain doping is a novel form of doping that involves stimulating specific brain regions to enhance sports performance. However, to the best of our knowledge, there is currently no established provision or detection method for it. As brain stimulation ultimately induces alterations in neurochemical concentrations, this study aimed to develop a diagnostic strategy for brain doping. We successfully developed and validated a sensitive simultaneous analysis method for 23 neurochemicals present in urine. Simple derivatization was employed to overcome ionization efficiency, enabling the effective detection of all the target compounds within 5 min. Additionally, we developed an animal model system using rats to replicate brain-doping scenarios and establish a diagnostic strategy. Behavior tests confirmed improved sports performance in the brain stimulation group. By examining changes in the distribution patterns of the target substances in urine samples, we observed that neurochemicals could be used as potential biomarkers for brain-doping diagnosis. The developed method allows the effective simultaneous analysis of multiple neurochemicals in biological samples and is expected to have various applications, including doping control. Thus, changes in the distribution pattern of neurochemicals could serve as a basis for brain-doping diagnosis.

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“…In addition to a change in levels in patients with acute appendicitis (Maghsoudi et al, 2021). The progression of neurodegenerative diseases can also be monitored by measuring 5‐HIAA levels, which are directly linked to the amount of excreted 5‐HA (Veselova et al, 2016). An analysis of HVA and 5‐HIAA in the cerebrospinal fluid of primates has already been conducted using HPLC with electrochemical detection to assess the exposure of animals to high levels of oral manganese (Mn) (Golub et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Analytical method for urinary homovanillic acid and 5‐hydroxyindoleacetic acid levels using HPLC with electrochemical detection applied to evaluate children environmentally exposed to manganese

Cardoso

Rocha

Souza-Júnior

et al. 2023

Biomedical Chromatography

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Homovanillic acid (HVA) and 5‐hydroxyindoleacetic acid (5‐HIAA) are the urinary metabolites of dopamine (DA) and serotonin (5‐HA), respectively. We aimed to develop an extraction method for the determination of HVA and 5‐HIAA, using strong anionic exchange cartridges combined with HPLC with electrochemical detection, and apply it to measure the levels of HVA and 5‐HIAA in children living near a ferro‐manganese alloy plant in Simões Filho, Brazil. The validated method showed good selectivity, sensitivity, precision, and accuracy. The limits of detection (LOD) were 4 and 8 μmol/L for 5‐HIAA and HVA, respectively, in urine. Recoveries ranged from 85.8 to 94%. The coefficients of determination (R2) of the calibration curves were greater than 0.99. Spot urine samples of 30 exposed children and 20 nonexposed ones were processed accordingly. The metabolite levels in exposed and reference children were within the physiological ranges. The medians (range) for 5‐HIAA and HVA of the exposed ones were 36.4 μmol/L (18.4–58.0) and 32.9 μmol/L (<LOD—91.9), respectively. There was no significant difference between the values presented by children in the reference group: 25.7 μmol/L (19.9–81.4) and 35.2 μmol/L (<LOD—67.6) for 5‐HIAA and HVA, respectively. These results suggest that the quantification of the urinary metabolites possibly does not reflect the interference of manganese in the metabolism of DA and 5‐HA in the central nervous system.

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Methods for determining neurotransmitter metabolism markers for clinical diagnostics

Cited by 21 publications

References 103 publications

Surface-Enhanced Raman Scattering Nanosensing and Imaging in Neuroscience

Surface-Enhanced Raman Scattering Nanosensing and Imaging in Neuroscience

Diagnostic Strategies for Brain Doping in an Animal Model via Quantitative Analysis of Neurochemicals

Analytical method for urinary homovanillic acid and 5‐hydroxyindoleacetic acid levels using HPLC with electrochemical detection applied to evaluate children environmentally exposed to manganese

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