Methods for imaging individual cilia in living echinoid embryos

Morris, Robert L.; Pope, Hans William.; Sholi, Adam N.; Williams, Leah M.; Ettinger, Chelsea R.; Beacham, Gwendolyn M; Shintaku, Tatsushi; Abbott, Zachary; Doherty, Elyse M.

doi:10.1016/bs.mcb.2014.12.004

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…The total number of cilia was counted along with total number of nuclei for at least 80 cells. Cilia length was measured using Fiji 55 as described in 56 .…”

Section: Methodsmentioning

confidence: 99%

The primary cilium is necessary for the differentiation and the maintenance of human adipose progenitors into myofibroblasts

Arrighi

Lypovetska

Moratal³

et al. 2017

Sci Rep

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The primary cilium is an organelle, present at the cell surface, with various biological functions. We, and others, have shown that it plays a role in the differentiation of adipose progenitors (APs) into adipocytes. APs can also differentiate into myofibroblasts when treated with TGF-β1. Several components of the TGF-β1 pathway are located within the cilium suggesting a function for this organelle in AP myofibrogenesis. We studied differentiation of APs into myofibroblasts in two human models: APs of the adipose tissue (aAPs) and APs resident in the skeletal muscles (mAPs). We showed that, in vivo, myofibroblasts within muscles of patients with Duchenne Muscular Dystrophy were ciliated. In vitro, myofibroblasts derived from APs maintained a functional primary cilium. Using HPI4, a small molecule that inhibits ciliogenesis, and siRNA against Kif-3A, we provide evidence that the primary cilium is necessary both for the differentiation of APs into myofibroblasts and the maintenance of the phenotype. Disruption of the primary cilium inhibited TGF-β1-signalisation providing a molecular mechanism by which the cilium controls myofibroblast differentiation. These data suggest that myofibroblasts from various origins are controlled differently by their primary cilium.

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“…The total number of cilia was counted along with total number of nuclei for at least 80 cells. Cilia length was measured using Fiji 55 as described in 56 .…”

Section: Methodsmentioning

confidence: 99%