Methods for Studying Soil Organic Matter

Chenu, Claire; Rumpel, Cornélia; Lehmann, Johannes

doi:10.1016/b978-0-12-415955-6.00013-x

Cited by 23 publications

(8 citation statements)

References 120 publications

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“…Though, it is key to know that not all of the organic materials block cementitious reaction. Some have no effects such as chloronaphthalene and others retard hydration reaction but do not affect soil strength gain such as ethylene glycol, benzoic acid, cellulose [93,94].…”

Section: Effect Of Organic Materialsmentioning

confidence: 99%

Fundamentals of soil stabilization

2017

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Section: Effect Of Organic Materialsmentioning

confidence: 99%

Fundamentals of soil stabilization

2017

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“…Physical and chemical fractionation methods are used to differentiate between functional SOC fractions containing SOC pools with different turnover times. Physical fractionation methods can be roughly categorized into aggregate and particle fractionations (7)(8)(9). Aggregate and particle fractionations isolate fractions by size, density or a mixture of both.…”

Section: Introductionmentioning

confidence: 99%

“…However, particle fractionations are based on the assumption that the associations between soil particles and their spatial arrangement are important for SOM dynamics because their bioaccessibility is a major driver of SOM decomposition (13). Therefore, particle fractionation methods are mostly used to isolate organic matter (OM) fractions with different turnover times (9,14,15). Particle density fractionation is applied to separate SOM that is not firmly associated with soil minerals.…”

Section: Introductionmentioning

confidence: 99%

“…These light fractions (LFs) consist of plant residues that are either free in the soil matrix or occluded in particles (13,15). Particle size fractionation is based on the concept, that decay induces fragmentation and OM formed during decomposition associates with minerals, preventing further decay (9,13). Particle density fractionations are often combined with particle size fractionations in fractionation schemes to collect additional information on LFs (16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

“…Particle density fractionations are often combined with particle size fractionations in fractionation schemes to collect additional information on LFs (16)(17)(18). While density fractionations allow a more differentiated separation of heavy and light fractions, simple particle size fractionations can be conducted faster and cheaper (8,9,13,14,(19)(20)(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

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A Simple Approach to Isolate Slow and Fast Cycling Organic Carbon Fractions in Central European Soils—Importance of Dispersion Method

et al. 2021

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Numerous approaches have been developed to isolate fast and slow cycling soil organic carbon (SOC) pools using physical and chemical fractionation. Most of these methods are complex, expensive, and time consuming and unsuited for high-throughput application, such as for regional scale assessments. For simpler and faster fractionation via particle size the key issue is the dispersion of soil. It is unclear how the initial dispersion of soil affects the turnover rates of isolated fractions. We investigated five commonly used dispersion methods using different intensities: shaking in water, shaking in water with glass beads, ultrasonication at 100 and 450 J ml−1 and sodium hexametaphosphate (Na-HMP). We used soils from long-term field experiments that included a change from C3 to C4 vegetation and adjacent control sites using δ13C isotope ratio mass spectrometry. We evaluated the degree of C3/C4 moieties of the fractions, mass and carbon recovery and reproducibility as well as the time expenditures of the dispersions, sieving and drying techniques to develop an efficient and cheap fractionation method. Our results indicate that ultrasonication as well as H2O treatment with and without glass beads resulted in fractions with different turnover. Moreover, isolation performances depended on soil texture. While the isolation of the fractions using water with and without glass beads was equivalent to ultrasonication in soils with low clay contents, these methods had limited potential for soils with high clay contents. Furthermore, treatment with water alone had less reproducible results than other tested methods. The SOC recovery was comparable and satisfactory amongst non-chemical dispersion methods and reached over 95% for each of these methods. The use of Na-HMP was unsuccessful due to high time expenditures and strong SOC leaching. We propose particle size fractionation combined with ultrasonic dispersion as a fast and highly reliable method to quantify slow and fast cycling SOC pools for a wide range of soil types and textures from agricultural sites in central Europe.

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