Methods of CD34+ cell separation: Comparative analysis

Beaujean, F.

doi:10.1016/s0955-3886(97)00018-0

Cited by 17 publications

(8 citation statements)

References 27 publications

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“…We report our results with bone marrow CD34+ cell enrichment using the CliniMACS system in eight children with IMSD. The median recovery of positively selected CD34+ cells was 46.2% with a purity of 97.5%, and a residual T cell content of 0.04 Â 10 6 . A median of 5.5 Â 10 6 /kg of CD34+ cells was infused.…”

Section: Discussionmentioning

confidence: 97%

“…Different techniques have been proposed to select CD34+ stem cells. 6 It has been previously shown that the magnetic-activated cell separation technique is highly effective for clinical-scale preparation of CD34+ progenitor cells collected from leukapheresis products. [7][8][9] Large-scale preparations of purified CD34+ cells from bone marrow (BM) products have been used and reported only occasionally, as the expected cell loss has been considered too high to safely perform HSCT, particularly in adult patients.…”

Section: Discussionmentioning

confidence: 99%

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Allogeneic bone marrow stem cell transplantation following CD34+ immunomagnetic enrichment in patients with inherited metabolic storage diseases

Gaipa

Dassi

Perseghin

et al. 2003

Bone Marrow Transplant

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Summary:T-cell depletion is an essential step in reducing the risk of graft-versus-host disease (GVHD) in patients with inherited metabolic storage diseases (IMSD) undergoing hematopoietic stem cell transplantation. This goal can be achieved either by selective removal of T cells or by positive selection of CD34+ cells. Large-scale preparations of purified CD34+ cells from bone marrow products have not been extensively described. We report our results with bone marrow CD34+ cell enrichment using the CliniMACS system in eight children with IMSD. The median recovery of positively selected CD34+ cells was 46.2% with a purity of 97.5%, and a residual T cell content of 0.04 Â 10 6 . A median of 5.5 Â 10 6 /kg of CD34+ cells was infused. All patients engrafted at a median time of 12 days and none of the patients developed GVHD. This method is technically feasible and can be successfully used to transplant children with IMSD.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Allogeneic bone marrow stem cell transplantation following CD34+ immunomagnetic enrichment in patients with inherited metabolic storage diseases

Gaipa

Dassi

Perseghin

et al. 2003

Bone Marrow Transplant

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“…Throughout the last decade, monoclonal antibodies against the human sialomucin CD34 [Krause et al, 1996] have been used to isolate HSPCs. These cells were initially selected using immunoadsorptive methods and fluorescence-activated cell sorting, but soon the selection of CD34+ HSPCs by paramagnetic bead-coupled antibodies became the method of choice in clinical stem cell transplantation [Beaujean, 1997].…”

Section: Clinical Valuementioning

confidence: 99%

New Insights into the Cell Biology of Hematopoietic Progenitors by Studying Prominin-1 (CD133)

Bauer

Fonseca²,

Florek³

et al. 2007

Cells Tissues Organs

124

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Prominin-1 (alias CD133) has received considerable interest because of its expression by several stem and progenitor cells originating from various sources, including the neural and hematopoietic systems. As a cell surface marker, prominin-1 is now used for somatic stem cell isolation. Its expression in cancer stem cells has broadened its clinical value, as it might be useful to outline new prospects for more effective cancer therapies by targeting tumor-initiating cells. Cell biological studies of this molecule have demonstrated that it is specifically concentrated in various membrane structures that protrude from the planar areas of the plasmalemma. Prominin-1 binds to the plasma membrane cholesterol and is associated with a particular membrane microdomain in a cholesterol-dependent manner. Although its physiological function is not yet determined, it is becoming clear that this cell surface protein, as a unique marker of both plasma membrane protrusions and membrane microdomains, might reveal new aspects of the cell biology of rare stem and cancer stem cells. The aim of this review is to outline the recent discoveries regarding the dynamic reorganization of the plasma membrane of rare CD133+ hematopoietic progenitor cells during cell migration and division.

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“…It is based on the principle that antibody molecules adsorbed onto polystyrene surfaces specifically bind to antigens present on the surface membrane of the cells to be selected or depleted from the sample [57]. For these purposes, polystyrene dishes are coated with antibodies specific for one or multiple cell-surface antigens, allowing the targeted cells to bind to the surface of said dishes.…”

Section: Cell Purification Technologiesmentioning

confidence: 99%

Cell Purification: A New Challenge for Biobanks

Almeida¹,

García‐Montero²,

Órfão³

2014

Pathobiology

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Performing ‘-omics' analyses on heterogeneous biological tissue samples, such as blood or bone marrow, can lead to biased or even erroneous results, particularly when the targeted cells and/or molecules are present at relatively low percentages/amounts. In such cases, whole sample analysis will most probably dilute and mask the features of the cell and/or molecules of interest, and this will negatively impact the results and their interpretation. Therefore, frequently it is critically important to have well-characterized and high-quality purified cell populations for the reliable detection of subtle variations in their specific features, such as gene expression profile, protein expression pattern and metabolic status. Biobanks are technological platforms which aim to provide researchers access to a large number of high-quality biological samples and their associated data, particularly to support high-quality scientific and clinical research projects, and such projects will benefit enormously by having access to high-quality purified cell populations or their biological components (e.g. DNA, RNA, proteins). Therefore, a clear opportunity exists for preparative cell sorting techniques in biobanks. Although multiple different cell purification approaches exist or are under development (e.g. cell purification techniques based on cell adherence, density and/or cell size properties, methods based on antibody binding as well as new lab-on-a-chip purification techniques), the choice for a specific technology depends on multiple variables, including cell recovery, purity and yield, among others. In addition, most cell purification approaches are not well suited for high-throughput (HT) purification of multiple cell populations coexisting in a sample. Here we review the most (currently) used cell sorting methods that may be applied for sample preparation in biobanks. For the different approaches, technical considerations about their advantages and limitations are highlighted, and the requirements to be met by a HT cell sorting technology to be used in biobanks are also discussed.

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Methods of CD34+ cell separation: Comparative analysis

Cited by 17 publications

References 27 publications

Allogeneic bone marrow stem cell transplantation following CD34+ immunomagnetic enrichment in patients with inherited metabolic storage diseases

Allogeneic bone marrow stem cell transplantation following CD34+ immunomagnetic enrichment in patients with inherited metabolic storage diseases

New Insights into the Cell Biology of Hematopoietic Progenitors by Studying Prominin-1 (CD133)

Cell Purification: A New Challenge for Biobanks

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