Methods of identifying sclareol content in the raw material of Salvia sclarea L. and in products of its processing

Timasheva, Lidia; Pekhova, O.A.; Danilova, I.L.

doi:10.21515/1999-1703-67-249-254

Cited by 2 publications

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“…Основные хозяйственные и морфометрические показатели (урожайность соцветий, массовую долю и сбор эфирного масла, массовую долю и сбор склареола, высоту растения, длину центрального соцветия, количество осей на центральном соцветии и количество боковых побегов) определяли по общепринятым методикам [28]. Массовую долю эфирного масла (МДЭМ) определяли методом гидродистилляции по Гинзбергу в соответствии с требованиями ГОСТ 34213, а массовую долю склареола в сырье шалфея -экстракционно-хроматографическим методом [29]. Коэффициент засухоустойчивости растений рассчитывали по показателям водного обмена [30].…”

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Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process

Stavtzeva¹,

Yegorova²

2021

TVAN

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Clary sage (Salvia sclarea L.) is a widely demanded essential oil plant. The products of its processing are used in medicine, perfumery and cosmetics, food industry, paint and varnish production. The main breeding tasks are to develop cultivars that combine high yield and quality of essential oil, resistant to abiotic and biotic stresses. All clary sage cultivars registered in the ‘State register of breeding achievements allowed for use’ Russian Federation were obtained on the basis of traditional methods. The initial breeding material of S. sclarea was obtained at the FSBSI “Research Institute of Agriculture of Crimea” using methods of cell engineering (induction of somaclones from callus cultures, obtaining hybrids in embryoculture and cell selection for resistance to osmotic stress). The aim of this work was to study the main economic and morphometric parameters of clary sage samples created using various biotechnological methods at the main stages of the breeding process. As a material for research, we used samples obtained in vitro using three biotechnological methods – regenerants (R2-R3) from calli of ‘C-785’ and ‘Taigan’ cultivars, obtained in isolated embryo culture hybrids (Salvia sclarea L. x S. grandiflora Etling.) and forms selected in embryoculture on a selective medium with an osmotic (mannitol or NaCl). When studying 10 sage samples (seed progeny of regenerants) in the breeding nursery (2012–2014), three that exceeded the control cultivar ‘Taigan’ in terms of basic economic characteristics were identified. The collection of essential oil from them was 1.2-1.4 times higher than in the control. In the nursery of competitive variety trials (2016–2019), when studying three samples (No. R3-1-6, 226-08 and 1-01), No. R3-1-6 was selected for a number of indicators. This sample is seed progeny of regenerant obtained by the induction of morphogenesis from callus. A characteristic feature of this sample was an increase in the number of lateral shoots per inflorescence by 41.1 %, which contributed to an increase in the mass of inflorescences. By the yield of inflorescences (162.0 c/ha) and the collection of essential oil (51.5 kg/ha), No. R3-1-6 exceeded control by 24.9 % and 43.9 %, respectively. The patent on the new clary sage cultivar ‘Selinzh’ (No. R3-1-6), in the creation of which the method of cell engineering was used for the first time, was received.

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Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process

Stavtzeva¹,

Yegorova²

2021

TVAN

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Description of a Collection of Salvia Sclarea L. In Terms of Its Biochemical Properties

Nevkrytaya,

Krivchik,

Krivda

et al. 2023

JCPRM

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The study object is the accumulation of essential oil in the inflorescences of clarysage, the content of its main components and the sclareol yield. The study purpose is to analyze a collection of clarysage (112 specimens) in terms of the basic biochemical properties (the essential oil content in raw material, its chemical composition and the sclareol yield) and to identify the specimens showing good prospects for inclusion in the breeding process. The study was carried out in 2018–2020 at the experimental plot of the Research Institute of Agriculture of Crimea located in the piedmont region of Crimea (KrymskayaRoza village, Belogorsk district). The climate is moderate continental. The average year-round temperature is +10 °С. The average year-round air humidity is 70%, the hydrothermic factor is 0.91. The accounting and analyses were performed in accordance with the techniques developed. The study revealed higher degree of genetic determination of the essential oil buildup in plants as compared to the weather effect. As noted, increased precipitation and extremely high temperatures during the plant flowering and seed formation time affect negatively the sclareol buildup. The variability of the properties analyzed (Cv=7.8–23.4%) bears evidence to a feasibility of selecting promising parent material for breeding. Based on the outcomes of the collection analysis there have been isolated 12 specimens leaving behind the varieties cultivated in terms of the basic biochemical properties including five specimens with a high mass fraction of essential oil – 0.90–1.06% on the dry basis (as compared to 0.6–0.88% in the varieties cultivated) and seven specimens with a high sclareol content in raw material – 2.00–2.35% (as compared to 1.58–1.71% in the varieties cultivated).

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Methods of identifying sclareol content in the raw material of Salvia sclarea L. and in products of its processing

Cited by 2 publications

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Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process

Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process

Description of a Collection of Salvia Sclarea L. In Terms of Its Biochemical Properties

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