Methotrexate induced apoptotic and necrotic chromatin changes in rat myeloid leukemia cells

Trencsényi, György; Bako, Fruzsina; Nagy, Gábor; Kertai, P; Bánfalvi, Gáspár

doi:10.1007/s00011-015-0797-x

Cited by 11 publications

(11 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to a recent study on oral mucositis in pediatric ALL patients, lip mucosa was the most frequently affected site, with the gingiva being a relatively uncommon site (Ribeiro, Limeira, Dias de Castro, & Ferreti Bonan, 2017). ALL is the most common and the most serious type of childhood cancer, accounting for about 80% of cases of leukemia between 0 and 19 years of age, and the prognosis for ALL is worse in adults over 30 years (Trencsenyi, Bako, Nagy, Kertai, & Banfalvi, 2015). Therefore, only a few prospective studies have analyzed the intraoral findings in adult ALL patients, and further studies are needed to support our results.…”

Section: Discussionmentioning

confidence: 99%

Prospective evaluation of clinical symptoms of chemotherapy‐induced oral mucositis in adult patients with acute leukemia: A preliminary study

Lee

Hong

Kim

et al. 2019

Clinical & Exp Dental Res

View full text Add to dashboard Cite

Objective The objective of this study was to prospectively evaluate the clinical features of chemotherapy‐induced oral mucositis (CIOM) in adult patients with acute leukemia and the aggravating factors for such symptoms. Subjects Thirty‐seven prospective patients aged ≥19 years with acute leukemia undergoing chemotherapy were enrolled. Oral and clinical investigations were performed at baseline and on Day 14 after starting chemotherapy. The presence and severity of cancer‐induced oral mucositis were demonstrated using the World Health Organization (WHO) scoring system. Results On Day 14, we found that oral mucositis had developed in eight patients (21.6%). Hematopoietic stem cell transplantation (HSCT) in patients was a predictor of increased WHO scores (β = 1.937, p < .001). Regarding oral sites, ventral tongue (β = 1.670), soft palate (β = 1.242), and buccal mucosa (β = 0.593) were predictors for increased scores. In addition, the increase in WHO scores was positively correlated with the number of oral lesions (r = .521), the difficulty in eating (r = .250), and the overall oral health (r = .534; all p < .05). Conclusion The main factors affecting the severity of CIOM symptoms were the treatment with HSCT and the location of oral lesions. The incidence of CIOM and WHO scores were not significantly different between the subgroups of disease. Our findings will help clinicians investigate the oral findings after chemotherapy in adult patients with acute leukemia.

show abstract

Section: Discussionmentioning

confidence: 99%

Prospective evaluation of clinical symptoms of chemotherapy‐induced oral mucositis in adult patients with acute leukemia: A preliminary study

Lee

Hong

Kim

et al. 2019

Clinical & Exp Dental Res

View full text Add to dashboard Cite

show abstract

“…Leukemia is considered the most prevalent underlying disease in pediatric patients and is characterized by the uncontrollable production of immature leukocytes [ 1 ]. Among existing types of leukemia, acute lymphoblastic leukemia (ALL) primarily affects leukocytes and is the most serious and most common type of childhood cancer, accounting for approximately 80% of cases of leukemia from 0 to 19 years of age [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Oral Mucositis in Pediatric Patients in Treatment for Acute Lymphoblastic Leukemia

Ribeiro

Limeira

Bonan

et al. 2017

IJERPH

View full text Add to dashboard Cite

Oral mucositis in oncologic patients is the most undesirable event of the chemotherapeutic treatment. This study aimed to identify damage to the oral cavity resulting from chemotherapy in pediatric patients with acute lymphoblastic leukemia (ALL). This is a prospective study with a sample of 42 children and adolescents evaluated for 10 consecutive weeks after diagnosis. The modified Oral Assessment Guide (OAG) was used, and data were analyzed by Spearman’s rank correlation coefficient (α = 5%). Changes to the normal lips and saliva were positively related to an increase in the OAG score during all 10 weeks of evaluation. Alterations to the labial mucosa were correlated with an increase in the OAG score from the 2nd to 10th week, which was also found for changes in the tongue and in the swallowing function in Weeks 1, 6, 8, 9, and 10 and for gum changes from the 5th to 7th week. No significant vocal changes were correlated with the total OAG score at any point during the monitoring period. Changes in lips, cheek and/or palatal mucosa, labial mucosa, and gum areas and in swallowing function were positively correlated with an increase in the severity of oral mucositis in patients with ALL after beginning chemotherapeutic treatment.

show abstract

“…2). Trencsenyi et al also found differences in chromatin conformation [36]. The correct separation of chromosomes is crucial for gamete formation and embryo development, the errors during separation will result in serious anomalies, for example, aneuploidy [25].…”

Section: Discussionmentioning

confidence: 99%

Methotrexate impaired in-vivo matured mouse oocyte quality and the possible mechanisms

Tian

et al. 2020

BMC Mol and Cell Biol

View full text Add to dashboard Cite

Background: Methotrexate (MTX) is an antifolate agent which is widely used in clinic for treating malignancies, rheumatoid arthritis and ectopic pregnancy. As reported, MTX has side effects on gastrointestinal system, nervous system and reproductive system, while its potential damages on oocyte quality are still unclear. It is known that oocyte quality is essential for healthy conception and the forthcoming embryo development. Thus, this work studied the effects of MTX on the oocyte quality. Results: We established MTX model mice by single treatment with 5 mg/Kg MTX. Both morphological and molecular biology studies were performed to assess the in-vivo matured oocytes quality and to analyze the related mechanisms. The in-vivo matured oocytes from MTX-treated mice had poor in-vitro fertilization ability, and the resulting embryo formation rates and blastocyst quality were lower than the control group. We found that the invivo matured MTX-treated mouse oocytes displayed abnormal transcript expressions for genes of key enzymes in the folate cycles. MTX increased the rate of abnormal chromosome alignment and affected the regulation of chromosome separation via disrupting the spindle morphology and reducing the mRNA expressions of MAD2 and Sgo1. MTX reduced the DNA methylation levels in the in-vivo matured oocytes, and further studies showed that MTX altered the expressions of DNMT1 and DNMT 3b, and may also affect the levels of the methyl donor and its metabolite. Conclusions: MTX impaired the in-vivo matured mouse oocyte quality by disturbing folate metabolism and affecting chromosome stability and methylation modification.

show abstract

Methotrexate induced apoptotic and necrotic chromatin changes in rat myeloid leukemia cells

Cited by 11 publications

References 32 publications

Prospective evaluation of clinical symptoms of chemotherapy‐induced oral mucositis in adult patients with acute leukemia: A preliminary study

Prospective evaluation of clinical symptoms of chemotherapy‐induced oral mucositis in adult patients with acute leukemia: A preliminary study

Oral Mucositis in Pediatric Patients in Treatment for Acute Lymphoblastic Leukemia

Methotrexate impaired in-vivo matured mouse oocyte quality and the possible mechanisms

Contact Info

Product

Resources

About