Methotrexate loaded poly(l-lactic acid) microspheres for intra-articular delivery of methotrexate to the joint

Drugs and Polymers for Delivery Systems in OA Joints: Clinical Needs and Opportunities

“…Some studies, however, showed beneficial effects of IA administration of paclitaxel and methotrexate without apparent toxicity and side effects in an animal model [61,62]. In line with other classes of drugs there is potential for cytostatic drugs when administered via an intra-articular drug delivery system [61].…”

“…For IA drug delivery, mostly the polymers that have proven to be biocompatible were investigated. PLA has been shown to be biocompatible in rabbit knees [61,62], polyethylene glycol (PEG), often combined with other polymers (e.g., polycaprolactone (PCL)) is biocompatible and able to control release characteristics of the incorporated drug [72][73][74][75], however, by far, the most used synthetic polymer is PLGA. This synthetic polymer has a good biocompatibility and is able to incorporate many different types of drugs [29,31,[35][36][37]39,[42][43][44][45][46]50,60,61,[72][73][74][76][77][78][79][80][81][82][83][84].…”

Intra-articular lornoxicam loaded PLGA microspheres: enhanced therapeutic efficiency and decreased systemic toxicity in the treatment of osteoarthritis

“…Lnxc in the rat plasma was extracted according to the method previously reported with modification (Liang et al 2004). Briefly, 2 ml acetonitrile was added to the sample (0.5 ml) vortex mixed for 2 min and centrifuged at 4000 rpm for 5 min, the supernatant was transferred Figure 3.…”

Enhanced targeting efficiency of PLGA microspheres loaded with Lornoxicam for intra-articular administration

“…Lnxc in the rat synovial fluid were extracted according to the method previously reported with modification (Liang et al 2004). Briefly, 2 ml acetonitrile was added to the sample (0.5 ml) vortex mixed for 2min and centrifuged at 4000rpm for 5min, the supernatant was transferred to glass tube and dried under a stream of nitrogen gas at 40°C.…”