Methyl bromide alters catecholamine and metabolite concentrations in rat brain

Honma, Tetsuo; Miyagawa, Masao; Sato, Mitsuo

doi:10.1016/0892-0362(87)90033-x

Cited by 15 publications

(3 citation statements)

References 20 publications

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“…All brain samples were stored at -80˚C until analysis and dissolved in 0.2 N HClO 4 containing 1 mM EDTA and 5 mM Na 2 S 2 O 5 before disruption using a PotterElvehjem homogenizer. The homogenate was centrifuged at 12,000 × g for 25 min at 4˚C, and the supernatant was used for analysis 17,18) .…”

Section: Extraction Of Brain Substancesmentioning

confidence: 99%

Alteration of Brain Levels of Neurotransmitters and Amino Acids in Male F344 Rats Induced by Three-week Repeated Inhalation Exposure to 1-Bromopropane

Suda

Honma²,

Miyagawa

et al. 2008

Ind Health

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The present study investigated the effects of 1-bromopropane (1BP) on brain neuroactive substances of rats to determine the extent of its toxicity to the central nervous system (CNS). We measured the changes in neurotransmitters (acetylcholine, catecholamine, serotonin and amino acids) and their metabolites or precursors in eight brain regions after inhalation exposure to 1BP at 50 to 1,000 ppm for 8 h per day for 7 d per week for 3 wk. Rats were sacrificed at 2 h (Case 1), or at 19 h (Case 2) after the end of exposure. In Case 1, the level of 5-hydroxyindoleacetic acid (5HIAA) was lowered in some brain regions by 1BP exposure. The decrease of 5HIAA in the frontal cortex was statistically significant at 50 ppm 1BP exposure. In Case 2, γ-amino butyric acid (GABA) and taurine were decreased in many brain regions of exposed rats, and a significant decrease of taurine in the midbrain occurred at 50 ppm 1BP exposure. In both cases of 2-h and 19-h intervals from the end of exposure to sacrifice, aspartate and glutamine levels were elevated in many brain regions, but the acetylcholine level did not change in any brain region. Three-week repeated exposure to 1BP produced significantly changes in amino acid contents of rat brains, particularly at 1,000 ppm.

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Section: Extraction Of Brain Substancesmentioning

confidence: 99%

Alteration of Brain Levels of Neurotransmitters and Amino Acids in Male F344 Rats Induced by Three-week Repeated Inhalation Exposure to 1-Bromopropane

Suda

Honma²,

Miyagawa

et al. 2008

Ind Health

Self Cite

View full text Add to dashboard Cite

show abstract

“…All brain samples were stored at -80°C and dissolved in 0.2 N HClO 4 containing 1 mM EDTA and 5 mM Na 2 S 2 O 5 before disruption using a Potter-Elvehjem homogenizer. The homogenate was centrifuged at 12,000 g for 25 min at 4°C, and the supernatant was analyzed by HPLC 20,21) . Supernatants were divided into two portions (Portion A & B).…”

Section: Extraction and Measurement Of Brain Substancesmentioning

confidence: 99%

Effects of Perinatal Exposure to Bisphenol A on Brain Neurotransmitters in Female Rat Offspring

Honma¹,

Miyagawa²,

Suda³

et al. 2006

Ind Health

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Pregnant Sprague-Dawley (CD IGS) rats were orally administered doses of bisphenol A (BPA) at 4, 40, and 400 mg/kg, from gestation days 6 to postnatal day 20. Neurotransmitters such as dopamine (DA) and serotonin (5HT) were extracted from the brains of dams and female offspring, and measured using liquid chromatography. BPA at 400 mg/kg was toxic and dosed rats died. At 3 wk after birth, brain levels of 3,4-dihydroxyphenylacetic acid (DOPAC, a DA metabolite), homovanillic acid (HVA, a DA metabolite), 5HT, 5-hydroxyindoleacetic acid (5HIAA, a 5HT metabolite) in female offspring were increased and the HVA/DA ratio was high in some brain areas of BPA-treated groups as compared with controls. At the age of 6 wk, levels of choline (Ch) in BPAtreated groups at 4 and 40 mg/kg were higher than control in all of eight brain areas. No changes were observed in acetylcholine (ACh) contents. In 9-wk-old offspring, changes in monoamines and metabolites were scattered and not great. At 3 wk after delivery, levels of 5HIAA in some brain areas of dams treated with BPA were higher than in control dams. Dose dependent increases in HVA and the HVA/DA ratio of the occipital cortex, and in the HVA/DA ratio of the frontal cortex were observed. The turnover of DA and 5HT was accelerated in 3-wk-old offspring and dams. BPA possesses very weak estrogenic activity. Changes in cerebral neurotransmitters observed in offspring and dams in this study may have been related to the estrogenic activity of BPA. However, further investigation is needed to examine the contribution of hormonal activity to such neurotransmitter changes.

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“…The alterations in neurotransmitters and their metabolites catecholamines and indoleamines in brain regions have been used as indexes of neurotoxicities of toxicants [11][12][13][14][15][16] . The ratios of the levels of neurotransmitters to those of their metabolites have been used as indexes of neurotransmitter turnover and neuronal activity 17) .…”

Section: The Alterations In Neurotransmitters and Their Metabolites Imentioning

confidence: 99%

The Alterations in Neurotransmitters and Their Metabolites in Discrete Brain Regions in the Rats after Inhalation of Disinfectant, Glutaraldehyde or ortho-phthalaldehyde for 4 Weeks

et al. 2011

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Glutaraldehyde (GA) and ortho-phtalaldehyde (OPA) have been widely used as major components of disinfectants in hospitals. We evaluated the alterations in GA or OPA in rats after subacute inhalation exposure by determining levels of neurotransmitters (norepinephrine Following the exposure, the brain of each rat was removed and dissected into cerebrum, cerebellum, medulla oblongata, midbrain, corpus striatum and hypothalamus. The neurotransmitters and their metabolites were extracted from each brain region, and determined by HPLC. Regarding GA, the daily water intake of the 50 or the 200 ppb exposed groups was significantly lower than that of the control. DA and 5-HIAA levels in the medulla oblongata among the GA exposed groups were significantly lower than those of the control. For OPA, the mean final body weight and daily food intake of the 100 or 200 ppb exposed groups were significantly lower than those of the control. The mean DA concentrations in the cerebrum in the groups exposed to OPA were significantly lower than those of the control. OPA may modulate DA metabolism in the cerebrum of female rats. The levels GA or OPA that induced alienations in neurotransmitters were comparable to those levels usually found in hospitals, further studies are warranted to evaluate the of safety of disinfectants containing GA or OPA.

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Methyl bromide alters catecholamine and metabolite concentrations in rat brain

Cited by 15 publications

References 20 publications

Alteration of Brain Levels of Neurotransmitters and Amino Acids in Male F344 Rats Induced by Three-week Repeated Inhalation Exposure to 1-Bromopropane

Alteration of Brain Levels of Neurotransmitters and Amino Acids in Male F344 Rats Induced by Three-week Repeated Inhalation Exposure to 1-Bromopropane

Effects of Perinatal Exposure to Bisphenol A on Brain Neurotransmitters in Female Rat Offspring

The Alterations in Neurotransmitters and Their Metabolites in Discrete Brain Regions in the Rats after Inhalation of Disinfectant, Glutaraldehyde or ortho-phthalaldehyde for 4 Weeks

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