Methyl Jasmonate Treatment Enhanced Protodioscin Biosynthesis in White Asparagus (Asparagus officinalis L.) Spears

Maeda, Tatsuaki; Jishi, Tatsuru; Komura, Akinori; Hasegawa, Hirokazu; Narita, Koji; Honda, Kentaro; Yamaguchi, Takayuki

doi:10.2503/jjshs1.81.337

Cited by 8 publications

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“…The samples of 18 different plant parts were pulverized for analysis. Rutin and protodioscin were extracted from 50 mg of freeze-dried powder with 1 mL of 70% ethanol according to the modification method of Maeda et al (2010Maeda et al ( , 2012 and Nikaido et al (2014). A highperformance liquid chromatography system (HPLC; Laboratory Solutions system; Shimadzu, Kyoto, Japan) equipped with an evaporative light scattering detector (ELSD-LTII detector; Shimadzu) and a photodiode array detector (SPD-M20A; Shimadzu) were used for quantification.…”

Section: Methodsmentioning

confidence: 99%

“…A highperformance liquid chromatography system (HPLC; Laboratory Solutions system; Shimadzu, Kyoto, Japan) equipped with an evaporative light scattering detector (ELSD-LTII detector; Shimadzu) and a photodiode array detector (SPD-M20A; Shimadzu) were used for quantification. HPLC conditions were as follows: 1.0 mL · min -1 flow rate, 40°C column temperature, a Waters Symmetry C18 column (4.6 · 250 mm; Nihon Waters K. K., Tokyo, Japan) with a security guard cartridge (3.0 · 4.6 mm), 0.1% trifluoroacetic acid: acetonitrile-water (by volume) mobile phase with a gradient condition, 354-nm wavelength of a photodiode array detector (Maeda et al, 2010(Maeda et al, , 2012Nikaido et al, 2014). A calibration curve was prepared using external standard rutin solution (Wako Pure Chemical Industries, Osaka, Japan) and protodioscin solution (Chroma-Dex Inc., Los Angeles, CA), and the rutin and protodioscin content were calculated from the peak area, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Asparagus spears contain abundant functional components, such as saponin and phenol carboxylic acids (Chin et al, 2002;Hartung et al, 1990;Wang et al, 2003). The representative functional components of asparagus include rutin (Chin et al, 2002;Maeda et al, 2005) and protodioscin (Brueckner et al, 2010;Chin et al, 2002;Lee et al, 2010;Maeda et al, 2012;Schwarzbach et al, 2006;Wang et al, 2003). Rutin has antiinflammatory and antihypertensive effects in animal experiments (Hellerstein et al, 1951;Selloum et al, 2003), and biological effects such as suppression of capillary weakness in humans (Griffith et al, 1944).…”

mentioning

confidence: 99%

“…Based on these findings, asparagus should be promoted as a rutin source, and cultivation techniques to increase the rutin content of asparagus may be developed in the future. On the other hand, principal factors influencing the quality of white asparagus spears are softness, sweetness, and bitterness (Brueckner et al, 2010;Hoberg et al, 2008;Maeda et al, 2012). According to Kawano et al (1975), the bitterness of white asparagus is derived from saponin.…”

mentioning

confidence: 99%

“…According to Kawano et al (1975), the bitterness of white asparagus is derived from saponin. Protodioscin, a typical saponin characterized by its pharmacological activity, is included in white asparagus spears (Brueckner et al, 2010;Chin et al, 2002;Lee et al, 2010;Maeda et al, 2012;Schwarzbach et al, 2006;Wang et al, 2003). Protodioscin was cytotoxic to some human cancer cell lines in vitro (Hu and Yao, 2002;Hibasami et al, 2003), and also has aphrodisiac properties (Gauthaman et al, 2002) and improved sexual function in animal experiments (Gauthaman et al, 2003).…”

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confidence: 99%

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Distribution of Rutin and Protodioscin in Different Tissue Parts of Asparagus (Asparagus officinalis L.)

Motoki¹,

Tang²,

Taguchi³

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Asparagus is a popular vegetable rich in healthy functional components. Asparagus spears are known to contain a large amount of rutin, which has been found to possess anti-inflammatory, antitumor, and antibacterial/viral properties, and protodioscin, which is an antitumor substance and present in the bottom parts (8 cm from the cut end). However, the process of its production leaves fern in the aboveground parts and roots in the underground parts as significant amounts of nonusable parts, and this issue should be solved. This study was conducted to examine the distributions of rutin and protodioscin, representative functional components in different parts of asparagus. The results suggested that large amounts of rutin were noted in the cladophylls and storage roots (brown and epidermis), and the protodioscin content was high in the buds, the soil-covered section of the spear, and the rhizome. A significant amount of rutin was detected in the aboveground parts, which is consistent with the results of previous studies, but it was also found in the storage roots. The largest amount of protodioscin was found in the buds, as well as in young fruits and seeds of the aboveground parts. Injury by continuously cropping asparagus may be associated with high rutin content in the storage roots of asparagus.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%