Methyl parathion-induced sperm shape abnormalities in mouse

Organophosphorous Pesticide Exposure Increases the Frequency of Sperm Sex Null Aneuploidy

“…Therefore, OP could induce sperm aneuploidies by interfering with chromosome migration during meiosis I and II, because they alkylate DNA and form adducts that may in turn be converted to interstrand crosslinks (16,18). In our study, overall hybridization efficiency was 99%.…”

“…On the other hand, it has been postulated that chemicals inducing crosslinks increase the probability of chromosomal nondisjunction (7,17). Therefore, OP could induce sperm aneuploidies by interfering with chromosome migration during meiosis because they alkylate DNA (16,18). Therefore, we aimed to assess the frequency of sperm aneuploidy for chromosomes X, Y, and 18 by means of three-color chromosome sperm-fluorescence in situ hybridization (FISH), and its relationship with urinary OP metabolites in men before and during pesticide spray season.…”

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

“…Epidemiological studies have linked exposure to OP (including Me-Pa) with decreased semen quality, increased sperm DNA damage and hormonal imbalance (Padungtod et al, 2000;Recio et al, 2001Recio et al, , 2005Sánchez-Peña et al, 2004). In addition, Me-Pa exposure caused testicular dysfunction (Narayana, 2006), reduced sperm quality (Mathew et al, 1992;Narayana et al, 2005), and increased sperm genetic damage (Narayana et al, 2005;Piña-Guzmán et al, 2006) in laboratory animals. The assessment of sperm quality is important but it is not the sole aspect for identifying and characterizing male reproductive toxicants.…”

“…Studies in men and animals have shown that Me-Pa alone, or in combination with other OP, alters male reproductive function, particularly semen quality, sperm chromatin condensation, and hormonal balance (Padungtod et al, 2000;Sánchez-Peña et al, 2004;Narayana et al, 2005;Piña-Guzmán et al, 2006). Several studies have reported the genotoxic potential of Me-Pa on sperm cells (Mathew et al, 1992;Narayana et al, 2005;Piña-Guzmán et al, 2006); however, the direct impact of in vivo exposure to Me-Pa on susceptible stages of spermatogenesis and the consequences on functional parameters and fertilizing ability of spermatozoa has not been determined yet. In the current study, we investigated the effects of male exposure to Me-Pa on the function and fertilizing capacity of male germ cells and their relationship with oxidative damage.…”

“…Concern for its adverse effects on human reproduction has increased because of its genotoxic effect in somatic (Rupa et al, 1990) and sperm cells (Mathew et al, 1992;Piña-Guzmán et al, 2006). Studies in men and animals have shown that Me-Pa alone, or in combination with other OP, alters male reproductive function, particularly semen quality, sperm chromatin condensation, and hormonal balance (Padungtod et al, 2000;Sánchez-Peña et al, 2004;Narayana et al, 2005;Piña-Guzmán et al, 2006).…”