Methyl Selenol as a Precursor in Selenite Reduction to Se/S Species by Methane-Oxidizing Bacteria

Eswayah, Abdurrahman S.; Hondow, Nicole; Scheinost, Andreas C.; Merroun, Mohamed L.; Romero-González, María; Smith, Thomas J.; Gardiner, Philip H. E.

doi:10.1128/aem.01379-19

Cited by 30 publications

(21 citation statements)

References 62 publications

(83 reference statements)

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“…Here, as in other studies, HAADF-STEM-EDX was used to study the distribution of metals in bacterial cells ( 33 , 43 , 44 ). The formation of cell-associated structures composed of precipitated metal ions has been observed previously, such as chromium-containing particles on the surface of a chromium(VI)-reducing Pseudomonas synxantha strain ( 45 ) and extracellular fibers and stalks of Fe(III) oxides formed by Gallionella ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

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Detoxification, Active Uptake, and Intracellular Accumulation of Chromium Species by a Methane-Oxidizing Bacterium

Enbaia

Eswayah

Hondow

et al. 2021

Appl Environ Microbiol

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Despite the wide-ranging proscription of hexavalent chromium, chromium (VI) remains among the major polluting heavy metals worldwide. Aerobic methane-oxidizing bacteria are widespread environmental microorganisms that can perform diverse reactions using methane as the feedstock. The methanotroph Methylococcus capsulatus Bath, like many other microorganisms, detoxifies chromium (VI) by reduction to chromium (III). Here, the interaction of chromium species with M. capsulatus Bath was examined in detail by using a range of techniques. Cell fractionation and HPLC-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) indicated that externally provided chromium (VI) underwent reduction, and was then taken up into the cytoplasmic and membranous fractions of the cells. This was confirmed by X-ray photoelectron spectroscopy (XPS) of intact cultures that indicated negligible chromium on the surface of, or outside, the cells. Distribution of chromium and other elements within intact and sectioned cells, as observed via transmission electron microscope (TEM) combined with energy-dispersive X-ray spectroscopy (EDX), and electron energy loss spectroscopy (EELS), was consistent with the cytoplasm/membrane location of the chromium (III), possibly as chromium phosphate. The cells could also take up chromium (III) directly from the medium in a metabolically dependent fashion, and accumulate it within the cells. These results indicate a novel pattern of interaction with chromium species distinct from that observed previously with other microorganisms. They also suggest that M. capsulatus, and similar methanotrophs may contribute directly to chromium (VI) reduction, and accumulation in mixed communities of microorganisms that are able to perform methane-driven remediation of chromium (VI). Importance M. capsulatus Bath is a well characterised aerobic methane-oxidising bacterium that has become a model system for biotechnological development of methanotrophs to perform useful reactions for environmental clean-up, and making valuable chemicals and biological products using methane gas. Interest in such technology has increased recently owing to increasing availability of low-cost methane from fossil, and biological sources. Here, it is demonstrated that the ability of this versatile methanotroph to reduce the toxic contaminating heavy metal chromium (VI) to less toxic chromium (III) form occurs at the same time as accumulation of the chromium (III) within the cells. This is expected to diminish the bioavailability of the chromium, and make it less likely to be re-oxidised to the toxic chromium (VI). Thus M. capsulatus has the capacity to perform methane-driven remediation of chromium-contaminated water, and other materials, and to accumulate the chromium in the low-toxicity chromium (III) form within the cells.

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Section: Discussionmentioning

confidence: 99%

“…Methanotrophs respond to lanthanide elements that are required for activity of a key metabolic methanol dehydrogenase ( 25 , 30 , 31 ). They also convert selenite (SeO 3 2- ) to selenium-containing nanoparticles and volatile methylated selenium species ( 32 , 33 ).…”

Section: Introductionmentioning

confidence: 99%

Detoxification, Active Uptake, and Intracellular Accumulation of Chromium Species by a Methane-Oxidizing Bacterium

Enbaia

Eswayah

Hondow

et al. 2021

Appl Environ Microbiol

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“…MPV1) [69], indicate that the same Se-S interaction may take place at the NP-OM interface, which could be partially responsible for PL properties of these SeNPs. It is worth noting that to better unravel the possible occurance of Se-S interactions at the NP surface, Raman spectroscopy for the detection of typical Se-S stretching vibrations is advisable [56,[84][85]; however, this aspect goes beyond the scope of the present study.…”

Section: Photoluminescence Properties and Lifetime Measurements Of Bimentioning

confidence: 94%

Tunable photoluminescence properties of selenium nanoparticles: biogenic versus chemogenic synthesis

et al. 2020

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AbstractVarious technological and biomedical applications rely on the ability of materials to emit light (photoluminescence [PL]), and, among them, metal nanoparticles (NPs) and semi-conductor Quantum Dots (QDs) represent ideal candidates as sensing probes and imaging tools, portraying better PL features than conventional organic dyes. However, the knowledge of PL behavior of semiconductor NPs – i.e., selenium; SeNPs – is still in its infancy, especially for those synthesized by microorganisms. Considering the essential role played by biogenic SeNPs as antimicrobial, anticancer, and antioxidant agents, or food supplements, their PL properties must be explored to take full advantage of them as eco-friendly and versatile tools. Here, PL features of SeNPs produced by the Se-tolerant Stenotrophomonas maltophilia SeITE02 strain, compared with chemogenic ones, are investigated, highlighting the PL dependency on the NP size. Indeed, PL emission shifted from indigo-blue (emission wavelength λem 400–450 nm) to green-yellow (λem 480–570 nm) and orange-red (λem 580–700 nm) for small (ca. 50 nm) and big (ca. 100 nm) SeNPs respectively, revealing the versatility of an environmental bacterial isolate to synthesize diverse PL probes. Besides, biogenic SeNPs show PL lifetime comparable to those of the most used fluorophores, supporting their potential application as markers for (bio)imaging.

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“…This technique may also be used to investigate the nature of organic matter (proteins, lipids, polysaccharides, etc.) attached to biogenic nanoparticles of interest (Eswayah et al, 2019;Fischer et al, 2020;Ruiz-Fresneda et al, 2020), which could affect their interactions and hence their environmental fate. For instance, the presence of absorption bands at 1659 and 1538 cm -1 corresponding to C-O, N- H and C-N vibrations attributed to amide I and amide II groups in purified selenium nanoparticles from Stenotrophomonas bentonitica suggested the presence of proteins surrounding them (Ruiz-Fresneda et al, 2020).…”

Section: Fourier Transform Infrared (Ftir) Spectroscopymentioning

confidence: 99%

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

López-Fernández

Jroundi

Ruiz-Fresneda

et al. 2020

Microbial Biotechnology

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Radionuclides (RNs) generated by nuclear and civil industries are released in natural ecosystems and may have a hazardous impact on human health and the environment. RN-polluted environments harbour different microbial species that become highly tolerant of these elements through mechanisms including biosorption, biotransformation, biomineralization and intracellular accumulation. Such microbial-RN interaction processes hold biotechnological potential for the design of bioremediation strategies to deal with several contamination problems. This paper, with its multidisciplinary approach, provides a state-of-theart review of most research endeavours aimed to elucidate how microbes deal with radionuclides and how they tolerate ionizing radiations. In addition, the most recent findings related to new biotechnological applications of microbes in the bioremediation of radionuclides and in the long-term disposal of nuclear wastes are described and discussed.

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Methyl Selenol as a Precursor in Selenite Reduction to Se/S Species by Methane-Oxidizing Bacteria

Cited by 30 publications

References 62 publications

Detoxification, Active Uptake, and Intracellular Accumulation of Chromium Species by a Methane-Oxidizing Bacterium

Detoxification, Active Uptake, and Intracellular Accumulation of Chromium Species by a Methane-Oxidizing Bacterium

Tunable photoluminescence properties of selenium nanoparticles: biogenic versus chemogenic synthesis

Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

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