Methylation Analysis of CpG Islands in Pineapple SERK1 Promoter

Luan, Aiping; Chen, Chengjie; Xie, Tongxin; He, Jun; He, Yehua

doi:10.3390/genes11040425

Cited by 13 publications

(11 citation statements)

References 33 publications

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“…At 60% FC, PP2C and PAL which have CpG islands in their promoter region showed lower levels of expression than HSP70 and RH25 which do not contain CpG island. A recent study on pineapple suggested that, DNA methylation at CpG islands in the promoter regions of a gene promoting somatic embryogenesis, SERK1 represses its expression (Luan et al ., 2020). Another study on transgenic flax crop showed that the gene chalcone synthase was more accessible to DNA methylation when located within CpG islands and the expression of the gene depended on the methylation status in these regions (Dzialo et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice

Sapna¹,

Ashwini²,

Ramesh³

et al. 2020

Plant Genet. Resour.

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DNA methylation is known to regulate gene expression when plants are exposed to abiotic stress such as drought. Therefore, insight into DNA methylation pattern would be useful for a better understanding of the expression profile of genes associated with drought adaptation. In the present study, we attempted to analyse the DNA methylation pattern at the whole-genome level and the expression of a few drought-responsive genes in rice under different regimes of soil water status, i.e. puddled, 100 and 60% field capacities (FC). The methylation-sensitive randomly amplified polymorphic DNA analysis was employed to identify DNA methylation pattern. We observed an increase in DNA methylation at 60% FC, and reduced methylation under 100% FC compared to puddled condition. The genes such as protein phosphatases (PP2C) and phenylalanine ammonia-lyase (PAL) having CpG islands in their promoter region had lower expression level under 100 and 60% FC compared to puddled conditions. Heat shock protein 70 (HSP70) and RNA helicase 25 (RH25), with no CpG islands in their promoter region, exhibited enhanced expression compared to puddled plants. In rice, increased DNA methylation seems to be an important mechanism associated with drought responses, which probably regulates the methylation-sensitive gene expression. The drought-induced changes in DNA methylation would contribute for epigenetic mechanism. The study provided evidence to argue that drought-induced increased methylation might be one of the major mechanisms associated with acclimation responses in field crops like rice.

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Section: Discussionmentioning

confidence: 99%

Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice

Sapna¹,

Ashwini²,

Ramesh³

et al. 2020

Plant Genet. Resour.

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“…The gene of an expressed protein (Ananas β-Actin) served as internal control. This was previously proposed as reference gene (Luan et al, 2020).…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

Effect of Micropropagation Conditions on Adventitious Buds Formation and the Circadian Expression of the ACO013229.1 Gene in Ananas Comosus

Ahmed

El-Fadl

Suliman

et al. 2021

Egyptian Journal of Desert Research

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mooth cayenne pineapple cultivar is considered the most suitable variety for the climatic conditions in Egypt, in addition to its distinctive flavor and ability for canning. To meet market demand, large quantities of plant materials are required, which cannot be obtained via traditional breeding methods. As a result, an in vitro technique was designed to increase the multiplication rate, rooting, and acclimatization of this unique pineapple variety. Thidiazuron (TDZ) at 2.0 mg/l proved to be superior for direct organogenesis rate. Half strength Murashige and Skoog (MS) medium containing 1.0 mg/l indole-3-butyric acid (IBA) in combination with 0.5 mg/l naphthalene acetic acid (NAA) improved the number and length of roots. Organogenesis has been accelerated from in vitro derived leaves and developed to healthy plantlets, which were acclimatized in the greenhouse. In order to investigate the effect of micropropagation on circadian rhythm, the circadian expression of Aco013229.1 was compared, which belongs to the MADS-box gene family, between the in vitro propagated plantlets and the in vivo-grown plants. The unaffected expression pattern of Aco013229.1 proposed that in vitro micropropagation did not affect the circadian cycling; hence, the CAM photosynthesis process was not interrupted. Moreover, the circadian expression of Aco013229.1 of the in vitro and in vivo-grown plants showed a similar pattern, strongly pointing at a stable circadian rhythm of the micropropagated plants and thus a well-maintained CAM photosynthesis. This gene family plays a significant role in a number of biological processes especially flowering.

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“…[2] It is a self-sterile plant, and the propagation methods widely used is from suckers. [3 The traditional propagation of suckers facilitate the production of pineapple but has enormous disadvantages, such as low rate of multiplication, long period of production, high variation in size, weight and age, and non-uniform vegetative cycle. [3 -4] Therefore, tissue culture have become an alternative methods for mass production of healthy pineapple planting material with homogeneity of morphotypes.…”

Section: -Introductionmentioning

confidence: 99%

Plant regeneration through somatic embryogenesis in two cultigroups of Pineapple (Ananas comosus L.)

Cacaï

Ahokpossi

Houédjissin

et al. 2022

Preprint

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Pineapple production is mostly constrained by unavailability of high-performance suckers. However, somatic embryogenesis (SE) have been revealed the most rapid and controllable method for Pineapple propagation than conventional sucker production methods. The aim of this study was to evaluate the responses of two cultivars of pineapple regenerated through somatic embryogenesis. Thus, calli were induced from crown leaf and plantlets leaf of Smooth Cayenne and Sugar Loaf cultivars. Murashige and Skoog base medium supplemented with vitamins B5 and different in hormonal combinations: Auxins / Cytokins. BAP and / or 2,4-D have been added to base medium for calli maturation and BAP and GA3 for plant regeneration. The results indicated a significant influence of type of explant and copper on callus induction in pineaple cultivars. Likewise, The medium MS with growth regulator combination NAA (0.5 mg/l) + BAP (1mg / l (BAP) has a highly significant influence with 8.8 mature somatic embryos. Also, the growth regulator combinations and the cultivars have significantly influenced somatic embryos regeneration with a high rate of 55.25% shoots by using the hormonal combination BAP (3 mg/l) + GA3 (2 mg/l) for the smooth Cayenne. The protocol developed is a key study for successful mass propagation of the pineapple and genetics transformation of pineapple.

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Methylation Analysis of CpG Islands in Pineapple SERK1 Promoter

Cited by 13 publications

References 33 publications

Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice

Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice

Effect of Micropropagation Conditions on Adventitious Buds Formation and the Circadian Expression of the ACO013229.1 Gene in Ananas Comosus

Plant regeneration through somatic embryogenesis in two cultigroups of Pineapple (Ananas comosus L.)

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