Methylene blue 1% as a sensitive and safe alternative for sentinel lymph node biopsy in early stage breast cancer: Results of a large pilot study

Khallaf, Emad; Abdoon, Mohamed; Lotfy, Abdelrahman; Abdalaziz, Youmna; Kamal, Rasha; Attia, Hazem Ali

doi:10.1111/tbj.13401

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…As a result, there is a clearly different MB uptake between normal and highly dysplastic/malignant cells. In spite of this limitation, MB staining is being widely used for diagnostic purposes in several types of cancer where it exhibits high selectivity and sensitivity [42][43][44]. The efficient binding of MB to the double helical DNA deduced from the absorption and fluorescence spectra [45][46][47], probably via intercalation, supports the idea that binding to DNA determines the signaling properties of MB [48][49][50].…”

Section: Resultsmentioning

confidence: 99%

Biomedical application of VIMP: screening of malignant cells in the prostate

Doménech‐Carbó

Doménech-Casasús²,

Pontones

et al. 2020

J Solid State Electrochem

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An electrochemical method for discriminating healthy and malignant tissues in prostate biopsies using the voltammetry of immobilized particles methodology is described. The method involves the sampling with graphite bars 0.5 mm in diameter on paraffin-impregnated cross sections of prostate tissues used in ordinary cytological evaluation after local staining with methylene blue (MB). The subsequent record of the voltammetric response of sample-modified graphite electrodes displays clearly different MB-centered features for healthy and malignant regions due to the different association of the dye to the respective cells.

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Section: Resultsmentioning

confidence: 99%

Biomedical application of VIMP: screening of malignant cells in the prostate

Doménech‐Carbó

Doménech-Casasús²,

Pontones

et al. 2020

J Solid State Electrochem

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“…Methylene blue (MB) has been used not only as a staining reagent but also as a NIR fluorescent material, emitting fluorescence at 700 nm [ 16 ]. Furthermore, this fluorescent dye, which is affordable and accessible and has low toxicity, helps to detect exposed SLN synchronously because of the blue color and fluorescence [ 11 , 17 , 18 , 19 ]. NIR fluorescence imaging using MB has been used to identify ureteral location [ 16 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Oral Sentinel Lymph Node Mapping by Near-Infrared Fluorescent Methylene Blue in Rats

Mao

Kang

et al. 2022

Diagnostics

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This study aimed to demonstrate the feasibility of near-infrared (NIR) fluorescence imaging using methylene blue (MB) for detecting oral sentinel lymph nodes (SLNs) in rats and compared MB’s tracer effects with those of indocyanine green (ICG) in SLN mapping. Different concentrations of MB were injected into the rats’ left lingual submucosa to determine the optimal concentration by using a continuous (1 h) MI-1 fluorescence imaging system. To compare the tracer effects of the optimal MB concentration with ICG in oral SLN mapping, MI-1 imaging was continuously monitored for 12 h. The mean signal-to-background ratio (SBR) of the SLNs and SLN fluorescence area fraction were analyzed. SLNs and lymphatic vessels were clearly visible in all rats. The optimal injection dose of MB infected into lingual submucosa for NIR fluorescence imaging was 0.2 mL of 6.68 mM MB. During continuous monitoring for 12 h, the mean SBR of the SLNs was significantly higher in the ICG groups than in the MB groups (p < 0.001). However, the area fraction of SLN fluorescence in the ICG groups increased continuously, owing to strong fluorescent contamination. This study examined the feasibility of detection of draining lymph nodes in the oral cavity of rats using MB NIR fluorescence imaging. MB causes less fluorescent contamination than does ICG, which shows promise for clinical research and application.

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