Methylene blue based protein solder for vascular anastomoses: An in vitro burst pressure study

Birch, Jeremy; Mandley, David John; Williams, Siân L.; Worrall, David R.; Trotter, Patrick J.; Wilkinson, Frances; Bell, P. R. F.

doi:10.1002/(sici)1096-9101(2000)26:3<323::aid-lsm11>3.0.co;2-r

Cited by 19 publications

(17 citation statements)

References 48 publications

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“…Such a process would also produce weak and unstable welds ( Figure 6A). [50] Naturally, the intensity of heat generation is also defined by lasing parameters (i.e., irradiance, fluence, pulse duration, and irradiation mode).…”

Section: Laser-solder Interactionsmentioning

confidence: 99%

“…Of importance is the manner in which a thermal gradient evolves between the nucleation centers (there where light absorption occurs, i.e., the chromophore molecules) and the non-absorbing regions as well as the consequent pattern of heat diffusion. More homogenous heating of the solder/tissue at lower chromophore concentrations produces a narrower temperature gradient, which has been associated with stronger repairs than welds made at higher dye concentrations, where heating is more heterogeneous and characterized by steeper thermal gradients ( Figure 6A, 8A) [50,56,63]. Alternative modulations of the thermodynamics during sLAVW include the separate application of chromophore and solder [57] and the employment of a solid solder film containing a chromophore gradient [58].…”

Section: Figurementioning

confidence: 99%

“…In addition to enhancing energy absorption, the color change of chromophores provides a visual cue to terminate irradiation or advancing lasing to an adjacent spot [50,63,88,89]. ICG turns tan, brown, and black to indicate smooth drying, gritty drying, and carbonization, respectively [63,88,89].…”

Section: Figurementioning

confidence: 99%

“…ICG turns tan, brown, and black to indicate smooth drying, gritty drying, and carbonization, respectively [63,88,89]. MB, on the other hand, turns white upon heating (i.e., the leucoform of MB), which additionally switches off MBmediated heat production [50]. Hence, based on the leucoform transition, MB has an advantage over ICG.…”

Section: Figurementioning

confidence: 99%

“…The technique is based on the sutureless coaptation of vessel segments by photothermal or photochemical [48] processes. Photothermal laser vessel bonding currently comprises the most frequently employed technique and can be divided into two distinct modalities, namely native laser-assisted vascular welding (LAVW) and modalities where proteinaceous solders are used to adjoin vessel segments, namely solder-enhanced LAVW (sLAVW) [49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64][65][66][67].…”

Section: Introductionmentioning

confidence: 99%

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Laser-assisted vessel welding: state of the art and future outlook

Pabittei

Boon

Heger

et al. 2015

JCTRes

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Laser-assisted vascular welding (LAVW) is an experimental technique being developed as an alternative to suture anastomosis. In comparison to mechanical anastomosis, LAVW is less traumatic, non-immunogenic, provides immediate water tight sealant, and possibly a faster and easier procedure for minimally invasive surgery. This review focuses on technical advances to improve welding strength and to reduce thermal damage in LAVW. In terms of welding strength, LAVW has evolved from the photothermally-induced microvascular anastomosis, requiring stay sutures to support welding strength, to sutureless anastomoses of medium-sized vessels, withstanding physiological and supraphysiological pressure. Further improvements in anastomotic strength could be achieved by the use of chromophore-containing albumin solder and the employment of (biocompatible) polymeric scaffolds. The anastomotic strength and the stability of welds achieved with such a modality, referred to as scaffold-and solder-enhanced LAVW (ssLAVW), are dependent on the intermolecular bonding of solder molecules (cohesive bonding) and the bonding between solder and tissue collagen (adhesive bonding). Presently, the challenges of ssLAVW include (1) obtaining an optimal balance between cohesive and adhesive bonding and (2) minimizing thermal damage. The modulation of thermodynamics during welding, the application of semi-solid solder, and the use of a scaffold that supports both cohesive and adhesive strength are essential to improve welding strength and to limit thermal damage.

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Section: Laser-solder Interactionsmentioning

confidence: 99%