Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea

Weon, Hang-Yeon; Kim, Byung-Yong; Joa, Jae–Ho; Son, Jung-A; Song, Myung-Hee; Kwon, Soon-Wo; Go, Seung-Joo; Yoon, Sang-Hong

doi:10.1099/ijs.0.65047-0

Cited by 75 publications

(35 citation statements)

References 9 publications

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“…Both strains had C 18:1 v7c as the major fatty acid. These were consistent with the major fatty acids of M. T (Kang et al, 2007;Madhaiyan et al, 2007a;Kato et al, 2008;Weon et al, 2008;Madhaiyan et al, 2009;Madhaiyan et al, 2012;Knief et al, 2012); however, strains BL36…”

supporting

confidence: 74%

Methylobacterium pseudosasicola sp. nov. and Methylobacterium phyllostachyos sp. nov., isolated from bamboo leaf surfaces

Madhaiyan

Prabakaran

2014

International Journal of Systematic and Evolutionary Microbiology

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Two strains of Gram-negative, methylotrophic bacteria, isolated because of their abilities to promote plant growth, were subjected to a polyphasic taxonomic study. The isolates were strictly aerobic, motile, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. The chemotaxonomic characteristics of the isolates included the presence of C18 : 1ω7c as the major cellular fatty acid. The DNA G+C contents of strains BL36T and BL47T were 69.4 and 69.8 mol%, respectively. 16S rRNA gene sequence analysis of strains BL36T and BL47T placed them under the genus Methylobacterium, with the pairwise sequence similarity between them and the type strains of closely related species ranging from 97.2 to 99.0 %. On the basis of their phenotypic and phylogenetic distinctiveness and the results of DNA–DNA hybridization analysis, the isolates represent two novel species within the genus Methylobacterium , for which the names Methylobacterium pseudosasicola sp. nov. (type strain BL36T = NBRC 105203T = ICMP 17621T) and Methylobacterium phyllostachyos sp. nov. (type strain BL47T = NBRC 105206T = ICMP 17619T) are proposed.

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supporting

confidence: 74%

Methylobacterium pseudosasicola sp. nov. and Methylobacterium phyllostachyos sp. nov., isolated from bamboo leaf surfaces

Madhaiyan

Prabakaran

2014

International Journal of Systematic and Evolutionary Microbiology

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“…They have also been found in association with humans (2), and some members of this genus have increasingly been reported to be a cause of opportunistic infections in immunocompromised patients (22). The genus Methylobacterium currently comprises 28 described species (28,60). Several new species have been described during the last few years, including species from environments that have been analyzed for several decades, such as the plant phyllosphere (23,27,28,38,58).…”

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confidence: 99%

Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

Knief

Francès

Cantet

et al. 2008

Appl Environ Microbiol

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Bacteria of the genus Methylobacterium are widespread in the environment, but their ecological role in ecosystems, such as the plant phyllosphere, is not very well understood. To gain better insight into the distribution of different Methylobacterium species in diverse ecosystems, a rapid and specific cultivationindependent method for detection of these organisms and analysis of their community structure is needed. Therefore, 16S rRNA gene-targeted primers specific for this genus were designed and evaluated. These primers were used in PCR in combination with a reverse primer that binds to the tRNA Ala gene, which is located upstream of the 23S rRNA gene in the 16S-23S intergenic spacer (IGS). PCR products that were of different lengths were obtained due to the length heterogeneity of the IGS of different Methylobacterium species. This length variation allowed generation of fingerprints of Methylobacterium communities in environmental samples by automated ribosomal intergenic spacer analysis. The Methylobacterium communities on leaves of different plant species in a natural field were compared using this method. The new method allows rapid comparisons of Methylobacterium communities and is thus a useful tool to study Methylobacterium communities in different ecosystems.Bacteria of the genus Methylobacterium are facultative methylotrophs capable of growth on one-carbon compounds, such as methanol, methylamine, formaldehyde, and formate (16). The members of this genus of Alphaproteobacteria are ubiquitous in nature. They have been detected in soil, dust, freshwater, lake sediments, and the air and on plants (16). They have also been found in association with humans (2), and some members of this genus have increasingly been reported to be a cause of opportunistic infections in immunocompromised patients (22). The genus Methylobacterium currently comprises 28 described species (28, 60). Several new species have been described during the last few years, including species from environments that have been analyzed for several decades, such as the plant phyllosphere (23,27,28,38,58). This suggests that the diversity of Methylobacterium is still not fully known. The high diversity of species in the genus Methylobacterium and their broad distribution in nature raises questions of whether different species are adapted to certain environments and whether they play similar ecological roles in different environments. To address these questions, a better understanding of the distribution of Methylobacterium strains between and within different ecosystems and analysis of Methylobacterium community compositions and dynamics in natural habitats are necessary.One of the major habitats of Methylobacterium is represented by plants. Members of this genus have been detected by cultivation-dependent methods (8,12,39,45,59) and, to a lesser extent, by cultivation-independent methods (3,24,25,47,48) as epiphytic and endophytic colonizers of the plant phyllosphere, as intracellular colonizers of the buds of Scots pine (47), and as root-nod...

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“…Gelatin, starch, glycerol tributyrate, casein and aesculin are not hydrolysed. Hydrogen sulfide is not Weon et al (2008) and Kang et al (2007). Values are percentages of total fatty acids; 2, not detected.…”

mentioning

confidence: 99%

Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice

Madhaiyan

Prabakaran

Kwon

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27 T , isolated from leaf tissues of rice (Oryza sativa L. 'Dong-Jin'), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae, Methylobacterium fujisawaense and Methylobacterium mesophilicum; strain CBMB27 T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA-DNA hybridization experiments revealed low levels (,38 %) of DNA-DNA relatedness between strain CBMB27 T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) in strain CBMB27 T differed from those of close relatives. The major fatty acid of the isolate was C 18 : 1 v7c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA-DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium, it was concluded that strain CBMB27 T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27 T 5LMG 24361 T 5KACC 11716 T 5DSM 19779 T ).

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Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea

Cited by 75 publications

References 9 publications

Methylobacterium pseudosasicola sp. nov. and Methylobacterium phyllostachyos sp. nov., isolated from bamboo leaf surfaces

Methylobacterium pseudosasicola sp. nov. and Methylobacterium phyllostachyos sp. nov., isolated from bamboo leaf surfaces

Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice

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