Método Da Folha Destacada Para Avaliar Resistência Da Soja Ao Oídio

Kamikoga, Aurea Tomoko Matsumoto

doi:10.5380/rsa.v3i1.1050

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“…The main advantages of the detached leaf technique are space and host material saving to evaluate genotype collections, economy of inocula of the pathogens to be studied, ease and accuracy of observations, lowest risk of contamination, uniformity of the experimental unit, ease of control and manipulation of environmental conditions, and possibility of keeping the detached leaves, in some cases, for a longer period than the normal life of plant leaves, as well as keeping the most prominent features and biochemical functions of plant leaves, including perspiration, respiration, photosynthesis and protein synthesis (13).…”

Section: (5)mentioning

confidence: 99%

Reaction of wheat cultivars and differential lines to Puccinia triticina races in detached leaves

Turra

Reis

Barcellos³

2014

Summa phytopathol.

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353Leaf rust caused by Puccinia triticina (Pt) Eriks. is one of the major leaf diseases affecting wheat in Brazil. Damage caused to wheat by leaf rust can be assessed by using the normalized function Y = 1,000 -6.4 I (Y = yield kg/ha and I = leaf incidence) (16).The main strategies for leaf rust control have been the development and the cultivation of genetically resistant cultivars, as well as the application of fungicides to aboveground plant parts (16). Genetic resistance of wheat to Pt is not durable due to the high fungal variability, resulting in occurrence of at least one new virulent race at every wheat growing season (3).Resistant cultivars are developed by inoculating Pt in new lines of wheat. For wheat leaf rust, resistance is race-specific; thus, dominant races are individually inoculated into the material to be selected (3). This requires a lot of material, facilities to grow the plants, and facilities to maintain the pure races. Each Pt race should be maintained, multiplied and kept pure, preventing mixtures with other races. RESUMOPalavras-chave adicionais: Ferrugem da folha, raças fisiológicas, Triticum aestivum.O método de preservação de folhas de trigo destacadas , em placa de Petri, foi usado para inoculação e desenvolvimento do fungo Puccinia triticina, agente causal da ferrugem da folha do trigo. Foram comparadas as reações de 26 cultivares de trigo em plântulas cultivadas em vasos (in vivo) e em folhas destacadas (in vitro) inoculadas com quatro raças fisiológicas do patógeno. Inoculou-se também Turra, C.; Reis, E.M.; Barcellos, A.L. Reação de cultivares de trigo e de linhagens da série diferencial à raças de Puccinia triticina, em folhas destacadas. Summa Phytopathologica, v.40, n.4, p.353-357, 2014. 24 linhas da série diferenciadora de raças do fungo nas mesmas condições. Após a inoculação, o material foi mantido em câmara climatizada por 15 dias. A avaliação da reação foi realizada no 15º dia após a inoculação. Os resultados obtidos para cada raça nos genótipos avaliados confirmaram a eficiência do método de folha destacada na avaliação da reação de cultivares de trigo. Reaction of wheat cultivars and differential lines to Puccinia triticina races in detached leaves 10.1590/0100-5405/1993The technique of detached leaves can be an important tool to rationalize the evaluation of the reaction of cultivars in the determination of Pt races.This technique has been used in studies of host-pathogen interaction with obligate parasites, such as fungi that cause rusts. Grass leaves or leaf pieces (wheat, oats, barley, rice and others) have been allowed to float on water or sucrose-based solutions, with or without benzimidazole, cytokine or kinetin (4, 7,10,11,12,17,18).Mignucci (14), Felsenstein (8) and Felsenstein (9) mention that adaptation of the detached leaf method was implemented to monitor the sensitivity of Pt to different fungicides in Europe.Considering Pt new races, race determination in every season and resistance break in some cultivars, the search for new resistant cultivars requi...

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Section: (5)mentioning

confidence: 99%

Reaction of wheat cultivars and differential lines to Puccinia triticina races in detached leaves

Turra

Reis

Barcellos³

2014

Summa phytopathol.

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“…Outros autores verificam a eficiência deste método de inoculação (Xavier, 1997;Kamikoga, 2001;Rios et al, 2001;Souza, 2008 …”

Section: Caracterização Monospóricaunclassified

Caracterização e controle de colletotrichum spp. em seringueira (Hevea brasiliensis)

Hayer¹,

Fernan²

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III Ofereço VI AGRADECIMENTOSAo nosso Deus por me acompanhar em todo este processo da minha vida e pela oportunidade de estar aqui no Brasil.A Universidade Estadual Paulista "Júlio de Mesquita Filho" Campus de Botucatu, pela oportunidade de realizar o curso de mestrado.A liga de Chopp, Bar Valentino, Café iguana, UP clube, escola ILC, por me dar trabalho quando precisei.A empresa Cauchopar e FEPAF por me conceder a bolsa de estudo.Aos meus pais, irmãos, e minha namorada, pelo apoio incondicional, dedicação, força e amor.Ao meu amigo, pai, professor e orientador Furtado por seu apoio, confiança e atenção. À Sakata Seed Sudamerica por ceder os isolados padrões de Colletotrichum gloeosporioides (1) e Colletotrichum acutatum (2).Ao Instituto Biológico de São Paulo por ceder os isolados 19 e 27. À empresa Hevea -Tec e a todos seus empregados, por me permitir a oportunidade de estar por duas semanas aprendendo e coletando a coleção de isolados presentes neste trabalho, e pelo apoio em todas as viagens na região de São José do Rio Preto, SP. Aos pesquisadores da empresa Corpoica Colômbia por me apoiar: Juan Jaramillo, Javier Orozco, Lorenzo Peláes Suárez, Diego Aristizabal, Margarita Vélez, Anibal Tapiero, VII Jaime Triana, a meu amigo e professor Alvaro Caicedo (esposa e filhos), Elizabeth Narvaez e Anderson Narvaez.A minha tia Velarmina Guerrero, Saida Hayer, Leonor Sierra, Ruth Sierra, Esteban Sierra por me apoiar.Ao professor Cesar Martins por me apresentar a meus amigos de república e por ser tão boa pessoa.Aos amigos de laboratório: Martha Passador e Cris de Pieri por ser tão gente fina comigo, e pela grande amizade.À Fabiano Betelloni (Deixa falar) e Karolina Dória, por ser tão legais comigo, por estar nas horas ruins e boas, por sempre se preocupar, e por sempre me socorrer quando precisei. The study was carried out by six phases: a) culture characterization, in which the color and the culture appearance were observed in vitro, conidial production and growth rate at six temperatures (10, 15, 20, 25, 30, 35 ˚C); b) morphological characterization, which consist of measuring the length and width, and observed the shape of the conidia; c) pathogenicity test on selected leaves and disks of leaves, with four isolates from rubber and two isolates from citrus; d) growth in fungicide benomyl at four concentrations of active ingredient; e) molecular identification for monosporic and multisporic cultures with specific primers to the species of Colletotrichum gloeosporioides and Colletotrichum acutatum and ITS1 and ITS4primers which amplified a small region (18S) and a large region (28S), and these also allowed the amplification of 5.8S rDNA and internal transcribed spacers (ITS1 and ITS2); f) Growth were obtained: a) the isolates were divided into two groups, a first group with orange+gray and orange color, and a second group with light gray color. The appearance color was not a predominant factor to determine the species. The conidial production showed to be varied from one isolate to another. The optimum temperature fo...

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Método Da Folha Destacada Para Avaliar Resistência Da Soja Ao Oídio

Cited by 2 publications

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Reaction of wheat cultivars and differential lines to Puccinia triticina races in detached leaves

Reaction of wheat cultivars and differential lines to Puccinia triticina races in detached leaves

Caracterização e controle de colletotrichum spp. em seringueira (Hevea brasiliensis)

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