Método rápido de marcaje de leucocitos con 99mTc HM-PAO

Succinylated gelatine: an alternative to hydroxyethyl starch for labelling leukocytes with 99mTc-HMPAO

“…The final labelled cells showed analogous erythrocyte/ leukocyte ratios for both agents, similar to the results described by other authors [15,16].…”

Guidelines for the labelling of leucocytes with 99mTc-HMPAO

“…This takes 30 to 45 min. As an alternative method to separate erythrocytes from LRP, centrifugation at low speed has been described [20], but this method has not been widely applied yet. The centrifugation method is faster than the sedimentation method, but gives rise to more erythrocyte contamination.Collect the LRP in a Falcon centrifuge tube.…”