Mg alloy surface immobilised with caerin peptides acquires enhanced antibacterial ability and putatively improved corrosion resistance

Preclinical Pharmacokinetics, Biodistribution, and Acute Toxicity Evaluation of Caerin 1.9 Peptide in Sprague Dawley Rats

“…The H&E staining was followed as reported elsewhere [ 16 ]. Following formalin fixation, the important tissues were dehydrated, stained with hematoxylin (about 15 minutes) and eosin (1–3 minutes), dehydrated again with gradient alcohol, clarified in xylene, and then examined and recorded under the microscope [ 25 ].…”

“…e adrenal gland, brain (brain, cerebellum, and brain stem), epididymis, heart, kidney, liver, ovary, spleen, testis, thymus, thyroid gland, and uterus were carefully collected, weighed, and documented before being kept in 10% neutral formalin buffer (10% NBF). e H&E staining was followed as reported elsewhere [16]. Following formalin fixation, the important tissues were dehydrated, stained with hematoxylin (about 15 minutes) and eosin (1-3 minutes), dehydrated again with gradient alcohol, clarified in xylene, and then examined and recorded under the microscope [25].…”

“…Caerin 1.9 exhibits effective antibacterial activity without inducing resistance after 30 passages, and Caerin peptides in a temperature-sensitive gel formulation inhibited bacteria growth in the skin of MRSA-infected mice [ 14 , 15 ]. Another study found that its immobility property improves metal corrosion resistance significantly [ 16 ]. Caerin 1.1 and 1.9 inhibit the growth of a variety of cancer cells in vitro , including MCF-7 and SKBR-3 breast cancer cells, B-CPAP and CAL-62 thyroid cancer cells, and HPV+ cell lines TC-1 and Hela cells [ 17 – 19 ].…”

In vitro degradation, photo-dynamic and thermal antibacterial activities of Cu-bearing chlorophyllin-induced Ca–P coating on magnesium alloy AZ31

“…Obviously, the frequent postoperative infection of bone implants becomes one of the most serious complications, leading to implant failure and bacteria-infected organs [ 11 ]. At present, the methods to improve the antibacterial performance of biodegradable Mg alloys mainly include the addition of antibiotics [ 12 , 13 ], polypeptides [ 14 ], and co-doping with metal ions (substances that can release metal ions) [ [15] , [16] , [17] , [18] ]. Nevertheless, the antimicrobial resistance after using of antibiotics may limit its applications in vivo [ 19 , 20 ].…”

Strontium-Containing Barium Titanate-Modified Titanium for Enhancement of Osteointegration

“…The rearrangement of Ti 4+ and O 2– in BTO results in the negative charges accumulating on the BTO surface and positive charges concentrating inside it. Therefore, BTO can be used to reconstruct the electric microenvironment at the injured site of the bone and electrically induce bone rebuilding. − However, the electric microenvironment can be achieved by prepolarized BTO, and even when BTO is polarized, how long the microenvironment remains electrified is unknown. Moreover, without considering its piezoelectric property, BTO is an intrinsic insufficient bioactive material because it is not degradable, and the metal ions in the structure (Ti 4+ and Ba 2+ ) are difficult to release.…”