MICA polymorphism in a northern Chinese Han population: The identification of a new MICA allele, MICA*059

Distribution of MICA haplotypes in a Chinese Han population

“…Similar MICA frequencies were observed among different Chinese ethnic groups; with the exception of MICA*019, which was higher in frequency (14.93%) in our cohort compared with other Chinese populations (3.85 [22] and 9.5% [23]). In the northern Chinese population, Tian and co-workers reported a lower frequency of MICA*12:01, MICA*019 and a higher frequency of MICA*004 [24].…”

MICA genetic polymorphism and HLA-A,C,B,MICA and DRB1 haplotypic variation in a southern Chinese Han population: Identification of two new MICA alleles, MICA*060 and MICA*062

“…Maximum likelihood haplotype frequencies were estimated using the expectationmaximization (EM) algorithm, as implemented in the program ARLEQUIN 3.11. The classic coefficients of LD, ⌬, and of normalized LD, ⌬rel, were computed for each individual haplotype as described previously [11,14]. Fisher exact test was performed to determine the significance of ⌬rel.…”

“…In a recent study, MICA allelic variation was investigated in a northern Chinese Han population [11]. In the present study, we investigated MICA allelic distribution in a Chinese Han population in Hunan province, in which a novel MICA allele, MICA*060, was identified.…”

“…A comprehensive strategy was used for MICA typing, as recently described by us [11]. Briefly, polymerase chain reaction-sequencebased typing (PCR-SBT) was used for the analysis of MICA exons 2, 3, and 4, fluorescent polymerase chain reaction-fragment analysis was used to determine the (GCT)n microsatellite at exon 5 of MICA gene (abbreviated as MICA-STR) [4,11], and polymerase chain reaction-sequence-specific priming (PCR-SSP) was applied to detect MICA gene deletion (MICA*Del) [4,11].…”

“…To verify this polymorphism, PCR product of MICA exon 4 derived from sample W124 was therefore cloned using a TA cloning kit (TaKaRa, Dalian, China) [11]. The inserts of 20 randomly selected recombinant plasmids were sequenced in both directions.…”