Mice Selected for Acute Inflammation Present Altered Immune Response during Pristane-Induced Arthritis Progression

Corrêa, Mara Adriana; Borrego, Andrea; Jensen, José Ricardo; Cabrera, Wafa Hanna Koury; Barros, Michele Andrade de; Katz, Iana Suly Santos; Canhamero, Tatiane; Spadafora-Ferreira, Mônica; Fernandes, Jussara Gonçalves; Starobinas, Nancy; Ribeiro, Orlando; Ibañez, Olga M.; Franco, Marcelo De

doi:10.1155/2018/1267038

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…In this context, IL-12 is also an important cytokine for its ability to stimulate the functions of NK cells, macrophages, and T lymphocytes [ 22 ]. High CD8 + cell, IL-12, and IL-10 levels, and lower IFN- γ production in the AIRmax mice were previously observed in different situations [ 22 , 36 , 38 , 40 ] and demonstrated a delicate balance between the nature of the stimulus and the host response.…”

Section: Discussionmentioning

confidence: 77%

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Castoldi

Romagnoli

Golim

et al. 2022

International Journal of Inflammation

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AIRmax and AIRmin mice strains were selected according to the intensity of their acute inflammatory responsiveness. Previous studies have shown that AIR mice differ in their resistance to chemically induced skin tumors and in the development of melanoma metastases, in addition to differences in neutrophil and NK cells activity. In the present work, we aimed to evaluate whether the difference of susceptibility to murine melanoma is associated with NK cytotoxic activity against Yac.1 cells and lymphocyte subsets. Mice were subcutaneously inoculated with B16F10 or S91 melanoma cells. After 7, 14, or 30 days, the animals were euthanized to analyze the number of lymphocyte subsets, cytotoxic activity, and number of cytokine-producing spleen cells. AIRmax mice presented a higher number of CD4+/CD25+ cells than that of AIRmin mice following inoculation of B16F10 cells, whereas inoculation of S91 cells reduced CD4+/CD25+ and increased TCD8+ cell subsets in the AIRmax mice. AIRmax mice had a higher number of interleukin (IL)-10- and IL-12-producing cells and a lower number of interferon-γ–producing cells than those of AIRmin mice at 30 days. The cytotoxic activity of nonadherent spleen cells was similar in both the AIR strains. These results suggest that melanoma cells can induce different responses in AIR mice, possibly owing to alterations in regulatory mechanisms, such as the action of CD4+/CD25+ regulatory T cells and IL-10, in AIRmax mice.

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Section: Discussionmentioning

confidence: 77%

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Castoldi

Romagnoli

Golim

et al. 2022

International Journal of Inflammation

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“…Besides their central role in inflammasomes for cytokine release, ASC specks can be released to the extracellular space, accumulate in inflamed tissues and can be internalized by surrounding inflammatory cells inducing inflammation chronicity or amplification, therefore worsening the disease or contributing to cure (26,27). The AIRmax and AIRmin mouse lines differ in natural resistance to infections, to the induction of autoimmune diseases and chemical carcinogenesis as well as in tissue repair capacity (18,19,(28)(29)(30)(31)(32)(33)(34). These phenotypes might be influenced by the differential Irm1 locus-dependent inflammasome activation between the two lines described here.…”

Section: Discussionmentioning

confidence: 99%

Pycard and BC017158 Candidate Genes of Irm1 Locus Modulate Inflammasome Activation for IL-1β Production

Borrego

Colombo²,

Souza³

et al. 2022

Front. Immunol.

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We identified Pycard and BC017158 genes as putative effectors of the Quantitative Trait locus (QTL) that we mapped at distal chromosome 7 named Irm1 for Inflammatory response modulator 1, controlling acute inflammatory response (AIR) and the production of IL-1β, dependent on the activation of the NLRP3 inflammasome. We obtained the mapping through genome-wide linkage analysis of Single Nucleotide Polymorphisms (SNPs) in a cross between High (AIRmax) and Low (AIRmin) responder mouse lines that we produced by several generations of bidirectional selection for Acute Inflammatory Response. A highly significant linkage signal (LOD score peak of 72) for ex vivo IL-1β production limited a 4 Mbp interval to chromosome 7. Sequencing of the locus region revealed 14 SNPs between “High” and “Low” responders that narrowed the locus to a 420 Kb interval. Variants were detected in non-coding regions of Itgam, Rgs10 and BC017158 genes and at the first exon of Pycard gene, resulting in an E19K substitution in the protein ASC (apoptosis associated speck-like protein containing a CARD) an adaptor molecule in the inflammasome complex. Silencing of BC017158 inhibited IL1-β production by stimulated macrophages and the E19K ASC mutation carried by AIRmin mice impaired the ex vivo IL-1β response and the formation of ASC specks in stimulated cells. IL-1β and ASC specks play major roles in inflammatory reactions and in inflammation-related diseases. Our results delineate a novel genetic factor and a molecular mechanism affecting the acute inflammatory response.

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“…As a result of their divergent inflammatory response, we have demonstrated that AIRmax and AIRmin mice differ in their susceptibility to bacterial infections (17), tumorigenesis (18,19), and autoimmune diseases (20,21). With regard to systemic and local responses induced by BjV, both lines are equally susceptible to venom-induced lethality, showing similar LD 50 .…”

Section: Introductionmentioning

confidence: 94%

Pain and Cellular Migration Induced by Bothrops jararaca Venom in Mice Selected for an Acute Inflammatory Response: Involvement of Mast Cells

et al. 2022

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Bothrops jararaca venom (BjV) can induce mast cell degranulation. In order to investigate the role of mast cells and the interference of the host genetic background in the inflammation induced by BjV, we have used mouse strains selected for maximal (AIRmax) or minimal (AIRmin) acute inflammatory response (AIR). Mice were pretreated with an inhibitor of mast cell degranulation, cromolyn (CROM), and injected in footpads or intraperitoneally (i.p.) with BjV. Pain was measured with von Frey hairs, cell migration in the peritoneum by flow cytometry, and reactive oxygen species (ROS) production by chemiluminescence assays. The nociceptive response to BjV was higher in AIRmax than AIRmin mice; however, this difference was abolished by pretreatment with CROM. BjV induced peritoneal neutrophil (CD11b+ GR-1+) infiltration and ROS secretion in AIRmax mice only, which were partially inhibited by CROM. Our findings evidence a role for mast cells in pain, neutrophil migration, and ROS production triggered by BjV in AIRmax mice that are more susceptible to the action of BjV.

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Mice Selected for Acute Inflammation Present Altered Immune Response during Pristane-Induced Arthritis Progression

Cited by 8 publications

References 30 publications

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Pycard and BC017158 Candidate Genes of Irm1 Locus Modulate Inflammasome Activation for IL-1β Production

Pain and Cellular Migration Induced by Bothrops jararaca Venom in Mice Selected for an Acute Inflammatory Response: Involvement of Mast Cells

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